A dorsal cannulation technique is described. It has been employed for repeated blood sampling in unanaesthetized rainbow trout (Salmo gairdneri) kept singly in special receptacles described in the paper. The level of the studied haematological parameters (Haematocrit, Hb, glucose, lactate, K+, Na+, Ca2+) differed between fish kept in receptacles for 1 week and free-swimming fish, most probably owing to differences in the motility of the fish. The receptacle seems to minimize visual and handling disturbances, and permits both the standardization of experimental conditions and quick and easy sampling via the dorsal aorta cannula. The general variation in the blood parameter values was very small compared with the previously reported variation in such values for rainbow trout.
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JOURNAL ARTICLE| 01 August 1975
A technique for repeated sampling of the blood of individual resting fish
Online Issn: 1477-9145
Print Issn: 0022-0949
© 1975 by Company of Biologists
J Exp Biol (1975) 63 (1): 207–217.
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A. Soivio, K. Nynolm, K. Westman; A technique for repeated sampling of the blood of individual resting fish. J Exp Biol 1 August 1975; 63 (1): 207–217. doi: https://doi.org/10.1242/jeb.63.1.207
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