In humans, the cation channel TRPM2 (HsTRPM2) has been intensively studied because it is involved in oxidative stress mediated apoptosis and also contributes to temperature regulation. The gating mechanism of TRPM2 is quite complex where a C-terminally localized enzyme domain plays a crucial role. The analysis of orthologues of TRPM2, in particular from the distantly related marine invertebrate Nematostella vectensis (NvTRPM2) revealed that during evolution the functional role of the endogenous enzyme domain of TRPM2 has undergone fundamental changes. In this study we investigate whether these evolutionary differences also apply to the physiological functions of TRPM2. For this purpose, we generated a TRPM2 loss-of-function mutation in Nematostella and compared the phenotypes of wild-type and mutant animals after exposure to either oxidative stress or high temperature. Our results show that under standard culture conditions mutant animals are indistinguishable from wild-type animals in terms of morphology, and development. However, exposure of both experimental groups to different stressors revealed that TRPM2 sensitizes to oxidative stress but attenuates high temperature injury in Nematostella. Therefore, NvTRPM2 plays opposite roles in the cellular response to these two different stressors. These findings reveal a similar physiological spectrum of activity of TRPM2 in humans and Nematostella and open up the possibility to establish Nematostella as a model organism for the physiological function of TRPM2.
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RESEARCH ARTICLE|
24 February 2022
TRPM2 sensitizes to oxidative stress but attenuates high temperature injury in the sea anemone Nematostella vectensis
Wiebke Ehrlich,
Wiebke Ehrlich
1
Institute of Physiology, Medical Faculty, University hospital Aachen
, Germany
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James M. Gahan,
James M. Gahan
2
Sars International Centre for Marine Molecular Biology, University of Bergen.
, Norway
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Fabian Rentzsch,
Fabian Rentzsch
2
Sars International Centre for Marine Molecular Biology, University of Bergen.
, Norway
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Frank J.P. Kühn
Frank J.P. Kühn
*
3
University Hospital Aachen: Universitatsklinikum Aachen-University hospital, Aachen Pauwelsstr. 30, 52074 Aachen
, Germany
*Author for correspondence: fkuehn@ukaachen.de
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Wiebke Ehrlich
1
Institute of Physiology, Medical Faculty, University hospital Aachen
, Germany
James M. Gahan
2
Sars International Centre for Marine Molecular Biology, University of Bergen.
, Norway
Fabian Rentzsch
2
Sars International Centre for Marine Molecular Biology, University of Bergen.
, Norway
Frank J.P. Kühn
*
3
University Hospital Aachen: Universitatsklinikum Aachen-University hospital, Aachen Pauwelsstr. 30, 52074 Aachen
, Germany
*Author for correspondence: fkuehn@ukaachen.de
Received:
25 Oct 2021
Accepted:
21 Feb 2022
Online ISSN: 1477-9145
Print ISSN: 0022-0949
Funding Group:
- Award Group:
- Funder(s): German Research Foundation
- Award Id(s): KU-2271/4-3
- Funder(s):
J Exp Biol jeb.243717.
Article history
Received:
25 Oct 2021
Accepted:
21 Feb 2022
Currently Viewing Accepted Manuscript - Newer Version Available
23 Mar 2022
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Citation
Wiebke Ehrlich, James M. Gahan, Fabian Rentzsch, Frank J.P. Kühn; TRPM2 sensitizes to oxidative stress but attenuates high temperature injury in the sea anemone Nematostella vectensis. J Exp Biol 2022; jeb.243717. doi: https://doi.org/10.1242/jeb.243717
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