Morphotypic differentiation is the external manifestation of dominance hierarchy in Macrobrachium rosenbergii. The intermediate morphotype orange claw (OC) male exhibits the highest growth rate and is subordinate in hierarchy to blue claw (BC) male while dominant on small male (SM). The present study was undertaken to examine the specific role of insulin-like androgenic gland (iag) hormone in morphotype differentiation of M. rosenbergii. To achieve this, RNAi mediated knockdown as well as augmentation of iag transcripts were effected in ∼60g OC males using plasmid-based constructs pcD-IAG-lh and pcD-IAGorf, respectively. The treatments were administered to animals maintained in isolation as well as in community. The knockdown plasmid construct that expresses iag specific long hairpin RNA caused 16-fold reduction of iag transcripts in SSN1 cell line in vitro. When injected into OC males living in a community 2.3-fold iag knockdown was recorded, while in isolated OC males it was 4.2-fold initially, but returned to normal subsequently. Compared to the respective controls, OC to BC transformations in the iag silenced animals were significantly lower in the community-reared group, while no difference was observed in the isolated ones. It is reported here for the first time that iag augmentation in OC males resulted in significantly higher OC to BC transformations, when animals were reared in community. This plasmid-based IAG knockdown approach could be developed into a low stress, feed or immersion treatment for controlling heterogeneous individual growth of M. rosenbergii males in aquaculture.
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RESEARCH ARTICLE|
01 January 2017
Silencing and augmentation of IAG hormone transcripts in adult M. rosenbergii males affects morphotype transformation
Himanshu Priyadarshi,
Himanshu Priyadarshi
ICAR-Central Institute of Fisheries Education, Mumbai-400061, India
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Rekha Das,
Rekha Das
ICAR-Central Institute of Fisheries Education, Mumbai-400061, India
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Annam Pavan-Kumar,
Annam Pavan-Kumar
ICAR-Central Institute of Fisheries Education, Mumbai-400061, India
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Pathakota Gireesh-Babu,
Pathakota Gireesh-Babu
ICAR-Central Institute of Fisheries Education, Mumbai-400061, India
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Hasan Javed,
Hasan Javed
ICAR-Central Institute of Fisheries Education, Mumbai-400061, India
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Sujit Kumar,
Sujit Kumar
ICAR-Central Institute of Fisheries Education, Mumbai-400061, India
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Makesh Marappan,
Makesh Marappan
ICAR-Central Institute of Fisheries Education, Mumbai-400061, India
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Somdutt,
Somdutt
ICAR-Central Institute of Fisheries Education, Mumbai-400061, India
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Gopal Krishna,
Gopal Krishna
ICAR-Central Institute of Fisheries Education, Mumbai-400061, India
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Aparna Chaudhari
Aparna Chaudhari
*
ICAR-Central Institute of Fisheries Education, Mumbai-400061, India
*Author for correspondence:
Principal Scientist and Head, Division of Fish Genetics and Biotechnology, Central Institute of Fisheries Education, Indian Council of Agricultural Research, Versova, Mumbai-400061, India
. Email: achaudhari67@gmail.com
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Himanshu Priyadarshi
ICAR-Central Institute of Fisheries Education, Mumbai-400061, India
Rekha Das
ICAR-Central Institute of Fisheries Education, Mumbai-400061, India
Annam Pavan-Kumar
ICAR-Central Institute of Fisheries Education, Mumbai-400061, India
Pathakota Gireesh-Babu
ICAR-Central Institute of Fisheries Education, Mumbai-400061, India
Hasan Javed
ICAR-Central Institute of Fisheries Education, Mumbai-400061, India
Sujit Kumar
ICAR-Central Institute of Fisheries Education, Mumbai-400061, India
Makesh Marappan
ICAR-Central Institute of Fisheries Education, Mumbai-400061, India
Somdutt
ICAR-Central Institute of Fisheries Education, Mumbai-400061, India
Gopal Krishna
ICAR-Central Institute of Fisheries Education, Mumbai-400061, India
Aparna Chaudhari
*
ICAR-Central Institute of Fisheries Education, Mumbai-400061, India
*Author for correspondence:
Principal Scientist and Head, Division of Fish Genetics and Biotechnology, Central Institute of Fisheries Education, Indian Council of Agricultural Research, Versova, Mumbai-400061, India
. Email: achaudhari67@gmail.com
Received:
24 May 2017
Accepted:
22 Aug 2017
Online ISSN: 1477-9145
Print ISSN: 0022-0949
J Exp Biol jeb.163410.
Article history
Received:
24 May 2017
Accepted:
22 Aug 2017
Currently Viewing Accepted Manuscript - Newer Version Available
15 Nov 2017
Citation
Himanshu Priyadarshi, Rekha Das, Annam Pavan-Kumar, Pathakota Gireesh-Babu, Hasan Javed, Sujit Kumar, Makesh Marappan, Somdutt, Gopal Krishna, Aparna Chaudhari; Silencing and augmentation of IAG hormone transcripts in adult M. rosenbergii males affects morphotype transformation. J Exp Biol 2017; jeb.163410. doi: https://doi.org/10.1242/jeb.163410
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