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Keywords: siRNA
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Journal Articles
J Cell Sci (2018) 131 (10): jcs209932.
Published: 15 May 2018
... for the myofibroblast phenotype, and point to STAT3 as an important target in pulmonary fibrosis and other fibrotic diseases. Fibrosis siRNA Fibroblast Stiffness TGF-β Myofibroblasts are key effector cells in wound healing, but also play a leading role in pathological fibrosis and contribute to tumor...
Includes: Supplementary data
Journal Articles
J Cell Sci (2013) 126 (11): 2381–2391.
Published: 1 June 2013
... characterised, including the extracellular-signal-regulated kinases (ERKs) 1 and 2. In a small interfering RNA (siRNA)-based high-throughput wound healing screen performed in A549 lung carcinoma cells, we identified ERK2 but not ERK1 as a strong mediator of HGF-induced motility. This finding was confirmed...
Includes: Supplementary data
Journal Articles
J Cell Sci (2011) 124 (16): 2826–2836.
Published: 15 August 2011
... K. , Pham J. W. , Kim K. , He Z. , Sontheimer E. J. , Carthew R. W. ( 2004 ). Distinct roles for Drosophila Dicer-1 and Dicer-2 in the siRNA/miRNA silencing pathways . Cell 117 , 69 - 81 . Li X. , Carthew R. W. ( 2005 ). A microRNA mediates EGF...
Includes: Supplementary data
Journal Articles
J Cell Sci (2010) 123 (11): 1825–1839.
Published: 1 June 2010
... regulation mediated by small-interfering RNAs (siRNAs) and microRNAs (miRNAs). More recent research, however, has identified new classes of RNAs and new regulatory mechanisms, expanding the known regulatory potential of small non-coding RNAs to encompass chromatin regulation. In this Commentary, we provide...
Journal Articles
J Cell Sci (2010) 123 (3): 369–383.
Published: 1 February 2010
... Staufen1 HIV-1 Staufen1 HIV-1-dependent RNP SHRNP siRNA Ribonucleoprotein RNA encapsidation Virus-host interaction AIDS Intracellular traffic The mechanisms governing the encapsidation of retroviral genomic RNAs have been the subject of intense research for many years. The principal...
Includes: Supplementary data
Journal Articles
J Cell Sci (2009) 122 (17): 3070–3082.
Published: 1 September 2009
... Smoothened, Patched-1 and Gli2. Knockdown of the primary cilium by Ift88 and Ift20 siRNA or treatment with cyclopamine, an inhibitor of Smoothened, blocks hedgehog signaling in P19.CL6 cells, as well as differentiation of the cells into beating cardiomyocytes. E11.5 embryos of the Ift88 tm1Rpw (Ift88-null...
Includes: Supplementary data
Journal Articles
J Cell Sci (2007) 120 (4): 586–595.
Published: 15 February 2007
... 1δ/ϵ Dishevelled Wnt-5a Dopaminergic neurons Non-canonical Wnt signalling siRNA The Wnt signalling pathway is a highly conserved biochemical pathway that is involved in a vast array of processes in both embryonic development and adult tissue homeostasis (for reviews, see Huelsken...
Journal Articles
J Cell Sci (2006) 119 (23): 4986–4993.
Published: 1 December 2006
... that colocalizes with PSD95. Over-expression of ARHGEF6, of PAK3 or constitutively active PAK3 did not alter spine morphology. By contrast, knockdown of ARHGEF6 using a siRNA approach resulted in abnormalities in spine morphology similar to those reported with knockdown of PAK3. This phenotype could be rescued...
Journal Articles
J Cell Sci (2006) 119 (15): 3219–3226.
Published: 1 August 2006
... with microvascular disease in humans, we hypothesised that small interfering RNA (siRNA) targeting this immediate-early gene may be useful agents that suppress endothelial growth and neovascularisation. Here we show that Jun siRNA inhibits Jun mRNA and protein expression in murine microvascular endothelial cells...
Journal Articles
J Cell Sci (2006) 119 (7): 1266–1282.
Published: 1 April 2006
... protein-10 ( CHOP-10 ), mitochondrial glycerol-3-phosphate dehydrogenase ( GPDmit ), and stearoyl-CoA desaturase 1 ( SCD1 ). We also demonstrate that overexpression of two dominant negative mutants of the cAMP-response element-binding protein CREB (K-CREB and M1-CREB) and siRNA transfection, which disrupt...
Includes: Supplementary data
Journal Articles
J Cell Sci (2005) 118 (20): 4709–4720.
Published: 15 October 2005
..., silencing of RAMP1 in the endogenously expressing HEK293 cells using siRNA resulted in altered CaSR traffic. Taken together, our results show that the association with RAMPs is necessary and sufficient to transfer the immature CaSR retained in the ER towards the Golgi where it becomes fully glycosylated...
Includes: Supplementary data
Journal Articles
J Cell Sci (2005) 118 (18): 4103–4111.
Published: 15 September 2005
... phosphorylation of PECAM-1 as well as the serine phosphorylation of Akt and eNOS were abolished by the pre-treatment of cells with the tyrosine kinase inhibitor PP1 the phosphorylation of AMPK was unaffected. Down-regulation of PECAM-1 using a siRNA approach attenuated the shear-stress-induced phosphorylation...
Journal Articles
J Cell Sci (2005) 118 (6): 1173–1183.
Published: 15 March 2005
... of RNA interference (RNAi) using small interfering RNAs (siRNAs) to define the roles of the best-characterized members of the RhoGTPase family, RhoA, Rac1 and Cdc42, in the control of MMP-1, MMP-2 and type-I-collagen expression in normal human skin fibroblasts (HSFs). A specific and long-lasting...
Journal Articles
J Cell Sci (2005) 118 (4): 689–696.
Published: 15 February 2005
...Jens Gruber; Tina Lampe; Mary Osborn; Klaus Weber FACE 1 is the endoprotease responsible for cleavage of prelamin A to lamin A. Transfection of HeLa cells with siRNA for human FACE 1 results in a strong phenotype. Protein and mRNA levels for FACE 1 are knocked down and cell division stops abruptly...
Journal Articles
J Cell Sci (2005) 118 (2): 343–356.
Published: 15 January 2005
... abolished the influence of elastin-derived peptides (EDPs) on cell migration and tubulogenesis, thus favoring the involvement of MT1-MMP in such processes. To assess its contribution to EDP-mediated angiogenesis further, we used a small interfering RNA (siRNA) approach for specifically silencing MT1-MMP...
Journal Articles
J Cell Sci (2005) 118 (2): 433–446.
Published: 15 January 2005
... siRNA Neurite elongation and branching Microtubule-associated proteins Transfection efficiency was determined by using fluorescein-labeled siRNA. For these experiments, siRNA corresponding to Ror1 or Ror2 was labeled using the Label IT® siRNA Tracker™ Fluorescein Kit (Mirus, Madison, WI...
Includes: Supplementary data
Journal Articles
J Cell Sci (2004) 117 (24): 5905–5912.
Published: 15 November 2004
... migration and accelerated cell spreading. Knocking down PHI-1 protein expression using siRNAs had pronounced and specific effects on cell migration. Different sequences were used for synthesis of two siRNA molecules that yielded essentially identical phenotypes. This supported the idea...
Includes: Multimedia, Supplementary data
Journal Articles
J Cell Sci (2004) 117 (7): 1047–1054.
Published: 1 March 2004
..., two genes code for isoforms of topo II, termed α and β. They are similar in primary structure and have almost identical catalytic properties in vitro. We transfected HeLa cells with small interfering RNAs (siRNAs) targeted against either topo IIα or IIβ, and succeeded in knocking down the expression...
Journal Articles
J Cell Sci (2003) 116 (13): 2775–2779.
Published: 1 July 2003
... aggregates, with self-perpetuating properties, by binding and converting PrP-sen molecules into a likeness of themselves. In the present report, we show that small interfering RNA (siRNA)duplexes trigger specific Prnp gene silencing in scrapie-infected neuroblastoma cells. A non-passaged, scrapie-infected...
Journal Articles
J Cell Sci (2003) 116 (7): 1367–1376.
Published: 1 April 2003
... by the expression of a constitutively active Rac mutant. Moreover,repression of Rac1 synthesis with siRNA amplifies the ephrinA1-induced inhibition of spreading. Finally, sphingosine-1-phosphate (S1P), a lipid mediator known to inhibit Rac activation in VSMCs amplifies the ephrinA1 effect. In conclusion, our...