... and the identity of the vesicles. We resolve the morphogenesis into vacuolization, vesiculation and maturation, and define the molecular characteristics and requirements of each step. Vacuolization is fueled by macropinocytosis and occurs by default if not blocked by high cell density or matrix proteins. Fine...

... of reactive oxygen species, but instead prevents acidification of the vacuole, perturbs metabolic regulation and promotes senescence. Central to the toxic gain-of-function seen with the SOD1 mutants examined was an inability to regulate amino acid biosynthesis. We also report that leucine supplementation...

...Yann Desfougères; Heinz Neumann; Andreas Mayer ABSTRACT Cells control the size of their compartments relative to cell volume, but there is also size control within each organelle. Yeast vacuoles neither burst nor do they collapse into a ruffled morphology, indicating that the volume...

...Henning Arlt; Fulvio Reggiori; Christian Ungermann ABSTRACT Endosomes are dynamic organelles that need to combine the ability to successfully deliver proteins and lipids to the lysosome-like vacuole, and recycle others to the Golgi or the plasma membrane. We now show that retromer, which...

... cellular self-digestion . Nature 451 , 1069 - 1075 . Nair U. , Cao Y. , Xie Z. , Klionsky D. J. ( 2010 ). Roles of the lipid-binding motifs of Atg18 and Atg21 in the cytoplasm to vacuole targeting pathway and autophagy . J. Biol. Chem. 285 , 11476 - 11488...

... mammalian endosome, the Rab GTPase Rab7 is a major regulator of fusion, whereas the homologous yeast protein Ypt7 seems to be restricted to the vacuole surface. Here, we present evidence that Ypt7 is recruited to and acts on late endosomes, where it affects multiple trafficking reactions. We show...

... role for Atg8 in vacuolar membrane dynamics. In the methylotrophic yeast Pichia pastoris , vacuoles were found to fuse to become a single spherical vacuole during adaptation from glucose- to methanol-containing medium. Atg8 is responsible for the vacuolar fusion in P. pastoris during this adaptation...

... from the endosomes to the vacuoles. These results suggest that a putative BLOC-1 complex in Arabidopsis might mediate the vacuolar degradative transport through direct interaction with SNX1 to regulate the homeostasis of PIN1 and PIN2, which is important for plant growth and development. To study...

...Sandra Codlin; Sara E. Mole Batten disease is characterised by lysosomal dysfunction. The most common type of the disease is caused by mutations in the membrane protein CLN3, whose function is unknown. We show that the fission yeast orthologue Btn1p, previously implicated in vacuole function...

...Sandra Codlin; Rebecca L. Haines; J. Jemima; E. Burden; Sara E. Mole btn1 , the Schizosaccharomyces pombe orthologue of the human Batten-disease gene CLN3 , is involved in vacuole pH homeostasis. We show that loss of btn1 also results in a defective cell wall marked by sensitivity to zymolyase, a β...

... for btn1 were viable but had enlarged and more alkaline vacuoles. Conversely overexpression of Btn1p reduced both vacuole diameter and pH. Thus Btn1p regulates vacuole homeostasis. The vacuolar defects of btn1 Δ cells were rescued by heterologous expression of CLN3, proving that Btn1p and CLN3...

...Daniel J. Klionsky Autophagy is a process in which cytosol and organelles are sequestered within double-membrane vesicles that deliver the contents to the lysosome/vacuole for degradation and recycling of the resulting macromolecules. It plays an important role in the cellular response to stress...

... © The Company of Biologists Limited 2003 2003 Multivesicular Vesicle Transport Vacuole Endosomes function to segregate endocytosed macromolecules destined to be degraded in lysosomal/vacuolar organelles from molecules that are either recycled back to the cell surface or routed...

...-mail: dbedwell@uab.edu ) 16 1 2003 © The Company of Biologists Limited 2003 2003 Yeast Pmr1p Ca 2+ homeostasis Vacuole Changes in the cytosolic Ca 2+ concentration are thought to participate in a variety of physiological processes in yeast, including cell-cycle control ( Hartley...

...Oliver Müller; Heinz Neumann; Martin J. Bayer; Andreas Mayer Vtc proteins have genetic and physical relations with the vacuolar H + -ATPase (V-ATPase), influence vacuolar H + uptake and,like the V-ATPase V 0 sectors, are important factors in vacuolar membrane fusion. Vacuoles from vtc1 Δ and vtc4...

... to the lumen of the vacuole. Sorting of M-C 6 -NBD-PC to the vacuole is blocked by energy-depletion and by null mutations in the VPS4 and VPS28 genes required for vesicular traffic from the pre-vacuolar compartment (PVC) to the vacuole. This sorting is not blocked by a temperature-sensitive mutation in SEC12...

.... Following glucose replenishment, FBPase is targeted from the cytosol to intermediate Vid(vacuole import and degradation) vesicles and then to the vacuole for degradation. Recently, several vid mutants that are unable to degrade FBPase in response to glucose were identified. Here, we present VID22 , a novel...

... sequence. The phosphorylation of these residues facilitates the ubiquitination and internalization of the permease. Following endocytosis, the permease is targeted to the lysosome/vacuole for proteolysis. We have shown that in casein kinase 1 (CK1)-deficient cells, the permease is poorly phosphorylated...

...Róża Kucharczyk; Andrzej M. Kierzek; P. P. Slonimski; Joanna Rytka Previously we have shown that the Saccharomyces cerevisiae CCZ1 ( YBR131w ) gene encodes a protein involved in protein trafficking. Deletion of this gene leads to fragmentation of the vacuole typical of the class B vps mutants...