... in its near native state. Quantitative cryo-SXT showed that Shigella fragments mitochondria and enabled visualization of X-ray dense structures (∼30 nm resolution) surrounding Shigella entrapped in septin cages. Using Airyscan confocal microscopy, we observed Lysine 63 (K63)-linked ubiquitin chains...

... on reversible post-translational modifications (PTMs) with ubiquitin (Ub) and ubiquitin-like (Ubl) proteins. The cycle of conjugation, recognition and removal of different Ub- and Ubl-modified states informs cellular protein stability and behavior at spatial and temporal resolution and is thus well suited...

... for formation of Dvl2 condensates during Wnt signalling. Ubiquitin Phase separation Dishevelled WWP2 Protein condensates Wnt signalling Department of Science & Technology, Government of India https://doi.org/10.13039/501100001409 EMR/2016/006928 Department of Biotechnology...

..., relieving inhibition of Smo. Smo then concentrates in cilia, becomes activated and activates downstream signaling. Loss of the ubiquitin E3 ligase Arih2 elevates basal Hedgehog signaling, elevates the cellular level of Smo and increases basal levels of ciliary Smo. Mice express two isoforms of Arih2...

... pathways, such as sonic hedgehog (SHH) signaling, during embryonic development. Our recent studies have shown that loss of GID ubiquitin ligase function results in aberrant AMP-activated protein kinase (AMPK) activation and elongated primary cilia, which suggests a functional connection to cilia. Here, we...

...Jianhui Li; Mark Hochstrasser ABSTRACT The proteasome is central to proteolysis by the ubiquitin-proteasome system under normal growth conditions but is itself degraded through macroautophagy under nutrient stress. A recently described AMP-activated protein kinase (AMPK)-regulated endosomal sorting...

...Thomas Tischer; Jing Yang; David Barford ABSTRACT The control of protein abundance is a fundamental regulatory mechanism during mitosis. The anaphase-promoting complex/cyclosome (APC/C) is the main protein ubiquitin ligase responsible for the temporal regulation of mitotic progression. It has been...

...-1-AP), p47 (15620-1-AP) antibodies are from Proteintech. Rabbit anti-p97 antibody for immunopurification and immunoblotting is from Bethyl (A300-589A), mouse FLAG-M2 antibody was from Sigma-Aldrich; mouse ubiquitin (FK2) antibody used for immunofluorescence was from EMD Millipore; ubiquitin antibody...

... of DNA double-strand breaks (DSBs). Here, we demonstrate that the SUMO-dependent recruitment of the deubiquitylating enzyme ataxin-3 to DSBs, unlike recruitment of the ubiquitin ligase RNF4, additionally depends on poly [ADP-ribose] polymerase 1 (PARP1)-mediated poly(ADP-ribosyl)ation (PARylation...

... distinct chaperone that forms a tripartite complex with PBAC1 and PBAC2 to promote assembly of the 20S proteasome α-ring in Arabidopsis . Arabidopsis Chaperone Core protease Degradation Evolution Proteasome Proteolysis Proteostasis Regulatory particle Ubiquitin National...

... for recruitment of Sla1 to the membrane. Interestingly, a Sla1 fragment containing just the third SH3 domain, which binds ubiquitin, and the SHD1 domain displayed broad surface localization, suggesting plasma membrane recruitment is mediated by interaction with both NPFxD-containing and ubiquitylated plasma...

... substantial progress in the field, a long-standing question remains: given that all ubiquitin units are identical, how do different elements of the sorting machinery recognize their specific cargoes? Our results indicate that the yeast Na + pump Ena1 is an epsin (Ent1 and Ent2 in yeast)-specific cargo...

...Annette Aichem; Marcus Groettrup ABSTRACT Human leukocyte antigen (HLA)-F adjacent transcript 10 (FAT10) also called ubiquitin D (UBD) is a member of the ubiquitin-like modifier (ULM) family. The FAT10 gene is localized in the MHC class I locus and FAT10 protein expression is mainly restricted...

... throughout the cytosol and serve as antigen storage sites synchronising DC maturation and antigen presentation. Maturation of DCs is accompanied by the induction of the ubiquitin-like modifier FAT10 (also known as UBD), which localises to aggresomes, structures that are similar to DALIS. FAT10 is conjugated...

... expressed at endogenous levels. We also show that it can be applied to decipher the topology of ubiquitin conjugates. Moreover, we assembled a genome-wide collection of yeast strains ready to investigate the ubiquitylation of proteins with this new assay. This resource will facilitate the analysis of local...

...-43-associated neurotoxicity. We demonstrate that Tnks-1/2 interacts with TDP-43 via a newly defined tankyrase-binding domain. Upon investigating the functional effect, we find that interaction with Tnks-1/2 inhibits the ubiquitination and proteasomal turnover of TDP-43, leading to its stabilization...

... mediates the budding of intralumenal vesicles (ILVs). In Saccharomyces cerevisiae , ESCRT-III activity at endosomes is regulated through an unknown mechanism by Doa4, an ubiquitin hydrolase that deubiquitylates transmembrane proteins sorted into ILVs. We report that the non-catalytic N-terminus of Doa4...

...Yaya Wang; Diana Argiles-Castillo; Emma I. Kane; Anning Zhou; Donald E. Spratt ABSTRACT Homologous to E6AP C-terminus (HECT) E3 ubiquitin ligases play a critical role in various cellular pathways, including but not limited to protein trafficking, subcellular localization, innate immune response...

... © 2020. Published by The Company of Biologists Ltd 2020 http://www.biologists.com/user-licence-1-1/ Summary: USP16 is actively excluded from the nucleus and this DUB might indirectly regulate DSB repair. Deubiquitylase Ubiquitin USP16 UBP-M H2AK119 Nuclear export Cell...

... of the cyclin E N-terminus occurs in some cancers and is associated with increased cyclin E–Cdk2 activity and poor clinical prognosis. Cyclin E levels are tightly regulated and controlled in part through ubiquitin-mediated degradation initiated by one of two E3 ligases, Cul1 and Cul3. Cul1 ubiquitylates...