... of K8 in the lung has not been reported in mutant transgenic mouse models. Here, we investigated the susceptibility of two different transgenic mice expressing K8 Gly62–Cys (Gly62 replaced with Cys) or Ser74–Ala (Ser74 replaced with Ala) to lung injury. The mutant transgenic mice were highly susceptible...

... of α7β1 integrin contributes to MDC1A disease progression. To investigate whether restoring α7 integrin expression can alleviate the myopathic phenotype observed in MDC1A, we produced transgenic mice that overexpressed the α7 integrin in the skeletal muscle of the dy W−/− mouse model of MDC1A. Enhanced...

... Council on Animal Care. The different vimentin constructs used have been previously described ( Schietke et al., 2006 ). Transgenic mice were generated at the Karolinska Institut, Center for Transgene Technologies, (Stockholm, Sweden) in a CBA background. Founder animals were backcrossed to C57Bl/6. Mice...

... and MEF2D), the ability of cells to differentiate may have been confounded by genetic redundancy. To eliminate this variable, the effect of a dominant-negative MEF2 mutant (MEF2C/EnR) during cardiomyogenesis was examined in transgenic mice and P19 cells. Targeting the expression of MEF2C/EnR...

.... Cytoskeleton Transgenic mice Extracellular matrix Nerve regeneration Adhesion © The Company of Biologists Limited 2006 2006 14 7 2006 * Author for correspondence (e-mail: previtali.stefano@hsr.it ) References Akassoglou, K., Yu, W. M., Akpinar, P. and Strickland, S. ( 2002...

..., 1996 ). α-dystroglycan binds to laminin in the extracellular matrix. Overall, the DGC links the muscle cell cytoskeleton to the extracellular matrix and maintains sarcolemmal integrity. α7 integrin Dystrophin Transgenic mice Double knockout Skeletal muscle Muscular dystrophy ©...

... function of Cx43 during parturition in the living animal and suggest further strategies to investigate the role of connexins in uterine contractility in transgenic mice. * Author for correspondence (e-mail: genetik@uni-bonn.de ) 18 1 2006 © The Company of Biologists Limited 2006 2006...

... growth factor-β propeptide and the latent transforming growth factor-β-binding protein. We studied the role of latent transforming growth factor-β-binding protein in modulating transforming growth factor-β availability by generating transgenic mice that express a truncated form of latent transforming...

... the mechanism linking Cdk5 to cytoskeletal reorganization and migration. Cdk5 was overexpressed in the corneal epithelium of transgenic mice under control of the ALDH3 promoter. Elevated Cdk5 expression retarded corneal debridement wound closure in these animals and suppressed remodeling of the actin...

.... Osteoblast differentiation was delayed in calvaria cells isolated from transgenic mice. Likewise, the number of osteoblast precursors in bone marrow stromal cells from OG2-NcadΔC mice was decreased compared with wild-type cultures, whereas the number of adipogenic precursors was increased. In vitro...

... in vitro sensitivity, trypsin immunolabeling of cell bodies was stronger in transgenic mice. These results show a novel mechanism to regulate the amount of neurofilaments when they abnormally aggregate. Neurofilaments Aggregation Transgenic mice Trypsin proteolysis Neurofilaments...

... of transgenic mice were found to maintain transgene expression status in culture. However, similar cultures prepared from nontransgenic mice or rats show uniform expression after transient transfection of Mlc3f constructs, suggesting that the mechanism responsible for differential expression of the transgene...

...-1 indicates that the two receptors are induced in the skin endothelium of transgenic mice suggesting that both are involved in mediating the effect of overexpressed PlGF. The role of PlGF as an angiogenic factor has been debated for a long time. In this study we investigated the in vivo angiogenic...

...Xinran Liu; Ting-Huai Wu; Sally Stowe; Atsuko Matsushita; Kentaro Arikawa; Muna I. Naash; David S. Williams ABSTRACT Retinitis pigmentosa is a heterogeneous group of inherited retinal disorders in which the photoreceptor cells degenerate. A line of transgenic mice expresses a mutant opsin gene...

... replaced by the Escherichia coli lacZ gene, the resulting construct showed no expression in transgenic mice. This suggests that 5′ and 3′ flanking sequences, in the absence of intragenic sequences, are not sufficient for K8 expression and that important control elements are located in the body of the K8...