... provided from a sample and one must be careful about the possibility of artifacts produced during sample preparation ( Table S1 ). There are two main types of light microscopy modalities routinely used to study CME, total internal reflection fluorescence microscopy (TIRF-M) and confocal microscopy...

... TIRF objective (Olympus UApo N; numerical aperture, NA=1.49) was used for measurements of local Ca 2+ signals by using total internal reflection fluorescence microscopy (TIRFM). Excitation (488 nm) was provided by a diode-pumped solid-state laser (150 mW, iLas Laser System, Cairn) and a band-pass...

... diffusion out of plasma membrane MHC-I patches directly influences the size and persistence of the patches, which could extensively impact on CTL recognition of foreign peptides. Major histocompatibility complex class I MHC-I Total internal reflection fluorescence microscopy TIRFM Fluorescence...

... . Axelrod D. ( 1989 ). Total internal-reflection fluorescence microscopy . Methods Cell Biol. 30 , 245 - 270 . Axelrod D. ( 2003 ). Total internal reflection fluorescence microscopy in cell biology . Biophotonics B 361 , 1 - 33 . Axelrod D. ( 2008 ). Total internal...

... 2009 EGF receptor Total internal reflection fluorescence microscopy Endocytosis The epidermal growth factor receptor (EGFR) is a frequently studied canonical member of the receptor tyrosine kinase superfamily ( Atalay et al., 2003 ). EGFR family members play roles in important...

... Limited 2006 2006 Exocytosis Rab3A Rab27A Small interfering RNA Total internal reflection fluorescence microscopy The release of neurotransmitters and peptide hormones involves exocytotic fusion of secretory vesicles with the plasma membrane. Although the precise mechanism that allows...