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Keywords: Tetrahymena
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Journal Articles
J Cell Sci (2023) 136 (22): jcs261511.
Published: 27 November 2023
... osmoregulatory roles across diverse eukaryotic lineages, but probing the mechanisms underlying their structure and function is hampered by the limited tools available for in vivo analysis. In the experimentally tractable ciliate Tetrahymena thermophila , we describe four proteins that, as endogenously tagged...
Includes: Supplementary data
Journal Articles
In collection:
Cilia and Flagella
J Cell Sci (2020) 133 (11): jcs240838.
Published: 4 June 2020
... associate with BBs and function in BB assembly, maturation and stability. Human POC5 (hPOC5) is a highly conserved centrin-binding protein that binds centrins through Sfi1p-like repeats and is required for building full-length, mature centrioles. Here, we use the BB-rich cytoskeleton of Tetrahymena...
Includes: Supplementary data
Journal Articles
J Cell Sci (2017) 130 (2): 480–489.
Published: 15 January 2017
... in the regulation of chromatin structure in the ciliated protozoan Tetrahymena thermophila . Jub6p forms sodium dodecyl sulfate (SDS)-resistant aggregates when it is ectopically expressed in vegetative cells and binds to RNA in vitro . Jub6p is a heterochromatin component and is important for the formation...
Includes: Supplementary data
Journal Articles
J Cell Sci (2012) 125 (2): 516–527.
Published: 15 January 2012
... and genomic stability and relies on the correct establishment of the cell division axis, which is under the control of the cell's environment and its intrinsic polarity. The ciliate Tetrahymena thermophila possesses a permanent anterior–posterior axis, left–right asymmetry and divides symmetrically...
Includes: Supplementary data
Journal Articles
Journal Articles
J Cell Sci (1999) 112 (21): 3619–3626.
Published: 1 November 1999
...Kohsuke Gonda; Mariko Katoh; Kazuko Hanyu; Yoshio Watanabe; Osamu Numata ABSTRACT Tetrahymena p85 differs in mobility in two-dimensional SDS-polyacrylamide gel electrophoresis between wild-type and temperature-sensitive cell-division-arrest mutant cdaA1 cell extracts, and is localized...
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Journal Articles
J Cell Sci (1991) 1991 (Supplement_14): 117–120.
Published: 1 January 1991
...E. M. CROSSLEY; S. C. HYMAN; C. WELLS Summary Axonemal dynein from Tetrahymena cilia can be separated on a sucrose gradient into two fractions, at least one of which appears to be polymorphic. We have been using immuno-electron microscopy in order to try and locate the different types of dynein...
Journal Articles
J Cell Sci (1990) 96 (2): 293–302.
Published: 1 June 1990
...Jerry E. Honts; Norman E. Williams ABSTRACT The cortex of the ciliated protozoan Tetrahymena contains a number of fibrous elements, including a network of filaments that pervades the feeding organelle of this organism. The cluster of polypeptides (79–89K; K=10 3 M T ) in Tetrahymena pyriformis GL-C...
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Journal Articles
J Cell Sci (1989) 92 (2): 319–324.
Published: 1 February 1989
...Per Hellung-Larsen; Anders Poul Andersen ABSTRACT Cultures of Tetrahymena pyriformis, T. hegewishi and T. malaccensis have been studied with regard to control of cell volume and cellular dry weight. Cell volume was measured on cells suspended in 0-9% sodium chloride + 0 ·1% sodium azide using...
Journal Articles
J Cell Sci (1987) 87 (3): 457–463.
Published: 1 April 1987
...N. E. Williams; J. E. Honts; R. F. Jaeckel-Williams ABSTRACT Antisera have been raised in rabbits against three high molecular weight proteins that are present in Triton X-100-insoluble residues of Tetrahymena pyriformis GL cells. These proteins, called A, B and C, have apparent molecular weights...
Journal Articles
J Cell Sci (1986) 83 (1): 155–164.
Published: 1 July 1986
...Joachim Roth; Günter Cleffmann ABSTRACT By combining cytophotometry with autoradiography, five stages of macronuclear anlagen can be discriminated by their DNA content until the end of the first cell cycle after conjugation in Tetrahymena . DNA increases from 2 C to about 32 C . Each S -phase...
Journal Articles
J Cell Sci (1985) 78 (1): 49–52.
Published: 1 October 1985
...Nita J. Maihle; Birgit H. Satir ABSTRACT The ciliated protozoon Tetrahymena thermophila contains membrane-bounded secretory organelles termed mucocysts, the release of which has previously been characterized ultrastructurally as a model system for the events occurring during membrane fusion...
Journal Articles
J Cell Sci (1985) 78 (1): 23–48.
Published: 1 October 1985
...Donna M. Pesciotta; Birgit H. Satir ABSTRACT Culturing the ciliate Tetrahymena thermophila in Cerophyl R has provided an opportunity for studying the assembly and/or synthesis of the intramembrane particle array, the rosette, which marks the site of exocytosis in these cells. Cultures grown...
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Journal Articles
J Cell Sci (1985) 73 (1): 279–297.
Published: 1 February 1985
...E. Jane Mitchell; Arthur M. Zimmerman ABSTRACT A protein from an ATP extract prepared from an acetone powder of Tetrahymena pyrifonnis GL was identified as actin. The protein migrated slightly behind muscle actin on sodium dodecyl sulphate (SDS)/10% polyacrylamide gels (SDS/PAGE) with an apparent...
Journal Articles
J Cell Sci (1985) 73 (1): 299–310.
Published: 1 February 1985
... arms were normally preserved in parallel preparations of Chlamydomonas axonemes. Isolated dynein from the ciliated protozoon Tetrahymena bound to Marsilea axonemes at the site normally occupied by the outer arm. Dynein binding was partially reversed by ATP as judged by both electron microscopy...