...Ru Jia; Yongping Chai; Chao Xie; Gai Liu; Zhiwen Zhu; Kaiyao Huang; Wei Li; Guangshuo Ou ABSTRACT Perturbation of spectrin-based membrane mechanics causes hereditary elliptocytosis and spinocerebellar ataxia, but the underlying cellular basis of pathogenesis remains unclear. Here, we introduced...

... of the spectrin–F-actin and α-actinin–F-actin networks at the membrane. These rearranged F-actin networks appear to be less able to support mechanical load and resilience, leading to an overall change in tissue mechanical properties. This is the first in vivo evidence that a Tpm protein is essential for cell...

... remains unclear. Here, we examine the hierarchy of events during cytoskeletal polarisation in Drosophila melanogaster epithelia. Core apical-basal polarity determinants polarise the spectrin cytoskeleton to recruit the microtubule-binding proteins Patronin (CAMSAP1, CAMSAP2 and CAMPSAP3 in humans...

... the distance between them. Relative to other probes, it is physically smaller and exhibits a greater dynamic range and sensitivity and expresses well. For in vivo testing, we measured stress gradients in time and space in non-erythroid spectrin in several different cell types and found that spectrin is under...

... -spectrin (β H ) and the multivesicular body (MVB) in Drosophila . AnxB9 binds to a subset of β H spliceoforms, and loss of AnxB9 results in an increase in basolateral β H and its appearance on cytoplasmic vesicles that overlap with the MVB markers Hrs, Vps16 and EPS15. Similar colocalizations are seen when...

.... Roughest and the cadherin-based zonula adherens (ZA) are interdependent and both are modulated by the apical polarity determinant, Crumbs. Here we describe a novel relationship between the Crumbs partner β Heavy -spectrin (β H ), the ZA and Roughest. Ectopic expression of the C-terminal segment 33 of β H...

...Qiaozhen Kang; Ting Wang; Huizheng Zhang; Narla Mohandas; Xiuli An The archetypal membrane skeleton is that of the erythrocyte, consisting predominantly of spectrin, actin, ankyrin R and protein 4.1R. The presence in the Golgi of a membrane skeleton with a similar structure has been inferred, based...

..., as well as increased plasma creatine kinase levels. Costameres at the sarcolemma of keratin-19-null muscle, visualized with antibodies against spectrin or dystrophin, were disrupted and the sarcolemma was separated from adjacent myofibrils by a large gap in which mitochondria accumulated. The costameric...

..., and a decrease in epithelial cell height, without effects on localisation of tight junction or adherens junction proteins, or any apparent changes in cell-cell adhesion. Instead, distribution of αII-spectrin on lateral membranes is disrupted upon reduction of Tmod3 levels, suggesting that loss of Tmod3 function...

...Matthew D. Phillips; Claire M. Thomas An apical brush border is a characteristic of many mature epithelia. This dynamic structure consists of dense microvilli supported by F-actin bundles that protrude into the apical cytoplasm, where they are crosslinked by spectrin and myosin II to form...

...Katarzyna Bialkowska; Takaomi C. Saido; Joan E. B. Fox In this study, we used cultured cells spreading on β3 integrin substrates to examine the possibility that spectrin is involved in signal transduction. Spectrin clustered with specialized calpain-induced β3 integrin signaling complexes...

... polarized membrane domain. The spectrin-based membrane skeleton is thought to contribute to such stabilization by increasing the half-life of many proteins at the cell surface. Genetic evidence is consistent with a negative role for Drosophila β Heavy -spectrin in endocytosis, but the inhibitory mechanism...

...Jun Fan; Kenneth A. Beck Expression of a dominant negative fragment of the spectrin family member Syne-1 causes an accumulation of binucleate cells, suggesting a role for this protein in cytokinesis. An association of this fragment with the C-terminal tail domain of the kinesin II subunit KIF3B...

... the fact that they share features with both the spectrin and plakin superfamilies. These genes produce a variety of large proteins, up to almost 9000 residues long, which can potentially extend 0.4 μm across a cell. Spectraplakins can interact with all three elements of the cytoskeleton: actin...

... of the Na,K-ATPase colocalize with β-spectrin and ankyrin 3 in costameres, structures at the sarcolemma that lie over Z and M-lines and in longitudinal strands. In immunoprecipitates, the α1 and α2 subunits of the Na,K-ATPase as well as ankyrin 3 associate with β-spectrin/α-fodrin heteromers and with a pool...

..., water-soluble dyes into the culture medium, protein markers associated with this type of vesicles have not yet been well defined. Here, we report that human spectrin SH3 domain binding protein 1, or Hssh3bp1, associates with macropinosomes in NIH 3T3 fibroblasts. Hssh3bp1 macropinosomes...

...Nandini V. L. Hayes; Catherine Scott; Egidius Heerkens; Vasken Ohanian; Alison M. Maggs; Jennifer C. Pinder; Ekaterini Kordeli; Anthony J. Baines ABSTRACT It is established that variations in the structure and activities of βI spectrin are mediated by differential mRNA splicing. The two βI spectrin...

... myogenic regulator, a yeast two-hybrid screen was employed to identify cytoplasmic-associated MLP binding partners. From this screen, the cytoskeletal protein βI-spectrin was isolated. Protein interaction assays demonstrate that MLP and βI-spectrin associate with one another in vivo as well as when tested...

...Claire M. Thomas; Janice A. Williams ABSTRACT The origin of epithelial cell polarity during development is a fundamental problem in cell biology. Central to this process is the establishment of asymmetric membrane domains that will ultimately form the apical and basolateral surfaces. The spectrin...

...Rosa Carotenuto; Giovanna Maturi; Vincenzo Infante; Teresa Capriglione; Tamara C. Petrucci; Chiara Campanella ABSTRACT Cytoskeletal proteins such as actin and myosin are important constituents of the nucleoplasm. Spectrin is an actin binding protein typically related to plasma membrane; recently...