... confined but contribute to coherent chromatin motion across the entire nucleus. However, the regulation of dynamics, nuclear orientation and compaction of subregions along a single chromosome are not well-understood. We used CRISPR-based real-time single-particle tracking and polymer models to characterize...

.... Published by The Company of Biologists Ltd 2015 Summary: The principal experimental and analytical requirements to visualize and trace single endogenous mRNA particles in living cells are discussed, and the most recent quantitative results are highlighted. Single-particle tracking RNA...

... with membrane-spanning proteins that communicate with obligate cytoplasmic proteins. Transient anchorage of crosslinked proteins on the cell surface was previously characterized by single-particle tracking, and temporary association with the actin cytoskeleton was hypothesized to cause regulated anchorage...