... or obsolete secretory granules directly fuse with lysosomes to yield crinosomes, in which the delivered secretory material is degraded. Crinophagy maintains the proper intracellular pool of secretory granules, and it is enhanced when secretory material accumulates because of compromised secretion. Recent...

.../0166-6851(85)90059-3 Giuliani , F. , Grieve , A. and Rabouille , C. ( 2011 ). Unconventional secretion: a stress on GRASP . Curr. Opin. Cell Biol. 23 , 498 - 504 . 10.1016/j.ceb.2011.04.005 Glushakova , S. , Busse , B. L. , Garten , M. , Beck , J. R...

...Franziska Lehne; Sven Bogdan ABSTRACT Exocytosis is a fundamental cellular process by which cells secrete cargos from their apical membrane into the extracellular lumen. Cargo release proceeds in sequential steps that depend on coordinated assembly and organization of an actin cytoskeletal network...

... dissociation of the enzymes from mannose 6-P receptors, allowing for their recycling back to the trans /TGN ( Griffiths et al., 1988 ). Deficiency of N-acetylglucosamine 1-phosphotransferase leads to the lysosomal storage disorder mucolipidosis type 2 ( Kornfeld and Mellman, 1989 ), causes secretion...

... docking, but also mistargeting of SNARE protein syntaxin1 and an abnormally dense submembrane F-actin network. Here, we tested the contribution of both these phenomena to docking and secretion defects in MUNC18-1-deficient MCCs. We show that an abnormal F-actin network and syntaxin1 targeting defects...

...Camille Lenzi; Jennifer Stevens; Daniel Osborn; Matthew J. Hannah; Ruben Bierings; Tom Carter ABSTRACT Elevations of intracellular free Ca 2+ concentration ([Ca 2+ ] i ) are a potent trigger for Weibel–Palade body (WPB) exocytosis and secretion of von Willebrand factor (VWF) from endothelial cells...

...Jiaxin Sun; Shiyan Yu; Xiao Zhang; Catherine Capac; Onyedikachi Aligbe; Timothy Daudelin; Edward M. Bonder; Nan Gao ABSTRACT Wntless (Wls) transports Wnt molecules for secretion; however, the cellular mechanism underlying the initial assembly of Wnt secretory vesicles is still not fully defined...

...Duy T. Tran; Kelly G. Ten Hagen ABSTRACT Real-time imaging of regulated exocytosis in secreting organs can provide unprecedented temporal and spatial detail. Here, we highlight recent advances in 3D time-lapse imaging in Drosophila salivary glands at single-granule resolution. Using fluorescently...

...Charles Campbell; Shawn Beug; Philip E. B. Nickerson; Jimmy Peng; Chantal Mazerolle; Erin A. Bassett; Randy Ringuette; Fadumo A. Jama; Carlos Morales; Annabel Christ; Valerie A. Wallace ABSTRACT Sonic Hedgehog (Shh) is a secreted morphogen that is an essential regulator of patterning and growth...

... machinery in ERES, and a capture model, where cargo freely diffuses into ERES where it is captured by the Sar1 and COPII machinery. Using the novel secretion inhibitor FLI-06, we show that recruitment of the cargo VSVG to ERES is an active process upstream of Sar1 and COPII. Applying FLI-06 before...

... and specialised mechanisms required to facilitate focal signalling and polarised secretion in immune cells. In this Cell Science at a Glance article and the accompanying poster, we illustrate the different steps that reveal the specialised mechanisms used to focus secretion at the CTL immune synapse and allow...

... (NGF) acting through the TrkA neurotrophin receptor (also known as NTRK1) regulates many of these events. However, the molecular mechanisms responsible for NGF-regulated secretion are not completely understood. Here, we describe a new signaling pathway involving TrkA, ARMS (also known as Kidins220...

..., COMP also fulfills an important intracellular function in assisting efficient secretion of collagens, which were retained in the endoplasmic reticulum of COMP-null fibroblasts. Accordingly, COMP-null mice showed severely attenuated fibrotic responses in skin. Collagen secretion was fully restored...

...Young Hun Kim; Man Sup Kwak; Jun Bae Park; Shin-Ae Lee; Ji Eun Choi; Hyun-Soo Cho; Jeon-Soo Shin ABSTRACT HMGB1 protein is a delayed mediator of sepsis that is secreted to the extracellular milieu in response to various stimulants, inducing a pro-inflammatory response. HMGB1 is devoid...

... certain tail-anchored proteins is directly localized to the surface of the endoplasmic reticulum, facilitating the insertion of newly synthesized proteins into the membrane. Endoplasmic reticulum mRNA localization Secretion Tail-anchored protein One major mechanism that directs proteins...

... for the Aβ-lowering effect of CALHM1. Aβ level reduction by CALHM1 could be explained by an increase in extracellular Aβ degradation by insulin-degrading enzyme (IDE), extracellular secretion of which was strongly potentiated by CALHM1 activation. Importantly, Calhm1 knockout in mice reduced IDE enzymatic...

...Na-Ryum Bin; Chang Hun Jung; Byungjin Kim; Prashanth Chandrasegram; Ekaterina Turlova; Dan Zhu; Herbert Y. Gaisano; Hong-Shuo Sun; Shuzo Sugita ABSTRACT Understanding how Munc18 proteins govern exocytosis is crucial because mutations of this protein cause severe secretion deficits in neuronal...

... attributed. Golgi Golgin Membrane traffic Secretion ERGIC The Golgi complex lies at the heart of the secretory pathway where it acts as a processing factory and sorting hub for cargo proteins and lipids ( Rothman, 1981 ). The vertebrate Golgi complex comprises stacked cisternae...

... and crosslinking, and myosin II activity, to contract actin coats and drive secretion. Total RNA was isolated from 10 6 ATII cells directly after isolation or following 48 h of culture in MucilAir medium with an RNeasy MiniKit (Qiagen, Hilden, Germany). Reverse transcription was performed on 0.8 µg to 1.3 µg...

... VI (COL6), and postulated that ANXA2 directs bronchial epithelial cell secretion of COL6, an unusually large multimeric protein. COL6 serves to anchor cells to basement membranes and, unlike other collagens, undergoes multimerization prior to secretion. Here, we show that AnxA2 −/− mice have reduced...