... that Snap-25 -null mutant (SNAP-25 KO) neurons, previously shown to degenerate after 4 days in vitro (DIV), contain fewer DCVs and have reduced DCV fusion probability in surviving neurons at DIV14. At DIV3, before degeneration, SNAP-25 KO neurons show normal DCV fusion, but one day later fusion...

...Aram Megighian; Michele Scorzeto; Damiano Zanini; Sergio Pantano; Michela Rigoni; Clara Benna; Ornella Rossetto; Cesare Montecucco; Mauro Zordan An analysis of SNAP-25 isoform sequences indicates that there is a highly conserved arginine residue (198 in vertebrates, 206 in the genus Drosophila...

... syntaxin 4 and cytoplasmic murine CENPF, a protein previously demonstrated to associate with the microtubule network and SNAP-25. The binding domain for syntaxin 4 in CENPF was defined by yeast two-hybrid assay and co-immunoprecipitation. Confocal analyses in cell culture reveal a high degree...

...Helmut Kubista; Hannah Edelbauer; Stefan Boehm The core complex, formed by the SNARE proteins synaptobrevin 2, syntaxin 1 and SNAP-25, is an important component of the synaptic fusion machinery and shows remarkable in vitro stability, as exemplified by its SDS-resistance. In western blots...

...Darshan K. Koticha; Ellen E. McCarthy; Giulia Baldini SNAP-25 is an integral protein of the plasma membrane involved in neurotransmission and hormone secretion. The cysteine-rich domain of SNAP-25 is essential for membrane binding and plasma-membrane targeting. However, this domain is not required...

...Gary W. Lawrence; J. Oliver Dolly Ca 2+ -triggered catecholamine exocytosis from chromaffin cells involves SNAP-25, synaptobrevin and syntaxin (known as SNAREs). Synaptotagmin I has been implicated as the Ca 2+ -sensor because it binds Ca 2+ , and this enhances its binding to syntaxin, SNAP-25...

... and in which SNAP-25 and syntaxin predominate over synaptobrevin. Their formation increased upon Ca 2+ -stimulated exocytosis; notably, the 2D protocol proved much superior to 1D SDS-PAGE for the detection of large complexes and revealed that for forms with relative molecular mass greater than 100,000...