... initiates mitophagosome formation remains unknown. The Rab7 GTPase (herein referring to Rab7a) is a key initiator of mitophagosome formation, and Ser-72 phosphorylation of Rab7 is important for this process. We have previously identified LRRK1 as a protein kinase responsible for Rab7 Ser-72 phosphorylation...

.... Proteome analysis by complex mass-spectrometry (MS/MS) revealed a dramatically altered SUMO-proteome (SUMOylome) in S. Typhimurium-infected cells. RAB7, a component of VTP, was key among several crucial proteins identified in our study. Detailed MS/MS assays, in vitro SUMOylation assays and structural...

... of Biologists Ltd 2018 http://www.biologists.com/user-licence-1-1/ Summary: Sequestration of LTβR in intraluminal vesicles of endosomes upon depletion of HOPS components or Rab7 precludes NF-κB signaling, whereas LTβR accumulation on the outer endosomal membrane after ESCRT depletion promotes...

... observe changes in the morphology of lysosomes (using the protein Lamp1 as a marker) after TBC1D5 knockdown in line with changes elicited by knockdown of Rab7a itself (see Fig. S4 ). Studies in yeast have shown that the membrane-associated SNX-BAR dimer of Vps5p-Vps17p can displace yeast Rab7 (Ypt7p...

...-dependent manner, whether starvation also regulates autophagosome or autolysosome maturation was unclear. In the present study, we succeeded in demonstrating that starvation activates autolysosome maturation in mammalian cells. We found that knockout (KO) of Rab7 (herein referring to the Rab7a isoform...

.... ( 2001 ). Rab-interacting lysosomal protein (RILP): the Rab7 effector required for transport to lysosomes . EMBO J.   20 , 683 - 693 . 10.1093/emboj/20.4.683 Ceresa , B. P. and Peterson , J. L. ( 2014 ). Cell and molecular biology of epidermal growth factor receptor . Int. Rev. Cell...

... membrane proteins. Dysfunction of this complex has been linked to Alzheimer's disease and Parkinson's disease. The recruitment of retromer to endosomes is regulated by Rab7 (also known as RAB7A) to coordinate endosome-to-TGN trafficking of cargo receptor complexes. Rab7 is also required for the degradation...

..., inhibited cellular response to SDF-1, caused accumulation and aggregation of multivesicular bodies (MVBs) in the cytoplasm, and promoted CXCR4 sorting into MVBs in a manner depending on binding to AIP4. In response to SDF-1, MIM also bound transiently to the small GTPase Rab5 at 5 min and to Rab7 at 30 min...

... were unaffected despite elevated Rab5-GTP levels. Strikingly, a large increase in Rab7-GTP levels, an accumulation of enlarged late endosomes, and decreased EGFR degradation were observed in Vps34-deficient cells. The hyperactivation of Rab7 in Vps34-deficient cells stemmed from the failure to recruit...

... of endosomal Rab7 inhibited, and lowering membrane cholesterol levels favored STxB–saporin translocation. Interestingly, experiments with reducible and non-reducible linker-arm–STxB conjugates led to the conclusion that after translocation, STxB remains associated with the cytosolic membrane leaflet...

... by the formation of numerous tubules that are dependent upon the increase in Rab7 activity observed in the HBV‐expressing HepG2.2.15 cells compared to HepG2 cells. Interestingly, transfection‐based expression of the five individual viral proteins indicated that the precore protein, which is a precursor of HBeAg...

...Maria De Luca; Laura Cogli; Cinzia Progida; Veronica Nisi; Roberta Pascolutti; Sara Sigismund; Pier Paolo Di Fiore; Cecilia Bucci ABSTRACT Rab-interacting lysosomal protein (RILP) is a downstream effector of the Rab7 GTPase. GTP-bound Rab7 recruits RILP to endosomal membranes and, together...

... over very long distances before they tether to initiate contact. Here, we describe that lysosomal tethering and transport are combined processes co-regulated by one multi-protein complex: RAB7–RILP–ORP1L. We show that RILP directly and concomitantly binds the tethering HOPS complex and the p150 Glued...

...Jahan Yousefian; Tobias Troost; Ferdi Grawe; Takeshi Sasamura; Mark Fortini; Thomas Klein Summary The small GTPases Rab5 and Rab7 are important organisers of endosome formation and maturation. In addition, they orchestrate the trafficking of cargo through the endosomal pathway. A crucial event...

... protein receptors (SNAREs) and can tether membranes. Owing to the presence of specific subunits, CORVET is a Rab5 effector complex, whereas HOPS can bind efficiently to late endosomes and lysosomes through Rab7. Based on the recently described overall structure of the HOPS complex and a number of in vivo...

... temporally upregulate their secretion of Flii. Using siRNA, and wild-type and mutant proteins, we show that Flii is secreted by means of a late endosomal/lysosomal pathway that is regulated by Rab7 and Stx11. Flii contains 11 leucine-rich repeat domains in its N-terminus that have nearly 50% similarity...

...Cinzia Progida; Laura Cogli; Francesco Piro; Azzurra De Luca; Oddmund Bakke; Cecilia Bucci Rab7b is a recently identified member of the Rab GTPase protein family and has high similarity to Rab7. It has been reported that Rab7b is lysosome associated, that it is involved in monocytic differentiation...

... cells. Lysosomes were located more peripherally in Rab7-shRNA-expressing cells and these cells were more invasive than control cells. Overexpression of the Rab7 effector protein, RILP, resulted in a juxtanuclear location of lysosomes and reduced HGF-induced invasion. Together, these results suggest...

... PtdIns3 P -binding PX domains, the cargo-selective VPS35/29/26 complex has no lipid-binding domains and its recruitment to the endosomal membrane remains mechanistically uncharacterised. In this study we show that the VPS35/29/26 complex interacts with the small GTPase Rab7 and requires Rab7 for its...

... sorting of ubiquitylated receptors into intralumenal vesicles (ILVs) of multivesicular endosomes (MVEs) that move along microtubules to fuse with perinuclear lysosomes. The Rab7-interacting lysosomal protein RILP is interesting in this context as it interacts with Vps22 (also known as EAP30) and Vps36...