... increase in cellular cAMP and consequent responses in the cytoplasm required for cell–cell fusion. Here, we identify a new participant in ciliary signaling, Gamete-Specific Protein Kinase (GSPK). GSPK is essential for the adhesion-induced cAMP increase and for rapid gamete fusion. The protein...

... regulator of new end growth and demonstrated that Fic1, a cytokinetic factor, is required for normal polarized growth at new ends. Here, we report that Fic1 is phosphorylated on two C-terminal residues, which are each targeted by multiple protein kinases. Endogenously expressed Fic1 phosphomutants cannot...

...Margot Riggi; Beata Kusmider; Robbie Loewith ABSTRACT Target of rapamycin (TOR) is a serine/threonine protein kinase conserved in most eukaryote organisms. TOR assembles into two multiprotein complexes (TORC1 and TORC2), which function as regulators of cellular growth and homeostasis by serving...

... by The Company of Biologists Ltd 2015 PKM2 Pyruvate kinase M2 Metabolism reprogramming Protein kinase Gene transcription Pyruvate kinase (PK) regulates the final rate‐limiting step of glycolysis by catalyzing the transfer of a phosphate group from phosphoenolpyruvate (PEP) to ADP to produce...

...Ivo Zemp; Franziska Wandrey; Sanjana Rao; Caroline Ashiono; Emanuel Wyler; Christian Montellese; Ulrike Kutay ABSTRACT Biogenesis of 40S pre-ribosomal subunits requires many trans-acting factors, among them several protein kinases. In this study, we show that the human casein kinase 1 (CK1...

... ( nils.johnsson@uni-ulm.de ) 9 9 2010 © 2011. 2011 Constraint interaction network Protein–protein interaction Organelle inheritance Polarized growth Protein kinase Protein phosphatase Systems biology Inactivation of proteins that participate in more than one cellular process leads...

...Barry J. Collins; Maria Deak; Vicky Murray-Tait; Kate G. Storey; Dario R. Alessi AGC kinases are mediators of signalling responses stimulated by agonists and are activated following phosphorylation at their T-loop residue by the 3-phosphoinositide-dependent protein kinase-1 (PDK1). Agonists...

...Krysten J. Palmer; Joanne E. Konkel; David J. Stephens The export of secretory cargo from the endoplasmic reticulum is mediated by the COPII complex. In common with other aspects of intracellular transport, this step is regulated by protein kinase signalling. Recruitment of the COPII complex...

...Jennifer L. Morrell; Connie B. Nichols; Kathleen L. Gould Two relatives of the GIN4 protein kinase family, Cdr1p and Cdr2p, exist in the yeast Schizosaccharomyces pombe. Although in Saccharomyces cerevisiae GIN4-related kinases influence septin ring organization and septin rings influence...

...Christine Latif; Nicole R. den Elzen; Matthew J. O'Connell The G2 DNA damage checkpoint prevents mitotic entry in the presence of DNA damage. This requires the activation of the phosphoinositide-3-kinase-related protein kinases ATR and ATM in human cells and the ATR homologue Rad3 in the fission...

...Stephan Bek; Rolf Kemler β-Catenin is a multi-functional cellular component and a substrate for several protein kinases. Here we investigated the interaction of protein kinase CKII (casein kinase II) and β-catenin. We show that CKII phosphorylates the N-terminal region of β-catenin and we...

...Lorenzo A. Pinna CK2 is an extremely conserved pleiotropic protein kinase with a growing list of more than 300 substrates, the majority of which are proteins implicated in signal transduction, gene expression and other nuclear functions. The CK2 phosphoacceptor sites are specified by multiple...

... to realise that Cdc2 and cyclins form a stoichiometric complex and that a cyclin subunit is necessary for the Cdc2 subunit to gain its protein kinase activity. Cyclins were first recognized as proteins whose abundance oscillates during the early cell cycles of marine invertebrate eggs and their connection...

... ). Translational attenuation mediated by an mRNA intron. Curr. Biol . 7 , 850 -859. Cox, J. S., Shamu, C. E. and Walter, P. ( 1993 ). Transcriptional induction of genes encoding endoplasmic reticulum resident proteins requires a transmembrane protein kinase. Cell 73 , 1197 -1206. Cox, J. S...

... established, there is now clear evidence for the requirement of additional mitotic protein kinases. Studies into the regulation of CDKs and other mitotic kinases have revealed that these enzymes undergo cell cycle dependent changes in subcellular distribution, suggesting that localization may contribute...

... + cyclin Xenopus eggs protein kinase J. Cell Sci. Suppl. 12, 99-116 (1989) Printed in Great Britain @ The Company of Biologists Limited 1989 99 Temporal regulation of cdc2 mitotic kinase activity and cyclin degradation in cell-free extracts of Xenopus eggs M A RIE-A N N E F E L I X 1, J O N A T H...

...GIULIO DRAETTA; DAVID BEACH Summary Recent experimental evidence has demonstrated the central role of the cdc2 protein kinase in the transition from G 2 to M phase in eukaryotic cells. We shall review our knowledge of the mechanisms which coordinate activation of the kinase with cell cycle-specific...