... ‡ Authors for correspondence ( mike.white@manchester.ac.uk ; julian.davis@manchester.ac.uk ) 6 6 2011 © 2011. 2011 Prolactin Transcription Pituitary Development Microscopy Funding This work was supported by the Wellcome Trust [grant numbers 067252, 082794]; the Society...

... prolactin or growth hormone. We found that DG regulates signaling by both hormones in a manner that is dependent on laminin-111 binding, but independent of the DG cytoplasmic domain, suggesting that it acts via a co-receptor mechanism reliant on DG-mediated laminin assembly. These results demonstrate...

... lactotrophic cells or intact pituitary tissue from bacterial artificial chromosome (BAC) transgenic rats in which a large prolactin genomic fragment directed expression of luciferase or destabilised enhanced green fluorescent protein (d2EGFP). Prolactin promoter activity in transgenic pituitaries varied...

.... Designed after the prolactin promoter-enhancer, it allows for the direct visualization of estrogen receptor α (ERα) and/or Pit-1 interactions at a physiologically regulated transcription locus. ERα- and ligand-dependent cofactor recruitment, large-scale chromatin modifications and transcriptional activity...

... transcription factor interactions in the living cell nucleus by fluorescence resonance energy transfer microscopy. Mol. Endocrinol. 12 , 1410 -1419. Day, R. N. and Maurer, R. A. ( 1989 ). The distal enhancer region of the rat prolactin gene contains elements conferring response to multiple hormones...

... rat pituitary lactotrophs. Secretory stimulation with high external [K + ] resulted in 100 exocytic sites per cell that were labeled by extracellular antibodies against the peptide hormone prolactin. Morphological analysis demonstrated that the prolactin was retained and internalized in intact dense...

... laminin co-operates with soluble factors such as prolactin to regulate tissue-specific gene expression. The mechanism of matrix and prolactin crosstalk and the activation of downstream signals are not fully understood. Because integrins organize the cytoskeleton, we analysed the contribution...