... interaction, and FLNA did not localize in RNA-rich stress granules (SGs). Disruption of the interaction was sufficient to promote phase-separated SG formation, and arsenite treatment further stimulated the formation of SGs. Taken together, these data identify G3BP1 as a new mechanobinding protein...

... by liquid–liquid phase separation. An intrinsically disordered region (IDR) at the N-terminus is essential for Dvl2 phase separation. Importantly, we identified the HECT-E3 ligase WWP2 as an essential driver of Dvl2 phase separation in vitro and in cells. We demonstrated that ubiquitylation of Dvl2 through...

...Wessel van Leeuwen; Dan T. M. Nguyen; Rianne Grond; Tineke Veenendaal; Catherine Rabouille; Ginny G. Farías ABSTRACT Phase separation of components of ER exit sites (ERES) into membraneless compartments, the Sec bodies, occurs in Drosophila cells upon exposure to specific cellular stressors, namely...

... Processing bodies Membraneless granules Phase separation Endoplasmic reticulum Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung http://dx.doi.org/10.13039/501100001711 310030B_163480 310030_185127 Universität Basel http://dx.doi.org/10.13039/100008375...

...Chujun Zhang; Wessel van Leeuwen; Marloes Blotenburg; Angelica Aguilera-Gomez; Sem Brussee; Rianne Grond; Harm H. Kampinga; Catherine Rabouille ABSTRACT The phase separation of the non-membrane bound Sec bodies occurs in Drosophila S2 cells by coalescence of components of the endoplasmic reticulum...

...Tetsuo Mioka; Tian Guo; Shiyao Wang; Takuma Tsuji; Takuma Kishimoto; Toyoshi Fujimoto; Kazuma Tanaka ABSTRACT Membrane phase separation to form micron-scale domains of lipids and proteins occurs in artificial membranes; however, a similar large-scale phase separation has not been reported...

... identified yeast Ataxin2 (Pbp1) as a negative regulator of TORC1 signaling ( Yang et al., 2019 ). Pbp1 was shown to bind TORC1, specifically during respiratory growth, and inhibits TORC1 signaling via a low-complexity region by a phase-separation mechanism ( Yang et al., 2019 ). Our genetic screen identified...

... guide Spitzenkörper vesicles to the hyphal tip. Actin cytoskeleton Fungal polarisome Macromolecular assembly Phase separation Summary: A review of how the molecular condensation machinery drives dynamic assembly of the yeast macromolecular polarisome complex, and thereby, its...

...Amy R. Strom; Clifford P. Brangwynne ABSTRACT Cells organize membrane-less internal compartments through a process called liquid–liquid phase separation (LLPS) to create chemically distinct compartments, referred to as condensates, which emerge from interactions among biological macromolecules...

... as how its deregulation is involved in multiple human diseases. p62 Sqstm1 Selective autophagy Phase separation Keap1–Nrf2 system Proteinopathies Sequestosome 1 (SQSTM1; also known as p62) is conserved in metazoans, and autophagy – the process with which SQSTM1 is primarily associated...

...) granules. RNP granules assemble through a concentration-dependent liquid–liquid phase separation of RNA-binding proteins that contain low-complexity sequence domains (LCDs). Interestingly, many factors that regulate microRNA (miRNA) biogenesis and alternative splicing are RNA-binding proteins that contain...

... , R. and Rosen , M. K. ( 2016 ). Compositional control of phase-separated cellular bodies . Cell   166 , 651 - 663 . 10.1016/j.cell.2016.06.010 Banani , S. F. , Lee , H. O. , Hyman , A. A. and Rosen , M. K. ( 2017 ). Biomolecular condensates: organizers...

...) in macromolecular phase separation. Potential contributions of HEAT–HEAT interactions, as well as cooperation between HEATs and IDRs, to mesoscale organelle assembly will be discussed. References Abe , S. , Nagasaka , K. , Hirayama , Y. , Kozuka-Hata , H. , Oyama , M. , Aoyagi , Y...