... complexes, both including a palmitoylated anchor, EFR3. Whereas both PI4KIIIα complexes support production of PI(4)P, the distinct functions of each complex and mechanisms underlying the interplay between them remain unknown. Here, we present roles for differential palmitoylation patterns within a tri...

... Akimzhanov , A. M. and Boehning , D. ( 2015 ). Rapid and transient palmitoylation of the tyrosine kinase Lck mediates Fas signaling . Proc. Natl. Acad. Sci. USA 112 , 11876 - 11880 . 10.1073/pnas.1509929112 Akimzhanov , A. M. , Wang , X. , Sun , J. and Boehning , D...

... of Biologists Ltd 2021 Summary: The acyltransferase DHHC21 is a novel component of the TCR signaling pathway that critically regulates activation and differentiation of CD4 + T cells by mediating rapid TCR-induced S-acylation of signaling proteins. Palmitoylation S-acylation T cell receptor...

...Jordan J. Shimell; Andrea Globa; Marja D. Sepers; Angela R. Wild; Nusrat Matin; Lynn A. Raymond; Shernaz X. Bamji ABSTRACT Palmitoylation is the most common post-translational lipid modification in the brain; however, the role of palmitoylation and palmitoylating enzymes in the nervous system...

... evidence by acyl-biotin exchange that β2 is S-acylated at Cys-182. Interestingly, we found that palmitoylation increases β2 association with detergent-resistant membranes. β2 localizes exclusively to the apical surface. However, depletion of plasma membrane cholesterol, or blocking intracellular...

... in the eukarya domain, with 23 distinct zDHHC-encoding genes in the human genome. zDHHC enzymes mediate the bulk of S-acylation (also known as palmitoylation) reactions in cells, transferring acyl chains to cysteine thiolates, and in so-doing affecting the stability, localisation and function of several thousand...

...Takeshi Harada; Ryota Sada; Yoshito Osugi; Shinji Matsumoto; Tomoki Matsuda; Mitsuko Hayashi-Nishino; Takeharu Nagai; Akihiro Harada; Akira Kikuchi ABSTRACT Cytoskeleton-associated protein 4 (CKAP4) is a palmitoylated type II transmembrane protein localized to the endoplasmic reticulum (ER). Here...

... ( Impagnatiello et al., 2001 ; Lim et al., 2002 ). However, it has been reported that the CRD is also modified by S-acylation ( Impagnatiello et al., 2001 ). S-acylation (also known as palmitoylation), is a process whereby fatty acids are attached onto cysteine residues. This modification is known to play...

... following nutrient deprivation. Our data shows that activated Gαi3 decreases Bcl-2 phosphorylation, whereas inhibitors of Gαi3, such as RGS4 and AGS3 (also known as GPSM1), markedly increase the levels of phosphorylated Bcl-2. Manipulation of the palmitoylation status and intracellular localization of RGS4...

...Bhavin S. Shah; Jordan J. Shimell; Shernaz X. Bamji ABSTRACT Protein palmitoylation is the most common post-translational lipid modification in the brain and is mediated by a family of 24 zDHHC enzymes. There has been growing interest in zDHHCs due to mounting evidence that these enzymes play key...

... referred to as palmitoylation. However, other fatty acids with different chain lengths and unsaturation can also be added to S-acylated proteins ( Muszbek et al., 1999 ). S-acylation not only targets peripheral proteins to membranes but also regulates protein trafficking, turnover and cell signalling...

... not essential for rapid unrestricted growth of ESCs under optimal conditions, the novel Gab1 variant (Gab1β) is required for LIF-mediated cell survival under conditions of limited nutrient availability. This enhanced survival is absolutely dependent upon a latent palmitoylation site that targets Gab1β directly...

... of internalized integral membrane proteins, such as the epidermal growth factor receptor (EGFR). We found that Rab7 is palmitoylated and that this modification is not required for membrane anchoring. Palmitoylated Rab7 colocalizes efficiently with and has a higher propensity to interact with retromer than...

... palmitoylation or other motifs contribute to the effective sorting of TRAP proteins. We found that palmitoylation is essential for some, but not all, TRAP proteins independent of their TMD length. We also provide evidence that palmitoylation and proximal sequences can modulate sorting of artificial proteins...

..., and the transcriptional activation of Elk-1 and the proliferation in response to IGF-1 were reduced in these cells. We found that IGF-1-independent flotillin-1 palmitoylation at Cys34 in the endoplasmic reticulum (ER) was required for the ER exit and the plasma membrane localization of flotillin-1 and IGF-1R. IGF-1...

... are poorly understood. Protein palmitoylation is a posttranslational lipid modification that plays an important role in protein trafficking and function. Here, we demonstrate that multiple desmosomal components are palmitoylated in vivo . Pharmacologic inhibition of palmitoylation disrupts desmosome assembly...

... in the transmembrane domain by Rho generates active sSpi, which is palmitoylated at its N-terminus. (B) Chimeric transmembrane proteins were created by fusing sSpi to Nrt (light green), Gli (dark green) or the transmembrane domain of CD2 (orange). All three chimeras have an HA tag (purple). The EGF domain...

... is a peripheral membrane protein, and palmitoylation of a cluster of four cysteine residues mediates its stable association with the membrane. Here, we report that palmitoylation also determines the precise intracellular distribution of SNAP25, and that mutating single palmitoylation sites enhances the amount...

... (BTC-ICD). We found that the proBTC cytoplasmic domain is palmitoylated and that palmitoylation is not required for ADAM10-dependent cleavage but is necessary for the stability and γ-secretase-dependent processing of BTC-CTF to generate BTC-ICD. Additionally, palmitoylation is required for nuclear...

... Intraflagellar transport Lipid rafts Palmitoylation Targeting Cilia and flagella are ancient organelles found in organisms spanning the eukaryotic lineage. The basic structure and biogenesis of cilia and flagella are highly conserved. With few exceptions, these organelles consist of a microtubule...