... is incompletely understood. Enhanced protein synthesis necessitates ribosome biogenesis at the nucleolus. Nucleolar size and activity are inextricably linked with altered gene expression. Here, we report changes in nucleolar morphology and function during myogenic differentiation. Immunofluorescence analysis...

.... Adjusted P -values are shown for each comparison. To maintain genome stability in the ribosomal DNA domain is particularly challenging given that it is located in the nucleolus. The nucleolus is a subnuclear compartment with a high density of nucleic acids and proteins that creates a distinct...

...Milad Jeilani; Karen Billington; Jack Daniel Sunter; Samuel Dean; Richard John Wheeler ABSTRACT The compartmentalised eukaryotic cell demands accurate targeting of proteins to the organelles in which they function, whether membrane-bound (like the nucleus) or non-membrane-bound (like the nucleolus...

..., p110β and PtdIns(3,4,5) P 3 were localised in the nucleolus, which correlated with high levels of 47S pre-rRNA. p110β inhibition led to a decrease in both 47S rRNA levels and cell proliferation. In conclusion, these results present a nucleolar role for p110β that may contribute to tumorigenesis in EC...

.... The nucleus is organized into various subcompartments, with the nucleolus being the most prominent. We have conducted a candidate-based genetic screen for nuclear shape abnormalities in S. cerevisiae to ask whether the nucleolus influences nuclear shape. We find that increasing nucleolar volume triggers a non...

... nuclear functions. Chromatin Nuclear organization Nucleolus Phase separation Compartmentalization is used throughout all kingdoms of biology to create functionally distinct units within a complex cellular environment. We usually think of these compartments as requiring a lipid bilayer...

... targeted nucleoli with carbon ions at the ion microbeam SNAKE. Localized ion irradiation with 1–100 carbon ions per point (about 0.3–30 Gy per nucleus) did not lead to overall reduced ribonucleotide incorporation in the targeted nucleolus or other nucleoli of the same cell. However, both 5-ethynyluridine...

.../ Summary: In humans, sirtuin 7 plays a key role in the synthesis of ribosomal subunits by controlling critical endonucleolytic cleavages of precursor ribosomal RNAs. Pre-rRNA processing Ribosome Sirtinol SIRT7 Nucleolus Deacetylase Nucleoli are membrane-lacking nuclear bodies...

... and structured illumination microscopy. We show that rDNA is replicated inside and outside the nucleolus, where active transcription occurs at the same time. Nascent rDNA shows a maximum of nucleolar associations during early S phase. In addition to EdU patterns typical for early or late S phase, we describe two...

...Sugako Ogushi; Kazuo Yamagata; Chikashi Obuse; Keiko Furuta; Teruhiko Wakayama; Martin M. Matzuk; Mitinori Saitou ABSTRACT The mammalian oocyte nucleolus, the most prominent subcellular organelle in the oocyte, is vital in early development, yet its key functions and constituents remain unclear. We...

... B23 depletion accelerated disassembly. Our results suggest that iPNB disassembly and nucleolus assembly might be coupled through RNA-dependent exchange of nucleolar proteins, creating a highly dynamic system with long-distance correlations between spatially distinct processes. References Ahn...

... nucleoli disappear, mitotic pre-ribosomal particles persist as observed in pre-nucleolar bodies (PNBs) during telophase. To further understand the relationship between the nucleolus and the PNBs, the presence and the fate of the mitotic pre-ribosomal particles during cell division were investigated. We...

...-like 3, GNL3, in invertebrate species). Several studies have recently pointed to an unexpected role of nucleostemin in safeguarding the genome integrity of stem and cancer cells. Since its discovery, the predominant presence of nucleostemin in the nucleolus has led to the notion that it might function...

... defined ( Li et al., 2012 ; Mao et al., 2009 ). Our data open up new avenues of research into the RelA ubiquitylation signature that targets the protein to the nucleolus, and the ubiquitin ligase complex associated with p300-acetylated COMMD1. Taken together, our data suggest the model outlined...

... the nucleolus was fine-tuned for a role in genome protection and cell-cycle control as the vertebrates evolved. We thank Hanhui Ma in the T.P. laboratory for advice on the 5-EU assay. Author contributions T.L., L.M., T.P. and R.Y.L.T. designed research; T.L., L.M., T.-C.L. and L.J.W performed...

... by sequestering Cdc14 phosphatase in the nucleolus before mitotic exit. In this study, we show that dnt1 + positively regulates G2/M transition during the cell cycle. By conducting epistasis analyses to measure cell length at septation in double mutant (for dnt1 and genes involved in G2/M control) cells, we found...

... 4,5-bisphosphate (PIP2) is a part of the protein complex on the active ribosomal promoter during transcription. PIP2 makes a complex with Pol I and the Pol I transcription factor UBF in the nucleolus. PIP2 depletion reduces Pol I transcription, which can be rescued by the addition of exogenous PIP2...

... for correspondence ( gary.stein@umassmed.edu ) 7 2 2012 © 2012. 2012 RUNX2 HDAC1 rRNA RNA polymerase I Osseous cell Nucleolus Histone acetylation Genetic and epigenetic mechanisms are coordinated by lineage-specific transcription factors to regulate the temporal expression of genes...

... streamlined efficient replication of the most heavily transcribed genomic loci while simultaneously reducing the risk of chromosome breaks and rDNA hyper-recombination. daniela.dimitrova@bbsrc.ac.uk 26 4 2011 © 2011. 2011 Gene expression Human rRNA genes Nucleolus Replication...

.... Since data on the intranucleolar localization of p53 are controversial, we have analyzed in detail its subnucleolar distribution. Our results show that p53 does not localize to one of the well-known structural components of the nucleolus involved in ribosome biogenesis, but rather occupies distinct...