... demonstrate that U-ExM allows imaging of the microtubule cytoskeleton and its associated spindle pole body, notably unveiling the Sfi1p–Cdc31p spatial organization on the appendage bridge structure. In S. pombe , we validate the method by monitoring the homeostatic regulation of nuclear pore complex number...

.... Soc. 131 , 5018 - 5019 . 10.1021/ja809117z Daigle , N. , Beaudouin , J. , Hartnell , L. , Imreh , G. , Hallberg , E. , Lippincott-Schwartz , J. and Ellenberg , J. ( 2001 ). Nuclear pore complexes form immobile networks and have a very low turnover in live...

...Joanna Kalita; Larisa E. Kapinos; Roderick Y. H. Lim ABSTRACT Macromolecular cargoes are asymmetrically partitioned in the nucleus or cytoplasm by nucleocytoplasmic transport (NCT). At the center of this activity lies the nuclear pore complex (NPC), through which soluble factors circulate...

...Mohamed Hamed; Birgit Caspar; Sarah A. Port; Ralph H. Kehlenbach ABSTRACT Nup214 is a major nucleoporin on the cytoplasmic side of the nuclear pore complex with roles in late steps of nuclear protein and mRNA export. It interacts with the nuclear export receptor CRM1 (also known as XPO1) via...

...Giulia Paci; Joana Caria; Edward A. Lemke ABSTRACT Bidirectional transport of macromolecules across the nuclear envelope is a hallmark of eukaryotic cells, in which the genetic material is compartmentalized inside the nucleus. The nuclear pore complex (NPC) is the major gateway to the nucleus...

...Matías Capella; Lucía Martín Caballero; Boris Pfander; Sigurd Braun; Stefan Jentsch ABSTRACT Misassembled nuclear pore complexes (NPCs) are removed by sealing off the surrounding nuclear envelope (NE), which is conducted by the endosomal sorting complexes required for transport (ESCRT) machinery...

...Tadashi Makio; Richard W. Wozniak ABSTRACT Nuclear pore complexes (NPCs) control gene expression by regulating the bi-directional exchange of proteins and RNAs between nuclear and cytoplasmic compartments, including access of transcriptional regulators to the nucleoplasm. Here, we show...

...Suncica Barbato; Larisa E. Kapinos; Chantal Rencurel; Roderick Y. H. Lim ABSTRACT Ran is a small GTPase whose nucleotide-bound forms cycle through nuclear pore complexes (NPCs) to direct nucleocytoplasmic transport (NCT). Generally, Ran guanosine triphosphate (RanGTP) binds cargo-carrying...

...Hanne Folz; Carlos A. Niño; Surayya Taranum; Stefanie Caesar; Lorenz Latta; François Waharte; Jean Salamero; Gabriel Schlenstedt; Catherine Dargemont ABSTRACT The nuclear pore complex (NPC) is the major conduit for nucleocytoplasmic transport and serves as a platform for gene regulation and DNA...

...Melpomeni Platani; Itaru Samejima; Kumiko Samejima; Masato T. Kanemaki; William C. Earnshaw ABSTRACT In metazoa, the Nup107 complex (also known as the nucleoporin Y-complex) plays a major role in formation of the nuclear pore complex in interphase and is localised to kinetochores in mitosis...

... Nuclear pore complex 53BP1 BRCA1 DNA damage When genomic DNA is damaged, a protective response rapidly ensues involving events ranging from local changes in chromatin and intricate mechanisms of DNA repair to concomitant cell cycle arrest ( Daley and Sung, 2014 ; Smith-Roe et al., 2015...

...Vincent Duheron; Nadine Nilles; Sylvia Pecenko; Valérie Martinelli; Birthe Fahrenkrog ABSTRACT The nuclear basket of nuclear pore complexes (NPCs) is composed of three nucleoporins: Nup153, Nup50 and Tpr. Nup153 has a role in DNA double-strand break (DSB) repair by promoting nuclear import of 53BP1...

...Jiong Ma; Joseph M. Kelich; Samuel L. Junod; Weidong Yang ABSTRACT The nuclear pore complex (NPC), composed of ∼30 different nucleoporins (Nups), is one of the largest supramolecular structures in eukaryotic cells. Its octagonal ring scaffold perforates the nuclear envelope and features a unique...

...Marion Weberruss; Wolfram Antonin ABSTRACT The nucleus is enclosed by the nuclear envelope, a double membrane which creates a selective barrier between the cytoplasm and the nuclear interior. Its barrier and transport characteristics are determined by nuclear pore complexes (NPCs) that are embedded...

... -axis, to detect the three dimensional (3D) positions of nuclear pore complexes (NPCs) in the nuclear envelope. Here, we used a method called NucQuant to accurately estimate the geometry of nuclei in 3D throughout the cell cycle. To increase the robustness of the statistics, we aggregated thousands...

... of embryonic large molecule derived from yolk sac (ELYS; also known as AHCTF1), a component of the nuclear pore complex, from cells perturbs the localization of LBR to the INM, but little is known about the underlying molecular mechanism. In this study, we found that the depletion of ELYS promoted LBR...

...; however, only two integral protein complexes span the nuclear envelope and connect the inside of the nucleus to the cytoplasm. The nuclear pore complex (NPC) acts as a gateway for molecular exchange between the interior of the nucleus and the cytoplasm, whereas so-called LINC complexes physically link...

... tubular ER formation, whereas overexpression of TMEM170A induces ER sheet formation, indicating that TMEM170A is a newly discovered ER-sheet-promoting protein. Additionally, downregulation of TMEM170A alters nuclear shape and size, decreases the density of nuclear pore complexes (NPCs) in the nuclear...

... nuclear expansion and import of large cargo molecules in Xenopus laevis egg and embryo extracts, consistent with our observation that NTF2 reduces the diameter of the nuclear pore complex (NPC) in a Ran-binding-dependent manner. Furthermore, we demonstrate that ectopic NTF2 expression in Xenopus embryos...

... of chromatin and formation of a functional nuclear envelope. Here, we report that the histone demethylase LSD1 (also known as KDM1A) plays a crucial role in nuclear assembly at the end of mitosis. Downregulation of LSD1 in cells extends telophase and impairs nuclear pore complex assembly. In vitro , LSD1...