...Hanna Thoma; Luisa Grünewald; Silke Braune; Elisabeth Pasch; Manfred Alsheimer ABSTRACT SUN domain proteins are conserved proteins of the nuclear envelope and key components of the LINC complexes (for ‘linkers of the nucleoskeleton and the cytoskeleton’). Previous studies have demonstrated...

...Sherman Foo; Amaury Cazenave-Gassiot; Markus R. Wenk; Snezhana Oliferenko ABSTRACT Nuclear envelope (NE) expansion must be controlled to maintain nuclear shape and function. The nuclear membrane expands massively during closed mitosis, enabling chromosome segregation within an intact NE...

... of nuclear stiffness. Cancer Metastasis Cell migration Cytoskeleton Nucleus Nuclear envelope Emerin Src Mechanotransduction Melanoma Research Alliance http://dx.doi.org/10.13039/100005190 688232 American Cancer Society http://dx.doi.org/10.13039/100000048 RSG-20-019...

...Anthony Fernandez; Markville Bautista; Liying Wu; Fabien Pinaud ABSTRACT Emerin is an integral nuclear envelope protein that participates in the maintenance of nuclear shape. When mutated or absent, emerin causes X-linked Emery-Dreifuss muscular dystrophy (EDMD). To understand how emerin takes part...

...Zeinab Jahed; Nya Domkam; Jessica Ornowski; Ghafar Yerima; Mohammad R. K. Mofrad ABSTRACT Large protein complexes assemble at the nuclear envelope to transmit mechanical signals between the cytoskeleton and nucleoskeleton. These protein complexes are known as the linkers of the nucleoskeleton...

... in interphase and becomes dumbbell shaped during mitotic division to facilitate the transfer of one nucleus to the daughter cell. Because yeast cells undergo closed mitosis, the nuclear envelope remains intact throughout the cell cycle. The pathways that regulate nuclear shape are not well characterized...

... DNA, histones and various other nuclear proteins, including those enriched at the nuclear envelope, BAF appears to be essential for replicating cells to protect the genome and enable cell division. Cellular processes, such as innate immunity, post-mitotic nuclear reformation, repair of interphase...

... in glycosylphosphatidylinositol (GPI), localizes to the nuclear envelope (NE). Although this NE localization is essential for Drosophila development, the underlying molecular mechanism remains unknown. To elucidate this mechanism, we identified PIG-B-interacting proteins by performing immunoprecipitation followed by proteomic...

..., the nuclear envelope possesses unique mechanical properties, which enables it to resist cytoskeletal forces. Here, we review studies that are beginning to provide quantitative insights into nuclear membrane mechanics. We discuss how the mechanical properties of the fused nuclear membranes mediate...

...Menno Spits; Lennert J. Janssen; Lenard M. Voortman; Raymond Kooij; Anna C. M. Neefjes; Huib Ovaa; Jacques Neefjes ABSTRACT Upon nuclear envelope (NE) fragmentation in the prometaphase, the nuclear and cytosolic proteomes mix and must be redefined to reinstate homeostasis. Here, by using...

.... Elo2 Fatty acid elongation Fission yeast Lem2 Nuclear envelope Fatty acids are components of lipids, which constitute the intracellular membranes of eukaryotic cells, and are classified by their carbon chain length. Long-chain fatty acids have a chain length of 11-20 carbon atoms (C11-C20...

...Hardeep K. Gumber; Joseph F. McKenna; Amado L. Estrada; Andrea F. Tolmie; Katja Graumann; Hank W. Bass ABSTRACT The linker of nucleoskeleton to cytoskeleton (LINC) complex is an essential multi-protein structure spanning the nuclear envelope. It connects the cytoplasm to the nucleoplasm, functions...

..., is synthesized by the PIG enzymes. Most PIGs are localized to the endoplasmic reticulum (ER), but Drosophila PIG-B (DmPIG-B) is localized to the nuclear envelope (NE). To determine whether the NE localization of DmPIG-B is functionally important, we defined the determinants of localization and generated an ER...

....-A. , Liou , G.-G. , Yang , C.-S. and Chi , Y.-H. ( 2014 ). Dysregulated interactions between lamin A and SUN1 induce abnormalities in the nuclear envelope and endoplasmic reticulum in progeric laminopathies . J. Cell Sci.   127 , 1792 - 1804 . 10.1242/jcs.139683 Cheutin , T...

... eukaryotic chromosome segregation in the light of biological diversity, covering a number of experimental approaches used in comparative biology studies. Chromosome segregation Comparative biology Evolution Mitosis Nuclear envelope Spindle While ∼70% of the extant kinetochore proteins have...

... Enarson , P. , Enarson , M. , Bastos , R. and Burke , B. ( 1998 ). Amino-terminal sequences that direct nucleoporin nup153 to the inner surface of the nuclear envelope . Chromosoma   107 , 228 - 236 . 10.1007/s004120050301 Fornerod , M. , van Deursen , J. , van Baal...

...Marta G. Lete; Richard D. Byrne; Alicia Alonso; Dominic Poccia; Banafshé Larijani ABSTRACT Regulation of nuclear envelope dynamics is an important example of the universal phenomena of membrane fusion. The signalling molecules involved in nuclear membrane fusion might also be conserved during...

.... SIRT2 has also been implicated in the pathology of cancer, neurodegenerative diseases and progeria. Here, we show that SIRT2 depletion or overexpression causes nuclear envelope reassembly defects. We link this phenotype to the recently identified regulator of nuclear envelope reassembly ANKLE2. ANKLE2...

...Marion Weberruss; Wolfram Antonin ABSTRACT The nucleus is enclosed by the nuclear envelope, a double membrane which creates a selective barrier between the cytoplasm and the nuclear interior. Its barrier and transport characteristics are determined by nuclear pore complexes (NPCs) that are embedded...

...Yasuhiro Mimura; Masatoshi Takagi; Michaela Clever; Naoko Imamoto ABSTRACT Lamin B receptor (LBR), an inner nuclear membrane (INM) protein, contributes to the functional integrity of the nucleus by tethering heterochromatin to the nuclear envelope. We have previously reported that the depletion...