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Keywords: Nanobody
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Journal Articles
J Cell Sci (2022) 135 (14): jcs259168.
Published: 18 July 2022
... over the traditional antibody. Here, we focus on nanobodies and the non-antibody binding scaffold proteins called Affimers. We explain how they are generated, selected and produced, and we describe how their small size, high binding affinity and specificity provides them with many advantages compared...
Includes: Supplementary data
Journal Articles
J Cell Sci (2019) 132 (21): jcs234955.
Published: 6 November 2019
... additional tags, functionalized nanobodies that bind GFP can be used to extend the functionality of the GFP tag. We developed functionalized nanobodies, which we termed ‘dongles’, that could add, for example, an FKBP tag to a GFP-tagged protein of interest, enabling knocksideways experiments in GFP knock...
Includes: Supplementary data
Journal Articles
J Cell Sci (2017) 130 (7): 1299–1306.
Published: 1 April 2017
... super-resolution single-point edge-excitation subdiffraction (SPEED) microscopy and nanobody-specific labeling to reveal the spatial distribution of scaffold Nups within three separate layers in the native NPC with a precision of ∼3 nm. Our data reveal both the radial and axial spatial distributions...
Includes: Supplementary data
Journal Articles
J Cell Sci (2013) 126 (21): 4900–4912.
Published: 1 November 2013
... of receptor clustering in EGFR internalization we aimed to induce receptor clustering by using biparatopic nanobodies that bind intermolecularly to EGFRs without stimulating kinase activity. Different anti-EGFR VHH constructs were used (Mono1–Mono4) as building blocks for the biparatopic nanobodies ( Fig. 1A...
Includes: Supplementary data