... over the traditional antibody. Here, we focus on nanobodies and the non-antibody binding scaffold proteins called Affimers. We explain how they are generated, selected and produced, and we describe how their small size, high binding affinity and specificity provides them with many advantages compared...

... additional tags, functionalized nanobodies that bind GFP can be used to extend the functionality of the GFP tag. We developed functionalized nanobodies, which we termed ‘dongles’, that could add, for example, an FKBP tag to a GFP-tagged protein of interest, enabling knocksideways experiments in GFP knock...

... super-resolution single-point edge-excitation subdiffraction (SPEED) microscopy and nanobody-specific labeling to reveal the spatial distribution of scaffold Nups within three separate layers in the native NPC with a precision of ∼3 nm. Our data reveal both the radial and axial spatial distributions...

... endocytosis Transmembrane domain VHH Nanobody The receptor for the epidermal growth factor (EGFR, HER1 or ErbB1) is a receptor tyrosine kinase that, together with its family members HER2, HER3 and HER4, belongs to the ErbB family of growth factor receptors ( Yarden, 2001 ). This family of receptor...