... and defective formation of growth cones in young neurons. Here we present new evidence that upon Rac1 and Rac3 ablation, centrosome, Golgi complex and lysosome positioning are significantly perturbed, thus affecting both interneuron migration and axon growth. Moreover, for the first time we provide evidence...

... focal adhesion disassembly. LL5β is phosphorylated by MINK1, promoting its interaction with CLASP proteins. Using a kinase inhibitor, we demonstrate that the enzymatic activity of MINK1 is involved in PRICKLE1–LL5β complex assembly and localization, as well as in cell migration. Analysis of gene...

... to be recycled back to the cell membrane. We show that PDGFRβ homodimer activation results in a greater amplitude of phospho-ERK1/2 and phospho-AKT signaling, as well as increased proliferation and migration. Finally, we demonstrate that inhibition of clathrin-mediated endocytosis leads to changes in cellular...

... characterized, particularly during development . Although exosomes released by invasive metastatic cells alter migration of neighboring cells in culture, it is unclear whether cancer cells misappropriate exosomes released by healthy differentiated cells or reactivate dormant developmental programs that include...

...Kristiane Søreng; Serhiy Pankiv; Camilla Bergsmark; Ellen M. Haugsten; Anette K. Dahl; Laura R. de la Ballina; Ai Yamamoto; Alf H. Lystad; Anne Simonsen ABSTRACT Cell migration is a complex process underlying physiological and pathological processes such as brain development and cancer metastasis...

... was maintained despite cells readily delaminating from epithelial sheets. Distinct from the prominent filopodia in p110α H1047R -expressing cells, p110β induced formation of lamellipodia, and these cells migrated with significantly greater velocity and decreased directionality. p110β-induced phenotypic...

... , A. and Kuo , J.-C . ( 2016 ). Focal adhesion kinase-dependent focal adhesion recruitment of SH2 domains directs SRC into focal adhesions to regulate cell adhesion and migration . Sci. Rep. 5 , 18476 . 10.1038/srep18476 Xue , Y. , Liu , Z. , Cao , J. and Re , J . ( 2011...

...Nathan H. Roy; Sarah Hyun Ji Kim; Alexander Buffone, Jr; Daniel Blumenthal; Bonnie Huang; Sangya Agarwal; Pamela L. Schwartzberg; Daniel A. Hammer; Janis K. Burkhardt ABSTRACT T cell entry into inflamed tissue requires firm adhesion, cell spreading, and migration along and through the endothelial...

... and the transmembrane heparan sulfate proteoglycan syndecan-4 in influencing adhesion, migration and angiogenesis. Knockdown of ADAMTS-1 in endothelial cells resulted in a parallel reduction in cell surface syndecan-4, attributable to increased matrix metalloproteinase-9 (MMP9) activity. Knockdown of either ADAMTS-1...

...Daniel A. Girón-Pérez; Eduardo Vadillo; Michael Schnoor; Leopoldo Santos-Argumedo; Ana-Maria Lennon-Duménil ABSTRACT The inclusion of lymphocytes in high endothelial venules and their migration to the lymph nodes are critical steps in the immune response. Cell migration is regulated by the actin...

...-polarized hMDMs. Using PTPε-specific siRNAs and expression of RPTPε and cyt-PTPε, we found that RPTPε is involved in monocyte adhesion and migration of M2-polarized hMDMs in response to LTD 4 . Altered organization of podosomes and higher phosphorylation of the inhibitory Y-722 residue of ROCK2 was also...

...Kim Kenwrick; Amrita Mukherjee; Andrew D. Renault ABSTRACT During development, many cell types migrate along stereotyped routes determined through deployment of cell surface or secreted guidance molecules. Although we know the identity of many of these molecules, the distances over which...

... and resulted in a thicker epidermal layer after reepithelialization. Conversely, preventing Cx43 phosphorylation by mitogen-activated protein kinases (MAPKs) through mutation significantly increased the rate of wound closure in vivo . Defects in migration, but not proliferation, in all mutants were partially...

... in Ahi1 −/− MEFs restored ciliary length, increased ciliary recruitment of Arl13b and augmented Arl13b stability. Finally, Ahi1 −/− MEFs displayed defects in cell motility and Pdgfr-α-dependent migration. Overall, our findings support molecular mechanisms underlying JBTS etiology that involve: (1...

... into integrin β4 dynamics in response to a migratory stimulus (scratch wound) by live-cell video microscopy. An increase in integrin β4 expression in cells proximal to the wound edge was evident, and a population of integrin β4-expressing cells that exhibited unusually rapid migration was identified...

...Bertille Bance; Shailaja Seetharaman; Cécile Leduc; Batiste Boëda; Sandrine Etienne-Manneville ABSTRACT Microtubules play a crucial role in mesenchymal migration by controlling cell polarity and the turnover of cell adhesive structures on the extracellular matrix. The polarized functions...

... , in cultured Schwann cells and find that tau is present in these cells. Gene silencing of Mapt promoted Schwann cell proliferation and inhibited Schwann cell migration and differentiation. In vivo application of Mapt siRNA suppressed the migration of Schwann cells after sciatic nerve injury. Consistent...

... of podoplanin expression on the migratory potential of MSCs and their interactions with circulating platelets. Expression of podoplanin significantly enhanced the migration of MSCs compared to MSCs lacking podoplanin. Rac-1 inhibition altered the membrane localisation of podoplanin and in turn significantly...

... rearrangements drive macrophage recruitment to wounds in Drosophila . We find that the actin-regulatory protein Ena is a master regulator of lamellipodial dynamics in migrating macrophages, where it remodels the cytoskeleton to form linear filaments that can then be bundled together by the cross-linker Fascin...

...Charlotte M. de Winde; Alexandra L. Matthews; Sjoerd van Deventer; Alie van der Schaaf; Neil D. Tomlinson; Erik Jansen; Johannes A. Eble; Bernhard Nieswandt; Helen M. McGettrick; Carl G. Figdor; Michael G. Tomlinson; Sophie E. Acton; Annemiek B. van Spriel ABSTRACT Cell migration is central...