...Nami Haruta; Eisuke Sumiyoshi; Yu Honda; Masahiro Terasawa; Chihiro Uchiyama; Mika Toya; Yukihiko Kubota; Asako Sugimoto ABSTRACT The γ-tubulin complex (γTuC) is a widely conserved microtubule nucleator, but some of its components, namely GCP4, GCP5 and GCP6 (also known as TUBGCP4, TUBGCP5...

...Kristen J. Verhey; Ryoma Ohi ABSTRACT Microtubules are critical for a variety of important functions in eukaryotic cells. During intracellular trafficking, molecular motor proteins of the kinesin superfamily drive the transport of cellular cargoes by stepping processively along the microtubule...

...-regulating sequences, correlate with colorectal cancer. The cellular roles of APC in mitosis are widely studied, but the molecular mechanisms of its interaction with the cytoskeleton are poorly understood. Here, we investigated how APC-C regulates microtubule properties, and found that it promotes both...

...Olivera Mitevska; Pak Wing Lam; Lydia Daly; Philip Auckland ABSTRACT Mitotic cell division requires that kinetochores form microtubule attachments that can segregate chromosomes and control mitotic progression via the spindle assembly checkpoint. During prometaphase, kinetochores shed a domain...

...Ali Nick Maleki; Pim J. Huis in 't Veld; Anna Akhmanova; Marileen Dogterom; Vladimir A. Volkov ABSTRACT Microtubules are dynamic cytoskeletal filaments that can generate forces when polymerizing and depolymerizing. Proteins that follow growing or shortening microtubule ends and couple forces...

... unknown. Here, we investigate the role of actin, Rho GTPases and microtubules, which are major players in CIE processes, in this mechanism. We show that the actin cytoskeleton is dynamically associated with endoA3- and CD166-positive endocytic carriers, and that its perturbation strongly inhibits...

...Toni McHugh; Julie P. I. Welburn ABSTRACT The precise regulation of microtubule length during mitosis is essential to assemble and position the mitotic spindle and segregate chromosomes. The kinesin-13 Kif2C or MCAK acts as a potent microtubule depolymerase that diffuses short distances...

...Emmanuel T. Nsamba; Mohan L. Gupta, Jr ABSTRACT The microtubule cytoskeleton is assembled from the α- and β-tubulin subunits of the canonical tubulin heterodimer, which polymerizes into microtubules, and a small number of other family members, such as γ-tubulin, with specialized functions. Overall...

...Joseph Atherton; Melissa Stouffer; Fiona Francis; Carolyn A. Moores ABSTRACT Neurons extend axons to form the complex circuitry of the mature brain. This depends on the coordinated response and continuous remodelling of the microtubule and F-actin networks in the axonal growth cone. Growth cone...

... ABSTRACT Septins, a family of GTP-binding proteins that assemble into higher order structures, interface with the membrane, actin filaments and microtubules, and are thus important regulators of cytoarchitecture. Septin 9 (SEPT9), which is frequently overexpressed in tumors and mutated in hereditary...

...Akanksha Thawani; Sabine Petry ABSTRACT As one of four filament types, microtubules are a core component of the cytoskeleton and are essential for cell function. Yet how microtubules are nucleated from their building blocks, the αβ-tubulin heterodimer, has remained a fundamental open question since...

...Janet K. Jang; Amy C. Gladstein; Arunika Das; Joanatta G. Shapiro; Zachary L. Sisco; Kim S. McKim ABSTRACT Meiosis in female oocytes lacks centrosomes, the microtubule-organizing centers. In Drosophila oocytes, meiotic spindle assembly depends on the chromosomal passenger complex (CPC...

...T. Lynne Blasius; Yang Yue; RaghuRam Prasad; Xinglei Liu; Arne Gennerich; Kristen J. Verhey ABSTRACT The kinesin-4 member KIF7 plays critical roles in Hedgehog signaling in vertebrate cells. KIF7 is an atypical kinesin as it binds to microtubules but is immotile. We demonstrate that, like...

...Chengye Feng; Joseph M. Cleary; Gregory O. Kothe; Michelle C. Stone; Alexis T. Weiner; James I. Hertzler; William O. Hancock; Melissa M. Rolls ABSTRACT Axons and dendrites are distinguished by microtubule polarity. In Drosophila , dendrites are dominated by minus-end-out microtubules, whereas axons...

...Neha Khetan; Gérard Pruliere; Celine Hebras; Janet Chenevert; Chaitanya A. Athale ABSTRACT Radial microtubule (MT) arrays or asters determine cell geometry in animal cells. Multiple asters interacting with motors, such as those in syncytia, form intracellular patterns, but the mechanical principles...

...Patricia Wadsworth ABSTRACT During anaphase, a microtubule-containing structure called the midzone forms between the segregating chromosomes. The midzone is composed of an antiparallel array of microtubules and numerous microtubule-associated proteins that contribute to midzone formation...

... play an important role in cellular function and morphology. The biophysical properties of cells and tissues are determined by the cytoskeleton, consisting of dynamic networks of F-actin and microtubules, molecular motors, crosslinkers and other associated proteins, among other factors such as cell–cell...

.... Using Chlamydomonas , we attenuated IFT-based tubulin transport of GFP–β-tubulin by altering the IFT74N–IFT81N tubulin-binding module and the C-terminal E-hook of tubulin. E-hook-deficient GFP–β-tubulin was incorporated into the axonemal microtubules, but its transport frequency by IFT was reduced...

...Christopher Nosala; Kari D. Hagen; Nicholas Hilton; Tiffany M. Chase; Kelci Jones; Rita Loudermilk; Kristofer Nguyen; Scott C. Dawson ABSTRACT Giardia lamblia , a widespread parasitic protozoan, attaches to the host gastrointestinal epithelium by using the ventral disc, a complex microtubule (MT...

.... and Kirschmeier , P. ( 2000 ). Farnesyl transferase inhibitors block the farnesylation of CENP-E and CENP-F and alter the association of CENP-E with the microtubules . J. Biol. Chem.   275 , 30451 - 30457 . 10.1074/jbc.M003469200 Brown , K. D. , Wood , K. W. and Cleveland , D. W...