... microfilament regulation. Studies on Drosophila MICAL indicate that its depletion affects actin architecture and bristle morphology ( Hung et al., 2010 ). Accordingly, we next assessed the effect of MICAL1 and MICAL2 depletion on cellular F-actin. As demonstrated, RNA interference (RNAi) was successful...

.... and Beisson, J. ( 1988 ). The cytoskeleton. In Paramecium (ed. H.-D. Görtz), pp. 363 -392. Berlin, Heidelberg, New York: Springer-Verlag. Cohen, J., Garreau De Loubresse, N. and Beisson, J. ( 1984 ). Actin microfilaments in Paramecium : localization and role in intracellular movements. Cell Motil...

... to this work ‡ Present address: MGH Cancer Center and Harvard Medical School, Chalestown, MA, USA 15 8 2005 © The Company of Biologists Limited 2005 2005 Microfilaments Keratinocytes Actin bundles Cadherins Cell-cell contact Epithelial cells are morphologically polarized...

... Limited 2005 2005 Cell-matrix adhesion Cell motility Microfilaments Phosphatidylinositol-4,5-bisphosphate Vinculin Cell migration requires a regulated interplay of actin-filament dynamics and turnover of cell-matrix adhesions ( Pollard and Borisy, 2003 ; Ridley et al., 2003 ). Both...

... and induces process formation by discoid IAR2 epitheliocytes ( Fig. 9b , Table 1 ). In both types of cell, microfilament bundles disappeared; the circular actin bundles of epitheliocytes are not present after incubation with Y27632 ( Fig. 9b,d ). In fibroblasts, Y27632 did not significantly affect average...

... been shown regarding this. * Author for correspondence (e-mail: rkothary@ohri.ca ) 10 7 2003 © The Company of Biologists Limited 2003 2003 Plakin Cytoskeleton Microfilaments Nucleus Focal adhesions Bullous pemphigoid antigen 1 (Bpag1) was originally identified...

.... Part II, which focuses on how these structural networks influence information processing networks, appears in the next issue. (e-mail: donald.ingber@tch.harvard.edu ) © The Company of Biologists Limited 2003 2003 Cytoskeleton Microfilaments Microtubules Intermediate filaments...

.... It is well known that spatial patterns of microfilament bundles and of the microtubular system are quite different in epitheliocytes and in fibroblasts. These differences extend to microtubular dynamics in cytoplasts prepared from the cells of these two types ( Rodionov et al., 1999 ). Probably, differences...

... mitosis. Other microfilament- and microtubule-dependent processes appear normal in cyk-1 mutant embryos, indicating that cyk-1 regulates a specific subset of cytoskeletal functions. Because cytokinesis initiates normally and cleavage furrows ingress extensively in cyk-1 mutant embryos, we propose...

... of vitronectin receptor, the major integrin of osteoclasts, and cytoskeletal proteins that it may be linked to. Double staining for F-actin and vinculin, and for vinculin with talin, revealed that cytoskeletal organization differs at various activation states of osteoclasts. Microfilament structures were flat...

... domain is represented by microfilaments localized in the plasma membrane within the microprojections and membrane infoldings. At a slightly lower focal level, rhodamine-phalloidin labelling is concentrated in small irregular aggregates, which are localized around the dividing nuclei. Our results indicate...

... determination independent of changes in transmembrane osmotic pressure, membranes of adherent cells were permeabilized with saponin (25 g/ml) using a buffer that maintains the functional integrity of contractile microfilaments. Realtime videomicroscopic studies revealed that addition of 250 M ATP resulted...

...Sergio Schenkman; Renato A. Mortara ABSTRACT We show here that HeLa cell microfilaments can be stained by phalloidin at the sites of invasion of Trypanosoma cruzi trypomastigotes, one of the infective stages of this protozoan parasite. Concurrently, a projection of the HeLa cell plasmalemma...

... untreated cells or those treated with inactive phorbol esters did not form these structures. * Author for correspondence 09 08 1991 28 10 1991 © 1992 by Company of Biologists 1992 protein kinase C fibronectin microfilaments integrins focal adhesions Adhesion...

... conspicuously different from those of the basement membrane of the follicular epithelium. In the latter structure laminin was stained in a pattern of parallel stripes oriented perpendicular to the long axis of the follicle; microfilament bundles at the adjacent basal side of the follicle cells have the same...

...Christel R. Schopfer; Peter K. Hepler ABSTRACT The cellular pattern and distribution of membranes have been analyzed during cytokinesis in pollen mother cells of Tradescantia and compared with those of actin microfilaments (MFs) and microtubules (MTs). Membranes have been stained with DiOC 6 (3...

... larynx (HEp-2) epithelial cells to characterize bacterial adherence, invasion and penetration through epithelial monolayers. Epithelial cell microfilaments were required for bacterial internalization and surrounded the bacteria as they were internalized. The bacteria entered membrane-bound vacuoles...

... 16 12 1987 © 1988 by Company of Biologists 1988 microtubules microfilaments tip growth anti-cytoskeletal drugs moss. Restriction of cell expansion to a local specialized zone of the cell cortex is the characteristic mode of growth displayed by a wide range of filamentous...

... and actin-containing microfilaments, particularly at substratum adhesion points, are involved in various aspects of reticulopodial motility, particularly network morphogenesis and cell body locomotion. • Author for correspondence 09 09 1987 12 11 1987 © 1988 by Company of Biologists...

... that the Ca 2+ control of the functional state of 70 K protein might be one of the prime factors in the triggering of rapid assembly and disassembly of microfilaments within macrophages. * Author for correspondence 12 02 1987 27 04 1987 © 1987 by Company of Biologists 1987...