...Jeffrey A. Hadwiger; Huaqing Cai; Ramee G. Aranda; Saher Fatima ABSTRACT The Dictyostelium atypical mitogen-activated protein kinase (MAPK) Erk2 is required for chemotactic responses to cAMP as amoeba undergo multicellular development. In this study, Erk2 was found to be essential for the cAMP...

...Neal R. Rasmussen; David J. Reiner ABSTRACT The extracellular signal-regulated kinases (ERKs) are mitogen-activated protein kinases (MAPKs) that are utilized downstream of Ras to Raf to MEK signaling to control activation of a wide array of targets. Activation of ERKs is elevated in Ras-driven...

...Aditi Prabhakar; Beatriz González; Heather Dionne; Sukanya Basu; Paul J. Cullen ABSTRACT Mitogen-activated protein kinase (MAPK) pathways control cell differentiation and the response to stress. In Saccharomyces cerevisiae , the MAPK pathway that controls filamentous growth (fMAPK) shares...

... ( Brown et al., 1997 ; Chen et al., 1997 )]. The GIC2 gene was a target of the fMAPK pathway ( Fig. S1E ; see also MacIsaac et al., 2006 ). Msb2p, Sho1p and Ste11p regulate two MAPK pathways [fMAPK and high-osmolarity glycerol response (HOG) ( Saito, 2010 )]. A pathway-specific regulator...

... on chemoattractant gradient detection in yeast, which occurs at a motile polarity patch. It further describes the stabilization and hyperpolarization process underlying cell fusion. Cell fusion Cell polarity Chemotropism Pheromone gradient Cdc42 MAPK S. cerevisiae S. pombe All cells...

... anisotropy, focal adhesion density and cell stiffness were characteristics associated with efficient passage through constraints. By comparing transcriptomic profiles between the parental and selected populations, increased Ras/MAPK signalling was linked with cytoskeleton rearrangements and cell softening...

...Antonio Serrano; Julia Illgen; Ulrike Brandt; Nils Thieme; Anja Letz; Alexander Lichius; Nick D. Read; André Fleißner ABSTRACT Mitogen-activated protein kinases (MAPKs) are conserved regulators of proliferation, differentiation and adaptation in eukaryotic cells. Their activity often involves...

... relocalized in the cytosol, Net1A stimulates cell motility and extracellular matrix invasion. In the present work, we investigated mechanisms responsible for the cytosolic relocalization of Net1A. We demonstrate that inhibition of MAPK pathways blocks Net1A relocalization, with cells being most sensitive...

...Eric T. Hall; Esther M. Verheyen ABSTRACT Wnt/Wingless (Wg) and Ras–MAPK signaling both play fundamental roles in growth and cell fate determination, and when dysregulated, can lead to tumorigenesis. Several conflicting modes of interaction between Ras–MAPK and Wnt signaling have been identified...

... in mitogen-activated protein kinase (MAPK) activity is essential for pronuclear formation, but the precise timing and mechanism of decline are unknown. Here, we have measured the dynamics of MAPK pathway inactivation during fertilization of mouse eggs using novel chemiluminescent MAPK activity reporters...

... mitogen activated protein kinases (MAPK3 and MAPK1), but also suppressed TGFβ-induced activation of Smad2 by modulating Smad2 phosphorylation by MAPKs. These results suggest that FGF2 regulates LEC-specific gene expression and suppresses TGFβ signalling in LECs through Smad2 in a Ras–MAPK-dependent manner...

...Katarzyna M. Kowalczyk; Sonya Hartmuth; David Perera; Peter Stansfield; Janni Petersen Summary In all eukaryotes tight control of mitogen-activated protein kinase (MAPK) activity plays an important role in modulating intracellular signalling in response to changing environments. The fission yeast...

... ) 29 5 2012 © 2012. Published by The Company of Biologists Ltd 2012 Purinergic receptor Hemidesmosome β4 Integrin p90RSK Gαq MAPK ERK1/2 Migration Keratinocyte Hemidesmosomes (HD) are multiprotein complexes that promote stable adhesion of basal epithelial cells...

... reduced iNOS mRNA and protein expression in p14-deficient macrophages. This antimicrobial defense mechanism, relevant for Salmonella elimination within macrophages, is initiated by a plethora of signaling cascades, including MAPK ( Caivano, 1998 ; Vazquez-Torres et al., 2000b ) signaling pathways. Thus...

... of these findings for our understanding of RTK signaling specificity in different biological processes. * Author for correspondence ( gjcbmc@ibmb.csic.es ) © 2012. 2012 Capicua Cell proliferation CIC MAPK RTK signaling Transcriptional repression Tumor suppressor Receptor tyrosine kinase...

...-interacting protein-1 (JIP1) and kinase-D-interacting substrate of 220 kDa (Kidins220). KLC1ser460 is a predicted mitogen-activated protein kinase (MAPK) target site, and we show that extracellular-signal-regulated kinase (ERK) phosphorylates this residue in vitro. We also demonstrate that inhibition of ERK...

...@bristol.ac.uk ) 3 9 2010 © 2010. 2010 ERK MAPK Protein kinase C Epidermal growth factor Docking domain Nuclear localization High-content analysis A wide variety of extracellular signals activate the Raf–MEK–ERK pathway. In this cascade, Raf isoforms are recruited to the cell...

... evidence for the existence of ER microdomains that are involved in the regulation of BIT1 structure and trafficking, and identifies BIT1 as a negative regulator of the ERK-MAPK signaling pathway in the Golgi. * These authors contributed equally to this work ‡ Author for correspondence...

... protein kinase (MAPK) Sty1 (also known as Spc1). Here we show that `nutrient starvation' (complete withdrawal of nitrogen or leucine) blocks mitotic commitment by altering Sty1 signalling and that different degrees of Sty1 activation determine these differences in mitotic commitment decisions. Mammals...

... the inhibitors U0126 for mitogen-activated protein kinase (MAPK), and ZM447439 for Aurora kinase A and Aurora kinase B, we found that MAPK is required for phosphorylation of CPEB, whereas Aurora kinases are not. Furthermore, we do not detect active Aurora kinase A in early meiosis. By contrast, we observe...