... , A. and Lahav , G. ( 2012 ). Quantitative live cell imaging reveals a gradual shift between DNA repair mechanisms and a maximal use of HR in mid S phase . Mol. Cell 47 , 320 - 329 . 10.1016/j.molcel.2012.05.052 Kim , D. , Langmead , B. and Salzberg , S. L. ( 2015 ). HISAT...

... 1 2022 © 2022. Published by The Company of Biologists Ltd 2022 Summary: A description of RNA-assisted sequestration of TIA1 and other RNA-binding proteins by TDP-35 and live-cell imaging for dynamic sequestration of TDP-43. TDP-43 RNA-assisted sequestration RNA-binding...

... characterized, the spatiotemporal regulation of many selective autophagy processes, including aggrephagy, remains largely unexplored. Furthermore, because most live-cell imaging studies have so far focused on starvation-induced autophagy, little is known about the dynamics of aggrephagy. Here, we describe...

...Yiming Yu; Shige H. Yoshimura ABSTRACT Despite numerous recent developments in bioimaging techniques, nanoscale and live-cell imaging of the plasma membrane has been challenging because of the insufficient z -resolution of optical microscopes, as well as the lack of fluorescent probes...

.... Published by The Company of Biologists Ltd 2020 Summary: A summary of the various super-resolution microscopy techniques that are now part of the cell biologist's toolkit. Super-resolution microscopy Live-cell imaging Labeling Sample preparation Fluorescence microscopy allows...

... a simple novel non-invasive fluorescence microscopy-based approach to transiently impact molecular dynamics in order to visualise, dissect and study signalling pathways and cellular processes in living cells. Fig. 3. Image quality and live-cell imaging. (A) Micrographs of live cam1-YFP fission...

...), Rac1 activation is required for lamellipodial extension along the surface of IgG-Es. However, the significance of Rac1 deactivation in phagosome formation is poorly understood. Our live-cell imaging and electron microscopy revealed that RAW264 macrophages expressing a constitutively active Rac1 mutant...

... actin filaments, emphasizing the advantages and pitfalls of available tools to investigate F-actin not only in the cytoplasm, but also in the somatic cell nucleus. Actin dynamics Actin probes Live-cell imaging Nuclear actin Actin is one of the most abundant proteins in eukaryotic cells...

...: This article presents the preclinical utility of an automated in vitro imaging platform that enables quantitative single-cell spatiotemporal monitoring of the cell cycle. Automated microscopy DNA replication origin Drug synergy FUCCI Live-cell imaging Taxane The fluorescence ubiquitination...

.../cshperspect.a017459 Gross , S. M. and Rotwein , P. ( 2013 ). Live cell imaging reveals marked variability in myoblast proliferation and fate . Skelet. Muscle   3 , 10 . 10.1186/2044-5040-3-10 Gross , S. M. and Rotwein , P. ( 2015 ). Akt signaling dynamics in individual cells...

... factors, leptomycin B and PI-103 were diluted into supplemented FluoroBrite just before use. Live-cell imaging was conducted using an EVOS FL Auto microscope with a built-in stage top incubator maintained at 37°C and in 95% air, 5% CO 2 . Images were collected at 100× magnification using a 10× fluorite...

... or financial interests. 3 04 2014 28 01 2015 © 2015. Published by The Company of Biologists Ltd 2015 Sar1 Golgi dynamics Live-cell imaging Secretory pathway The Golgi in mammalian cells is a highly organized organelle that consists of several stacks of flattened, membrane...

... gene locus and the c887 control locus, were measured in cut3-477 and cut14-208 condensin mutant cells ( n >35). Wild-type and cut3-477 cells were cultured at the restrictive (36°C) temperature for 2 hours and subjected to live-cell imaging analysis within a temperature-controlled chamber...

..., perfused by a gravity-fed system (flow rate >5 ml/minute) with triggered valves that allowed the fast and reproducible exchange of solutions. For the physiological live-cell imaging experiments, lung slices were restrained in the perfusion chamber with a golden ring spanned with a sheet of nylon mesh...

... © 2013. Published by The Company of Biologists Ltd 2013 Islet β-cells Apoptosis Necrosis Live-cell imaging Fluorescence resonance energy transfer FRET For cell death assays, MIN6 and dispersed islet cells were seeded into 96-well plates and infected with lentiviral particles...

.... * These authors contributed equally to this work ‡ Author for correspondence ( okabe@biken.osaka-u.ac.jp ) 19 7 2012 © 2012. Published by The Company of Biologists Ltd 2012 Acrosome reaction Membrane fusion Protein trafficking Live-cell imaging IZUMO1 Sperm–egg fusion...

... phagocytosis in macrophages. Live-cell image analysis revealed that Rab35 was markedly concentrated at the membrane where IgG-opsonized erythrocytes (IgG-Es) are bound. Rab35 silencing by RNA interference (RNAi) or the expression of GDP- or GTP-locked Rab35 mutant drastically reduced the rate of phagocytosis...

... demonstrates that roGFP1-iL can be used to monitor real-time changes to the redox status in individual living cells. * Author for correspondence ( neil.bulleid@glasgow.ac.uk ) 24 3 2011 © 2011. 2011 Redox monitoring Disulphide formation Live-cell imaging Ero1 Peroxiredoxin IV...

... N. , Griffiths T. , Spiller D. G. , Oliver S. G. , White M. R. , et al . ( 2009 ). Information management for high content live cell imaging . BMC Bioinformatics 10 , 226 . Khabar K. S. , Siddiqui S. , Armstrong J. A. ( 1995 ). WEHI-13VAR: a stable...

... network (for details, see Windoffer et al., 2004 ; Woll et al., 2005 ). The goal of our study was to fill the existing gaps in these two hypotheses and to evaluate their contribution to KF-network dynamics. Using live-cell imaging, we discovered a protein-biosynthesis-independent multistep...