... lamellipodia defects in CK1α-depleted macrophages. Therefore, we conclude that basal phosphorylation of WAVE by CK1α protects it from premature ubiquitin-dependent degradation, thus promoting WAVE function in vivo . This article has an associated First Person interview with the first author of the paper...

.... and Watanabe , N. ( 2013 ). Can filament treadmilling alone account for the F-actin turnover in lamellipodia? Cytoskeleton (Hoboken) 70 , 179 - 190 . 10.1002/cm.21098 Moriyama , K. and Yahara , I. ( 2002 ). Human CAP1 is a key factor in the recycling of cofilin and actin...

...-activated receptor (uPAR) ( Vinik et al., 2015 ). HeLa cell spreading manifests itself primarily in the continuous extension of two types of membrane protrusion, filopodia and lamellipodia. We used time-lapse interference reflection microscopy (IRM) ( Barr and Bunnell, 2009 ) and differential...

...Indranil Ghosh; Raman K. Singh; Manjari Mishra; Shobhna Kapoor; Siddhartha S. Jana ABSTRACT Cells can adopt both mesenchymal and amoeboid modes of migration through membrane protrusive activities, namely formation of lamellipodia and blebbing. How the molecular players control the transition...

... this lamellipodia appeared, giving an apparent increase in bound receptors of up to 50%. Our findings show how parameters other than the specific protein–protein interaction can influence binding behavior at cell–cell contacts. * Authors for correspondence ( peter.jonsson@fkem1.lu.se ; victoria.junghans@fkem1...

...Yuka Ikeda; Katsuhisa Kawai; Akira Ikawa; Kyoko Kawamoto; Youhei Egami; Nobukazu Araki ABSTRACT Lamellipodia are sheet-like cell protrusions driven by actin polymerization mainly through Rac1, a GTPase molecular switch. In Fcγ receptor-mediated phagocytosis of IgG-opsonized erythrocytes (IgG-Es...

.... To study haptotaxis in response to gradients, we utilized microfluidic chambers to generate gradients of the ECM protein fibronectin, and imaged the cell migration response. Lamellipodia are fan-shaped protrusions that are common in migrating cells. Here, we define a new function for lamellipodia...

...Tadamoto Isogai; Rob van der Kammen; Daniela Leyton-Puig; Katarzyna M. Kedziora; Kees Jalink; Metello Innocenti ABSTRACT Protrusion of lamellipodia and ruffles requires polymerization of branched actin filaments by the Arp2/3 complex. Although regulation of Arp2/3 complex activity has been...

... to bind to Ena/VASP‐family proteins (referred to here as Ena/VASP), other regulators of actin polymerization, and Lpd plays a crucial role in advancing the lamellipodia in migrating cells ( Krause et al., 2004 ). We have found recently that the WAVE complex, bound to Pcdh17, interacts with Lpd and Ena...

... protrusions. Analysis of single lamellipodia showed that the concentration of ERC1a becomes evident soon after the protrusive activity starts, and that it is specifically localized behind the protruding lamellipodium. ERC1a-positive clusters disappear rapidly as the lamellipodium halts and starts to retract...

... accompanied by protrusion of membrane ruffles and lamellipodia. In two-dimensional migration, protrusion of these thin sheets of cytoplasm is considered relevant to both exploration of new space and initiation of nascent adhesion to the substratum. Lamellipodium formation can be potently stimulated by Rho...

... lamellipodia formation induced by IGF-I via association with PIP2 and beta-actin at membrane microdomains.   J. Cell. Physiol.   220 , 748 – 755 . 10.1002/jcp.21822 Yamashita   N. , Uchida   Y. , Ohshima   T. , Hira   S. , Nakamura   F. , Taniguchi   M. , Mikoshiba   K...

... and actin dynamics. In many cell types, CIP4 functions in vesicle formation, endocytosis and membrane tubulation. However, recent data indicate that CIP4 is also involved in protrusion in some cell types, including cancer cells (lamellipodia and invadopodia) and neurons (ribbed lamellipodia and veils...

... is responsible for the response. Stable lamellipodial extensions formed at the cathodal sides of wild-type cells at the start of galvanotaxis; these were absent in the ENaC knockout keratinocytes, suggesting that ENaC mediates galvanotaxis by generating stable lamellipodia that steer cell migration. We provide...

... lamellipodium wandering around the cell perimeter, and increased lamellipodial protrusion rates. These protrusive and polarity-stabilizing effects also enabled spreading SMCs to undergo bipolar elongation to an extent not typically observed in vitro . Nampt was found to localize to lamellipodia and fluorescence...

... branch junctions in protruding and treadmilling lamellipodia are not concentrated at the front as previously supposed, but link actin filament subsets in which there is a continuum of distances from a junction to the filament plus ends, for up to at least 1 μm. When branch sites were observed closely...

... the molecular mechanism by which Gal-3 stimulates cell migration. We demonstrate here that exogenous Gal-3, but not Gal-1 or Gal-8, promotes cell scattering and formation of lamellipodia in human corneal epithelial cells in a β-lactose-inhibitable manner. α3β1 integrin was identified as the major Gal-3-binding...

... filaments within these lamellipodia were shorter and more highly branched, suggesting that filament capping activity was increased. Conversely, membrane targeting of Ena/VASP protein led to longer filaments with fewer branches. Collectively, these data suggested that Ena/VASP proteins were `anti-capping...

...-VASP constructs, we found that NO produced rapid retraction of lamellipodia together with cell rounding that was dependent on guanylate cyclase and type II cGMP-dependent protein kinase. In cells expressing a mutant VASP (Ser239Ala) lacking the site preferentially phosphorylated by this kinase, NO had...

... Lamellipodia Cell migration is an essential property of cells engaged in embryogenesis, inflammation, wound healing and tumor metastasis. Motility toward a source of chemoattractant depends on localized extension of actin-rich protrusions, such as lamellipodia. Studies with metastatic rat carcinoma...