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Keywords: Knocksideways
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Journal Articles
J Cell Sci (2021) 134 (3): jcs255794.
Published: 1 February 2021
... proteins from the spindle to mitochondria. GTSE1 TACC3 Clathrin Knocksideways Mitosis Mitotic spindle Knocksideways is a method to acutely and inducibly relocalize a protein to mitochondria in order to inactivate that protein ( Robinson et al., 2010 ). In the original method, the target...
Includes: Supplementary data
Journal Articles
J Cell Sci (2020) 133 (15): jcs246033.
Published: 3 August 2020
... the glucose transporter GLUT1 (also known as SLC2A1). The role of retromer in the retrograde sorting of the cation-independent mannose 6-phosphate receptor (CI-MPR, also known as IGF2R) from endosomes back to the trans -Golgi network remains controversial. Here, by applying knocksideways technology, we...
Includes: Supplementary data
Journal Articles
J Cell Sci (2019) 132 (21): jcs234955.
Published: 6 November 2019
... additional tags, functionalized nanobodies that bind GFP can be used to extend the functionality of the GFP tag. We developed functionalized nanobodies, which we termed ‘dongles’, that could add, for example, an FKBP tag to a GFP-tagged protein of interest, enabling knocksideways experiments in GFP knock...
Includes: Supplementary data
Journal Articles
J Cell Sci (2013) 126 (9): 2102–2113.
Published: 1 May 2013
... and maintenance roles for a given protein is difficult. Most experimental methods for protein inactivation are slow and therefore affect both assembly and maintenance. Here, we have used ‘knocksideways’ to rapidly (∼5 minutes) and specifically remove TACC3–ch-TOG–clathrin non-motor complexes from kinetochore...
Includes: Supplementary data