... the molecular mechanisms that regulate basal lipolysis. Here, we describe a mechanism whereby autocrine purinergic signalling and constitutive P2Y 2 receptor activation suppresses basal lipolysis in primary human in vitro -differentiated adipocytes. We found that human adipocytes possess cytoplasmic Ca 2+ tone...

...Julie Perroud; Laurent Bernheim; Maud Frieden; Stephane Koenig ABSTRACT Ca 2+ signaling plays a key role during human myoblast differentiation. Among Ca 2+ -sensitive pathways, calcineurin is essential for myoblast differentiation and muscle regeneration. Nuclear factor of activated T-cell (NFAT...

...Manabu Ohyama; Tetsuro Kobayashi; Takashi Sasaki; Atsushi Shimizu; Masayuki Amagai Summary The dermal papilla (DP) plays pivotal roles in hair follicle morphogenesis and cycling. However, characterization and/or propagation of human DPs have been unsatisfactory because of the lack of efficient...

..., chromosomes are organized in territories that are radially positioned in the nucleus. However, it remains unclear whether chromosomes are organized relative to each other. Here, we examine the nuclear arrangement of 10 chromosomes in human epithelial cancer cells by three-dimensional FISH analysis. We show...

... for correspondence (e-mail: claire.harper@liv.ac.uk ) 22 4 2008 © The Company of Biologists Limited 2008 2008 Acrosome Exocytosis Human Sperm Over 80 million people worldwide experience infertility and over one third of infertility cases are due to male factors, i.e. sperm dysfunction...

...C. Christodoulides; M. Laudes; W. P. Cawthorn; S. Schinner; M. Soos; S. O'Rahilly; J. K. Sethi; A. Vidal-Puig Secretion of Wnts by adipose cells has an important role in the control of murine adipogenesis. We present the first evidence that a Wnt antagonist, Dickkopf 1 (Dkk1), is secreted by human...

..., human gamete fusion remains largely unknown. We now show that an anti-α6 integrin mAb (GoH3) strongly inhibited human sperm-egg fusion in human zona-free eggs. Furthermore, a mAb directed against CD151, a tetraspanin known to associate with α6β1, partially inhibited sperm-egg fusion. By contrast...

.... Price 14 3 2005 © The Company of Biologists Limited 2005 2005 Chromatin immunoprecipitation Human Replication origins Nascent DNA abundance Ku protein Haploinsufficient DNA replication is regulated at the level of initiation, and more specifically, at the level of pre...

... are human homologues of the only known protein mediators of mitochondrial fusion, the Drosophila Fzo GTPase and Saccharomyces cerevisiae yFzo1p. Although the Mfn1 and Mfn2 genes were broadly expressed, the two genes showed different levels of mRNA expression in different tissues. Two Mfn1 transcripts were...

...Daniela Uberti; Teresina Carsana; Enza Bernardi; Luigi Rodella; Piergiovanni Grigolato; Cristina Lanni; Marco Racchi; Stefano Govoni; Maurizio Memo In this study, we evaluated the response of different human skin fibroblast cultures obtained from eight probable Alzheimer's disease patients...

...Harmeet Malhi; Adil N. Irani; Singh Gagandeep; Sanjeev Gupta The liver can regenerate itself through the progenitor cells it harbors. Here we demonstrate isolation of epithelial progenitor/stem cells from the fetal human liver, which contains a large number of hepatoblasts. Progenitor liver cells...

..., probably by preventing anti-CD95-induced translocation of CD95 from intracellular stores to the cell surface. 23 4 2001 © The Company of Biologists Limited 2001 2001 CD95-induced cell death Pancreatic cancer Human Immune escape Extracellular signals that are transduced...

...Ansgar Santel; Margaret T. Fuller ABSTRACT Although changes in mitochondrial size and arrangement accompany both cellular differentiation and human disease, the mechanisms that mediate mitochondrial fusion, fission and morphogenesis in mammalian cells are not understood. We have identified two...

... related to TbNOG1 are present in yeast and metazoa, and related G domains are found in bacteria. We show that NOG1 proteins in humans and Saccharomyces cerevisae are also nucleolar. The S. cerevisae NOG1 gene is essential for cell viability, and mutations in the predicted G motifs abrogate function...

.... Affinity-purified anti-zonula occludens toxin antibodies and the Ussing chamber assay were used to screen for one or more mammalian zonula occludens toxin analogues in both fetal and adult human intestine. A novel protein, zonulin, was identified that induces tight junction disassembly in non-human primate...

...Robert G. Nagele; Theresa Freeman; Lydia McMorrow; Zabrina Thomson; Kelly Kitson-Wind; Hsin-yi Lee ABSTRACT The relative spatial positioning of chromosomes 7, 8, 16, X and Y was examined in nuclei of quiescent (noncycling) diploid and triploid human fibroblasts using fluorescence in situ...

... human limb buds from the beginning of primary fibre formation throughout subsequent development and post-natal growth. Four phenotypically distinct types of myoblasts were identified on the basis of their expression of desmin, myogenin and myosin heavy chain isoforms (MyHC), and after 5 and 20 divisions...

... epithelial sheets. We show here that a much used commercial human preparation contains laminin-10 (α5β1γ1), some laminin-11 (α5β2γ1), but no laminin-1. Moreover, the laminin-10/11 mixture was found to be a strong adhesive for two human cell lines derived from epithelia. Antibodies against integrin β1, α6...

...H. Scherthan; R. Eils; E. Trelles-Sticken; S. Dietzel; T. Cremer; H. Walt; A. Jauch ABSTRACT The three-dimensional morphology and distribution of human chromosomes 3 were studied in nuclei of spermatogonia and spermatocytes I from formaldehyde-fixed human testis sections. Chromosome arms...

... unidentified) function during male germ cell development. In order to address this function, we have developed new antibody reagents to immunolocalise RBM in the different cell types in the human testis. We find that RBM has a different expression profile from its closest homologue hnRNPG. Despite its...