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Keywords: Fluorescence microscopy
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Journal Articles
J Cell Sci (2022) 135 (3): jcs258994.
Published: 10 February 2022
... cellular structures ( Caicedo et al., 2019 ). Fluorescence microscopy is a commonly used technique in cell biology for determining the spatial distribution and abundance of target proteins in cells. It relies on the use of highly specific labels to visualise the cellular components of interest...
Includes: Supplementary data
Journal Articles
J Cell Sci (2020) 133 (4): jcs242834.
Published: 21 February 2020
... and phototoxicity. A workflow is presented to minimize the negative effects of IO for live imaging. Fluorescence microscopy Phototoxicity Cell migration Mitochondrial dynamics Microtubule dynamics Adhesion dynamics Fluorescence microscopy provides a convenient, selective and sensitive way...
Includes: Supplementary data
Journal Articles
In collection:
Imaging
J Cell Sci (2018) 131 (3): jcs211847.
Published: 8 February 2018
... , 3993 - 4003 . 10.1529/biophysj.103.038422 Dickinson , M. E. , Bearman , G. , Tille , S. , Lansford , R. and Fraser , S. E. ( 2002 ). Multi-spectral imaging and linear unmixing add a whole new dimension to laser scanning fluorescence microscopy . BioTechniques   31...
Journal Articles
J Cell Sci (2017) 130 (13): 2185–2195.
Published: 1 July 2017
...., 2005 ). HER-911 cells have a perinuclear microtubule network, which is accessible to analyses by super-resolution fluorescence microscopy. To obtain super-resolution, we used the gated-stimulated emission depletion microscopy (gSTED) in 3D mode, with spatial resolution of ∼135 nm in both x - y and z...
Includes: Supplementary data
Journal Articles
J Cell Sci (2014) 127 (9): 1899–1910.
Published: 1 May 2014
... Ltd 2014 JAK-STAT signalling STAT3 Dimerization Inflammatory hepatocellular adenoma Hyper-IgE syndrome Fluorescence microscopy FRET The signal transducers and activators of transcription (STATs) are dual function proteins which receive an activating signal from the cell surface...
Journal Articles
J Cell Sci (2013) 126 (12): 2692–2703.
Published: 15 June 2013
... stabilization in the cell periphery, we investigated the peripheral trafficking itineraries and kinetics of wild type (WT) and F508del in living cells using high-speed fluorescence microscopy together with fluorogen activating protein detection. We directly visualized internalization and accumulation of CFTR WT...
Includes: Supplementary data
Journal Articles
J Cell Sci (2013) 126 (4): 879–888.
Published: 15 February 2013
... subunit of SOCE channels, Orai1. Fluorescence microscopy revealed that NAGly did not affect STIM1 oligomerization, STIM1 clustering, or the colocalization of STIM1 with Orai1, which were induced by Ca 2+ depletion of the endoplasmic reticulum. In contrast, independently from its slow depolarizing effect...
Includes: Supplementary data
Journal Articles
In collection:
Lipid Biology
J Cell Sci (2011) 124 (22): 3894–3904.
Published: 15 November 2011
...). In accordance with data obtained with fluorescence microscopy, lipid droplets in the small bud always appeared closely associated with the ER membrane extending from the mother cell to the bud tip ( Fig. 4a,b ). Interactions of lipid droplets with ER membrane were found to be independent of the lipid droplet...
Includes: Multimedia, Supplementary data
Journal Articles
J Cell Sci (2011) 124 (5): 727–734.
Published: 1 March 2011
... cytoskeleton Intracellular Ca 2+ SNARE protein Fluorescence microscopy The function of the F-actin cortex in secretion remains controversial, particularly because it has been assigned various roles, such as being a barrier, being involved in transportation or even being a scaffold in neuronal...
Includes: Multimedia, Supplementary data
Journal Articles
J Cell Sci (2009) 122 (23): 4296–4302.
Published: 1 December 2009
... of drug addition is a deterministic decision. * Author for correspondence ( simon@rockefeller.edu ) 15 9 2009 © The Company of Biologists Limited 2009 2009 Fluorescence microscopy Protease Caspase Lineage Epigenetics Apoptosis is a form of cell death that eliminates...
Includes: Multimedia, Supplementary data
Journal Articles
J Cell Sci (2008) 121 (22): 3757–3769.
Published: 15 November 2008
... of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA 12 8 2008 © The Company of Biologists Limited 2008 2008 Endocytosis Axolemma Synaptic vesicle Trafficking Fluorescence microscopy In the nervous system, axons are guided to their specific targets...
Includes: Multimedia, Supplementary data
Journal Articles
J Cell Sci (2006) 119 (15): 3098–3106.
Published: 1 August 2006
... in the mitochondrial fission process. * Present address: Institute for Molecular Biophysics, The Jackson Laboratory, 600 Main Street, Bar Harbor, ME 04609, USA 27 4 2006 © The Company of Biologists Limited 2006 2006 Actin Dnm1 Dynamin Fluorescence microscopy Mitochondrial fission Polarity...
Includes: Supplementary data
Journal Articles
J Cell Sci (2005) 118 (15): 3277–3288.
Published: 1 August 2005
... The Company of Biologists Limited 2005 2005 Transcriptional regulation Prolactin Nuclear co-repressor SMRT Nuclear structure Green fluorescent protein Fluorescence microscopy Pit-1 Coactivator and co-repressor protein complexes control gene expression by modifying the acetylation states...
Journal Articles
J Cell Sci (2005) 118 (9): 1799–1809.
Published: 1 May 2005
... dynamics of this phenomenon is limited. In this report, the effect of the activation of H-Ras on its microdomain localization was studied by single-molecule fluorescence microscopy. Individual human H-Ras molecules fused to the enhanced yellow fluorescent protein (eYFP) were imaged in the dorsal plasma...
Journal Articles
J Cell Sci (2003) 116 (10): 2005–2014.
Published: 15 May 2003
.... ‡ Author for correspondence (e-mail: sjakobs@gwdg.de ) 11 2 2003 © The Company of Biologists Limited 2003 2003 Saccharomyces cerevisiae Fluorescence microscopy Glucose repression Membrane fission Organelle morphology Mitochondria carry out numerous essential metabolic...
Includes: Multimedia, Supplementary data
Journal Articles
J Cell Sci (1990) 96 (4): 721–730.
Published: 1 August 1990
... period. This apparent difference in the ability of cells and protoplasts to internalise FITC—dextrans was confirmed using samples of both commercial and purified 20K FITC—dextran as putative endocytotic probes. Both confocal and conventional fluorescence microscopy of FITC—treated cells have shown...
Journal Articles
J Cell Sci (1989) 93 (3): 565–570.
Published: 1 July 1989
...YASUKO HAYASHI; KATSUMI UEDA The shape of mitochondria and the number of mitochondrial nucleoids in Euglena cells were examined throughout the cell cycle by fluorescence microscopy. Both photoheterotrophic and heterotrophic cells contained a network of mitochondria that did not divide...