...Aaron R. Tipton; Gary J. Gorbsky ABSTRACT The microtubules of the mitotic spindle mediate chromosome alignment to the metaphase plate, then sister chromatid segregation to the spindle poles in anaphase. Previous analyses of spindle microtubule kinetics utilizing fluorescence dissipation after...

...Christopher Estell; Emmanouela Stamatidou; Sarah El-Messeiry; Andrew Hamilton ABSTRACT Although mitochondrial translation produces only 13 proteins, we show here how this process can be visualised and detected in situ by fluorescence microscopy with a simple, rapid and inexpensive procedure using...

...Eugenio Gallo; Jonathan W. Jarvik ABSTRACT A novel bi-partite fluorescence platform exploits the high affinity and selectivity of antibody scaffolds to capture and activate small-molecule fluorogens. In this report, we investigated the property of multi-selectivity activation by a single antibody...

.... ( 2007 ). Multicolor super-resolution imaging with photo-switchable fluorescent probes . Science   317 , 1749 - 1753 . 10.1126/science.1146598 Berika , M. and Garrod , D. ( 2014 ). Desmosomal adhesion in vivo . Cell Commun. Adhes.   21 , 65 - 75 . 10.3109/15419061.2013.876018...

...Liang Gao; Alejandro Garcia-Uribe; Yan Liu; Chiye Li; Lihong V. Wang ABSTRACT We present a generic sub-diffraction-limited imaging method – photobleaching imprinting microscopy (PIM) – for biological fluorescence imaging. A lateral resolution of 110 nm was measured, more than a twofold improvement...

... to contribute to future solutions. * Author for correspondence ( timm.schroeder@bsse.ethz.ch ) © 2013. Published by The Company of Biologists Ltd 2013 Biosensors Fluorescence Hardware Single-cell imaging Software Time-lapse microscopy Organisms are composed of vast numbers...

.... It is especially true in cells where the spatiotemporal distribution of Tau–MT interactions is unknown. Using Förster resonance energy transfer (FRET), we showed that the Tau–MT interaction was distributed along MTs in periodic hotspots of high and low FRET intensities. Fluorescence recovery after photobleaching...

...Alexa L. Mattheyses; Sanford M. Simon; Joshua Z. Rappoport Total internal reflection fluorescence (TIRF) microscopy can be used in a wide range of cell biological applications, and is particularly well suited to analysis of the localization and dynamics of molecules and events near the plasma...

... for correspondence ( maddy.parsons@kcl.ac.uk ) © 2010. 2010 Cell adhesion Fluorescence Focal adhesion Live imaging Microscopy Migration Several different types of adhesions are formed by cells following engagement with the extracellular matrix (ECM). These structures are classified on the basis...

... * Qdot tracking † antibody-coated 0.5 μm latex beads ‡ Cy5 or Alexa-Fluor-647-labeled antibodies § 40-nm-gold-conjugated antibodies ¶ antibody-coupled 50-100 nm fluorescent spheres Fig. 3. Analysis of GFP-Kv2.1-loopBAD ΔC mobility on the cell surface. HEK cells expressing...

... Fluorescence Membrane distribution Western blots were performed according to standard protocols. After electrotransfer and blocking (1 hour at room temperature in 10 mM Tris pH 7.9, 150 mM NaCl, 0.1% Tween 20, 3% BSA), the blot was incubated for 12 hours at 4°C in blocking buffer with monoclonal...

... fluorescence imaging measurements. Co-transfected COS7 cells as well as normal or serum starved A431 cells were grown on 22 mm cover slips. A full 48 hours after transfecting COS7 cells or 72 hours after serum-starvation of A431 cells, cells were stimulated or mock stimulated with BMP-2 for 2.5 hours and fixed...

...Jeffrey M. Levsky; Robert H. Singer Fluorescence in situ hybridization (FISH), the assay of choice for localization of specific nucleic acids sequences in native context, is a 20-year-old technology that has developed continuously. Over its maturation,various methodologies and modifications have...

...Pamela K. Hanson; Althea M. Grant; J. Wylie Nichols At low temperature, the short-chain fluorescent-labeled phospholipids,1-myristoyl-2-[6-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)aminocaproyl]-phosphatidylcholine (M-C 6 -NBD-PC) and its phosphatidylethanolamine analog, M-C 6 -NBD-PE, are internalized...

...-tails promise to give new insights into the stability and lifetime of membrane domains. © The Company of Biologists Limited 1993 1993 plasma membrane lateral diffusion fluorescence FPR domains Journal o f Cell Science, Supplement 17, 165-169 (1993) Printed in Great Britain © The Company...