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Keywords: Fission yeast
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J Cell Sci (2023) 136 (19): jcs260930.
Published: 3 October 2023
... the maintenance of INM homeostasis remain poorly understood. In this study, we explored the degradation mechanisms of the INM protein Bqt4 in the fission yeast Schizosaccharomyces pombe . We have previously shown that Bqt4 interacts with the transmembrane protein Bqt3 at the INM and is degraded in the absence...
Includes: Supplementary data
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Membrane trafficking
J Cell Sci (2023) 136 (12): jcs260598.
Published: 27 June 2023
... distribution of Pkd2 by promoting its internalization and preventing its clustering in the eisosome, a caveolae-like membrane compartment. The domains of Pkd2 and their functions are conserved in other fission yeast species. We conclude that both extracellular and cytoplasmic domains of Pkd2 are crucial...
Includes: Supplementary data
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J Cell Sci (2023) 136 (6): jcs260769.
Published: 20 March 2023
.... Here, we report the successful implementation of the CRISPR-Cas13d system in fission yeast for RNA knockdown. A high effectiveness of the CRISPR-Cas13d system was ensured by using an array of CRISPR RNAs (crRNAs) that are flanked by two self-cleaving ribozymes and are expressed from an RNA polymerase...
Includes: Supplementary data
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Series: REVIEW COMMONS TRANSFER
J Cell Sci (2021) 134 (24): jcs259315.
Published: 30 December 2021
... biliverdin (BV) as a chromophore. Here, we report that phycocyanobilin (PCB) functions as a brighter chromophore for iRFP than BV, and that biosynthesis of PCB allows live-cell imaging with iRFP in the fission yeast Schizosaccharomyces pombe . We initially found that fission yeast cells did not produce BV...
Includes: Supplementary data
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J Cell Sci (2021) 134 (6): jcs253021.
Published: 26 March 2021
...Sanju Ashraf; Ye Dee Tay; David A. Kelly; Kenneth E. Sawin ABSTRACT Movement of the cell nucleus typically involves the cytoskeleton and either polymerization-based pushing forces or motor-based pulling forces. In the fission yeast Schizosaccharomyces pombe , nuclear movement and positioning...
Includes: Supplementary data
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