... established from primary hepatocytes of Pkdcc knockout mice produced a secretome, which promoted their own proliferation in 3D spheroids and proliferation of cultured fibroblasts. In vivo , Pkdcc knockout mice developed liver steatosis with signs of inflammation and perivascular fibrosis upon aging, combined...

... cooperate in kidney collecting duct development, we deleted α3 and α6 in the developing ureteric bud. The collecting system of the double knockout phenocopied the α3 integrin conditional knockout. However, with age, the mice developed severe inflammation and fibrosis around the collecting ducts, resulting...

.... eIF5A Translation Collagen ER stress Fibrosis Ministerio de Economía y Competitividad http://dx.doi.org/10.13039/501100003329 BFU2016–77728-C3–3-P Generalitat Valenciana http://dx.doi.org/10.13039/501100003359 AICO2020/086 Ministerio de Ciencia, Innovación y...

...Marielle Saclier; Sabrina Ben Larbi; Ha My Ly; Eugénie Moulin; Rémi Mounier; Bénédicte Chazaud; Gaëtan Juban ABSTRACT Duchenne muscular dystrophy is a genetic muscle disease characterized by chronic inflammation and fibrosis mediated by a pro-fibrotic macrophage population expressing pro...

... by tissue labeling with a collagen-hybridizing peptide. Together, these results implicate DRD1 agonism in fibroblast-mediated collagen clearance, suggesting an important role for this mechanism in fibrosis resolution. This article has an associated First Person interview with the first author of the paper...

... wound granulation tissue and, accordingly, named these cells ‘myofibroblasts’. Now, myofibroblasts are not only recognized for their physiological role in tissue repair but also as cells that are key in promoting the development of fibrosis in all organs. In this Cell Science at a Glance...

... Summary: A Review discussing the increasingly clear data showing that atypical basement membranes are quite prevalent and that even typical planar basement membranes harbor a lot of diversity that we do not comprehend yet. Collagen IV Drosophila Extracellular matrix Fibrosis Integrin Tissue...

...Dakota L. Jones; Andrew J. Haak; Nunzia Caporarello; Kyoung M. Choi; Zhenqing Ye; Huihuang Yan; Xaralabos Varelas; Tamas Ordog; Giovanni Ligresti; Daniel J. Tschumperlin ABSTRACT Tissue fibrosis is a chronic disease driven by persistent fibroblast activation that has recently been linked...

... constitutively active PDGFRα mutations promotes their activation and muscle fibrosis, negatively affecting skeletal muscle regeneration ( Ieronimakis et al., 2016 ). Furthermore, treatment with crenolanib and nilotinib (two potent PDGFRα inhibitors) reduces FAP activation and fibrosis during chronic muscle...

... implantation in a translucent zebrafish model and compare with an acute wound response. We observe inflammation extending from the suture margins, correlating with subsequent avascular and fibrotic encapsulation zones: sutures that induce more inflammation result in increased zones of avascularity and fibrosis...

... and differentiation of lens epithelial cells that could contribute to vision loss after cataract surgery. Lens TGFβ Epithelial-mesenchymal transition Fibrosis Cataract The lens consists of a monolayer of epithelial cells on its anterior face and the crystallin-rich lens fiber cells, both encased...

...; Robert Vassallo; Daniel J. Tschumperlin ABSTRACT Myofibroblasts play key roles in wound healing and pathological fibrosis. Here, we used an RNAi screen to characterize myofibroblast regulatory genes, using a high-content imaging approach to quantify α-smooth muscle actin stress fibers in cultured human...

... disordered and stiff extracellular matrix (ECM) leading to fibrosis. Fibronectin immobilizes latent TGF-β-binding protein-1 (LTBP-1) and thus stores TGF-β1 in the ECM. Because the ED-A fibronectin splice variant is prominently expressed during fibrosis and supports myofibroblast activation, we investigated...

.... , Waikar , S. S. , et al. ( 2016 ). ADAM17 substrate release in proximal tubule drives kidney fibrosis . JCI. Insight.   1 , e87023 . 10.1172/jci.insight.87023 Kleino , I. , Järviluoma , A. , Hepojoki , J. , Huovila , A. P. and Saksela , K. ( 2015 ). Preferred SH3...

... on alpha5beta1 integrins to strengthen adhesion to fibronectin . Nat. Commun.   8 , 14348 . 10.1038/ncomms14348 Bomberger , J. M. , Barnaby , R. L. and Stanton , B. A. ( 2009 ). The deubiquitinating enzyme USP10 regulates the post-endocytic sorting of cystic fibrosis transmembrane...

... also influences the level and activity of YAP1 in CAFs exposed to a stiff matrix. This work describes a mechanism whereby increased tumor fibrosis can perpetuate activation of CAFs to sustain tumor fibrosis and promote tumor metastasis through regulation of SNAIL1 protein level and activity. Human...

.... , Fox , D. A. , Khanna , D. , Larsen , S. D. and Neubig , R. R. ( 2014 ). Targeting the myofibroblast genetic switch: inhibitors of myocardin-related transcription factor/serum response factor-regulated gene transcription prevent fibrosis in a murine model of skin injury . J...

... in the extracellular matrix (ECM) of load-bearing tissues such as tendons and cartilage. It provides adaptor functions by bridging different ECM structures. We have previously shown that COMP is also a constitutive component of healthy human skin and is strongly induced in fibrosis. It binds directly and with high...

..., their roles in physiological and pathological settings with special emphasis on wound healing, fibrosis and tumor–stroma interactions, and include a discussion of the most recently identified newcomers to this subfamily – integrins α10β1 and α11β1. On the basis of our current understanding, collagen-binding...

...Manish K. Rana; Jyoti Srivastava; Michael Yang; Christopher S. Chen; Diane L. Barber ABSTRACT Increased production and assembly of extracellular matrix proteins during transdifferentiation of epithelial cells to a mesenchymal phenotype contributes to diseases such as renal and pulmonary fibrosis...