... pathways that each differ in their efficiency to generate releasable synaptic vesicles, we used the dye FM1-43 to track vesicle recycling, and to determine whether nerve terminals use multiple pathways of endocytosis. We identified two types of synaptic boutons in cultured cerebellar granule cells...

... esculenta . Ca 2+ imaging was done on Rana pipiens and the FM1-43 study was carried out on Bufo marinus (School of Biomedical Sciences Teaching Facility, University of Queensland, Australia). Adult frogs ( R. pipiens ) were anesthetized in a 0.1% tricaine methane sulfonate solution and killed by double...

...Roslyn A. Bauer; Ruth L. Overlease; Janet L. Lieber; Joseph K. Angleson We have used fluorescence imaging of individual exocytic events in combination with immunogold electron microscopy and FM1-43 photoconversion to study the stimulus-dependent recycling of dense core vesicle content in isolated...

... of Biologists Limited 2002 2002 Membrane capacitance FM1-43 Exocytosis Endocytosis SNARE proteins Trafficking Chloride channel Voltage clamp It is not surprising that many layers of regulation exist for ubiquitous proteins such as ion channels as they play central roles in inter...