... . However, the underlying molecular pathways were still elusive. Here, we report the critical roles of the filamentous actin (F-actin) network and actin-binding protein cortactin in RSV infection. We found that RSV infection causes F-actin depolymerization in 16HBE cells, and that stabilizing the F-actin...

... docking, but also mistargeting of SNARE protein syntaxin1 and an abnormally dense submembrane F-actin network. Here, we tested the contribution of both these phenomena to docking and secretion defects in MUNC18-1-deficient MCCs. We show that an abnormal F-actin network and syntaxin1 targeting defects...

...-9568.2005.04342.x Van Bommel , B. , Konietzny , A. , Kobler , O. , Bär , J. and Mikhaylova , M. ( 2019 ). F-actin patches associated with glutamatergic synapses control positioning of dendritic lysosomes . EMBO J.   38 , e101183 .   10.15252/embj.2018101183   Vlachos...

... to cell cycle progression in a pseudostratified epithelium. Here, we provide three levels of evidence showing that the cells from mammalian neuroepithelium undergoes apical constriction in a Rock-dependent manner: (1) apical F-actin localisation is lost, and (2) neuroepithelial apical areas are larger...

... that cortically localized mitochondria are abundant in rodent beta cells, and that these mitochondria redistribute towards the cell interior following depolarization. The redistribution requires Ca 2+ -induced remodeling of the cortical F-actin network. Using light-regulated motor proteins, we increased...

... feature of epithelial and polarised cells. This Review presents the dynamics of cortical domains in early Drosophila embryos and discusses underlying mechanisms for formation of cortical domains. Drosophila F-actin Cortical compartment Cortical domain Epithelia Below the plasma...

.... In contrast, cells transfected with PIP4K2a, which catalyzes PtdIns(4,5)P 2 production from PtdIns(5)P, increased phagocytosis. Fluorescent TMEM55a transfected into Raw264.7 cells was found to mostly localize to the phagosome. The accumulation of PtdIns(4,5)P 2 , PtdIns(3,4,5)P 3 and F-actin on the phagocytic...

... experiments showed that endogenous RhoC is transiently activated during phagosome formation. Notably, actin-driven pseudopod extension, which is required for the formation of phagocytic cups, was severely impaired in cells expressing the constitutively active mutant RhoC-G14V, which induced abnormal F-actin...

... of crosslinkers, have a critical role in integrating these different cytoskeletal networks. Spectraplakin genes give rise to a variety of isoforms that have distinct functions. Importantly, all spectraplakin isoforms are uniquely able to associate with all three elements of the cytoskeleton, namely, F-actin...

.... In Dictyostelium , it accumulates on lysosomes as they mature and is removed, together with the WASH complex, shortly before the postlysosomes are exocytosed. WASH-generated F-actin is required for correct subcellular localization; in WASH complex mutants, and immediately after latrunculin treatment, Mroh1...

... include the recognition and adhesion of myoblasts, and the formation of branched F-actin by the Arp2/3 complex at the site of cell–cell contact. At the ultrastructural level, these events are reflected by the appearance of finger-like protrusions and electron-dense plaques that appear prior to fusion...

... the branching point to the terminal end, but did not directly correlate with the presence of K5H. By contrast, F-actin signal values in arbor terminals correlated spatiotemporally with K5H, and inhibition of actin turnover prevented retraction. Results from the present study reveal a new system mediated...

... VEGFA-induced HRMVEC F-actin stress fiber formation, migration, proliferation, sprouting and tube formation. In accordance with these observations, hypoxia induced Pak1 and cofilin phosphorylation with p38MAPKβ being downstream to Pak1 and upstream to cofilin in mouse retina. Furthermore, Pak1...

... for 20 min and F-actin was stained with Alexa555–phalloidin (0.02 U/ml in PBS) for 1 h. The cells were mounted in non-hardening Vectashield mounting medium (Vector Laboratories, Burlingame, CA) immediately prior to microscopy. 3D-SIM images were acquired using a DeltaVision OMX V4/Blaze system (GE...

... acetylation sites are required for rescue of F-actin accumulation and focal adhesion maturation in Net1 knockout MEFs. These data indicate that Net1A acetylation regulates its subcellular localization to impact on RhoA activity and actin cytoskeletal organization. * Author for correspondence...

.... 25 02 2014 1 09 2014 © 2014. Published by The Company of Biologists Ltd 2014 Testis rpS6 Blood–testis barrier Spermatogenesis Seminiferous epithelial cycle Sertoli cells Germ cell transport F-actin Ectoplasmic specialization Tight junction In the mammalian testis...

... not been well studied. Here, we show that tricellulin (also known as MARVELD2), which is localized at tricellular contacts, regulates F-actin organization through Cdc42. Tricellulin-knockdown epithelial cells exhibit irregular polygonal shapes with curved cell borders and impaired organization of F-actin...

... and as an integrator to orient cell division. Recent advances in live imaging revealed novel pools and roles of F-actin in somatic cells and in oocytes. In somatic cells, cytoplasmic F-actin is involved in spindle architecture and positioning. In starfish and mouse oocytes, newly discovered meshes of F-actin control...

...Saïda Abounit; Chiara Zurzolo Tunneling nanotubes (TNTs) represent a subset of F-actin-based transient tubular connections that allow direct communication between distant cells. Recent studies have provided new insights into the existence of TNTs in vivo, and this novel mechanism of intercellular...

... by interference of the mutant with the content and organisation of F-actin at the junctions. A role for POF1B in the regulation of the actin cytoskeleton was further verified by shRNA silencing of the endogenous protein in human intestinal Caco-2 cells. Taken together, these data indicate that localisation...