...Franziska Lehne; Sven Bogdan ABSTRACT Exocytosis is a fundamental cellular process by which cells secrete cargos from their apical membrane into the extracellular lumen. Cargo release proceeds in sequential steps that depend on coordinated assembly and organization of an actin cytoskeletal network...

...Hanna C. A. Lammertse; Alessandro Moro; Ingrid Saarloos; Ruud F. Toonen; Matthijs Verhage ABSTRACT MUNC18-1 (also known as syntaxin-binding protein-1, encoded by Stxbp1 ) binds to syntaxin-1. Together, these proteins regulate synaptic vesicle exocytosis and have a separate role in neuronal...

... extracellular polyP inhibits exocytosis of undigested or partially digested nutrients. PolyP decreases plasma membrane recycling and apparent cell membrane fluidity, and this requires the G protein-coupled polyP receptor GrlD, the polyphosphate kinase Ppk1 and the inositol hexakisphosphate kinase I6kA. PolyP...

...Cristina Escrevente; Liliana Bento-Lopes; José S. Ramalho; Duarte C. Barral ABSTRACT Lysosomes are dynamic organelles, capable of undergoing exocytosis. This process is crucial for several cellular functions, namely plasma membrane repair. Nevertheless, the molecular machinery involved...

... place across the Golgi. GEF Golgi RAB GTPase Exocytosis Multisubunit tethering complex Transport protein particle (TRAPP) complexes regulate membrane traffic, acting as guanine nucleotide exchange factors (GEFs) on the RAB GTPases RAB1 and RAB11 ( Jones et al., 2000 ; Morozova et al...

... Ltd 2020 http://www.biologists.com/user-licence-1-1/ Highlighted Article: F-actin nucleation with Arp2/3 is triggered locally at each fusing insulin granule and controls granule dynamics and insulin secretion. Actin β cells Exocytosis Insulin Regulated secretion...

...Prasad V. Phatarpekar; Daniel D. Billadeau; Ana-Maria Lennon-Duménil ABSTRACT Natural killer (NK) cells, cytolytic lymphocytes of the innate immune system, play a crucial role in the immune response against infection and cancer. NK cells kill target cells through exocytosis of lytic granules...

... and class II formins localize to areas of active membrane turnover ( Fig. 1 , van Gisbergen et al., 2012 , 2018 ; Vidali et al., 2009b ), where both exocytosis and endocytosis occur. To investigate whether formin localization correlates with endocytic activity, we stained live cells with FM4-64...

... was statistically indistinguishable from that of Wt IHCs ( Fig. 5 A). Furthermore, we performed measurements of membrane capacitance increments (ΔC m ), which reports exocytosis evoked by the Ca 2+ influx. SV pool dynamics were probed by a train of stimuli that consisted of ten step depolarizations to −14 mV of 20...

... Ltd 2019 Summary: Why is membrane trafficking rate-limiting for release of constitutively secreted interleukin-6? We propose that cells monitor and adjust cytokine secretion rates for prevention of septic shock. Cytokine secretion Exocytosis Interleukin 6 Membrane trafficking...

... granule exocytosis. Ca 2+ , in turn, is also rapidly taken up by the mitochondria and exerts important feedback regulation of metabolism. The aim of this study was to determine whether the distribution of mitochondria within beta cells is important for the secretory capacity of these cells. We find...

...Jeffrey S. Dason; Aaron M. Allen; Oscar E. Vasquez; Marla B. Sokolowski ABSTRACT Sustained neurotransmission requires the tight coupling of synaptic vesicle (SV) exocytosis and endocytosis. The mechanisms underlying this coupling are poorly understood. We tested the hypothesis that a cGMP-dependent...

...Camille Lenzi; Jennifer Stevens; Daniel Osborn; Matthew J. Hannah; Ruben Bierings; Tom Carter ABSTRACT Elevations of intracellular free Ca 2+ concentration ([Ca 2+ ] i ) are a potent trigger for Weibel–Palade body (WPB) exocytosis and secretion of von Willebrand factor (VWF) from endothelial cells...

... in secretion of a STAT-activating cytokine. Live imaging suggests that changes in intracellular Ca 2+ are linked to this event. Our data suggest a cell-type specific requirement for particular vesicle trafficking components in regulated exocytosis during development. Given the central role for STAT signaling...

...Chien-Chang Huang; Tai-Yu Chiu; Tzu-Ying Lee; Hsin-Jui Hsieh; Chung-Chih Lin; Lung-Sen Kao ABSTRACT α-Synuclein is associated with Parkinson's disease, and is mainly localized in presynaptic terminals and regulates exocytosis, but its physiological roles remain controversial. Here, we studied...

...://www.biologists.com/user-licence-1-1/ Highlighted Article: Cholera toxin inhibits the exocytosis function of the SNX27-retromer complex, thereby inhibiting cargo trafficking to the PM. SNX27 Retromer NHE3 PDZ Secretory diarrhea Apical trafficking Exocytosis Cholera toxin Early endosomes...

... is von Willebrand factor (VWF) which, following stimulation with secretagogues, is released into the blood vessel lumen as large platelet-catching strings. This exocytosis changes the protein composition of the cell surface and also results in a net increase in the amount of plasma membrane. Compensatory...

...-licence-1-1/ Highlighted Article: Kiss-and-run and cavicapture are the dominant mechanisms associated with granule replenishment in mast cells after IgE-mediated degranulation. Kiss-and-run Cavicapture Exocytosis Endocytosis Proteoglycan Mast cell Mast cells are long-lived...

... targeting of Akt to a region of the cell marked the sites where IRAP–pHluorin vesicles fused, supporting the idea that local Akt-mediated signaling regulates exocytosis. Taken together, these results indicate that PI3K and Akt play distinct roles, and that PI3K stimulates Akt-independent pathways...

...Nadine Kittelberger; Markus Breunig; René Martin; Hans-Joachim Knölker; Pika Miklavc ABSTRACT Actin and actin-associated proteins have a pivotal effect on regulated exocytosis in secretory cells and influence pre-fusion as well as post-fusion stages of exocytosis. Actin polymerization on secretory...