..., to tether microtubules to the AJs. Here, we show that knockout (KO) of CGNL1, but not of PLEKHA7, results in the loss of junctional CAMSAP3 and its redistribution into a cytoplasmic pool both in cultured epithelial cells in vitro and mouse intestinal epithelium in vivo . In agreement, GST pulldown analyses...

... at a Glance article and the accompanying poster. The apical plasma membrane of many types of epithelial cells is enriched in cholesterol and glycosphingolipids, such as galactosylceramide sulfate (also known as sulfatide) and Forssman glycolipid ( Hansson et al., 1986 ; Koichi et al., 1974 ; Nichols et al...

...Amber J. Park; Madison A. Wright; Elyse J. Roach; Cezar M. Khursigara ABSTRACT The age-old saying, seeing is believing, could not be truer when we think about the value of imaging interactions between epithelial cells and bacterial pathogens. Imaging and culturing techniques have vastly improved...

... correlation, 0.73) ( Fig. S5E ). As human mammary epithelial cells were used in the previous study, the differing results might be due to the difference in cell type. Stretch stimuli enhances the cellular contractile force through actin filaments and myosin II (actomyosin) ( Mizutani et al., 2009...

...Brian Belardi; Sungmin Son; Michael D. Vahey; Jinzhi Wang; Jianghui Hou; Daniel A. Fletcher ABSTRACT Tight junctions have been hypothesized to act as molecular fences in the plasma membrane of epithelial cells, helping to form differentiated apical and basolateral domains. While this fence function...

.... Moreover, a stiff extracellular matrix dampens the oscillations of the epithelial molecular clock. Here, we extend this analysis to other tissues and cell types, and identify an inverse relationship between circadian clocks in epithelia and fibroblasts. Epithelial cells from mammary gland, lung and skin...

.... Cell polarity Epithelial Cells Tight junctions Morphogenesis Lumen Formation The ability to form and expand lumens is an essential characteristic of epithelia within tubular organs, such as kidneys and intestines, and is indispensable to their function. Such lumens are lined by a monolayer...

... cells, microtubules form parallel arrays aligned along the apico–basal axis, and recent work has demonstrated that the members of CAMSAP/Patronin family control apical tethering of microtubule minus ends. Here, we show that in mammalian intestinal epithelial cells, the spectraplakin ACF7 (also known...

.../ Summary: Translation affects adhesion and invasion potential of spreading initiation centers (SICs) producing mesenchymal cells, but not epithelial cells, which are deprived of SICs Spreading initiation centers SICs RNA-binding proteins Focal adhesion Translation regulation Invasion...

... Epithelial cells Smooth septate junction Tetraspanin Epithelia separate distinct fluid compartments within the bodies of metazoans. For this epithelial function, specialized intercellular junctions, designated as occluding junctions, regulate the free diffusion of solutes through the paracellular...

...Hideki Yamamoto; Chihiro Awada; Shinji Matsumoto; Tomoyuki Kaneiwa; Takayuki Sugimoto; Toshifumi Takao; Akira Kikuchi ABSTRACT Wnt5a regulates planar cell polarity in epithelial cells, but it remains to be determined whether Wnt5a and its receptors are sorted apically or basolaterally, and how...

... and Schweisguth, 2003 ), promotes the basal-to-apical transcytosis of Delta in epithelial cells ( Benhra et al., 2010 ). Furthermore, the new adherens junctions at the apical side have also been proposed to act as a platform for Notch signalling during cytokinesis because Notch accumulates there in SOPs that have...

... collagen matrices; however, its regulation during tubulogenesis is not understood. Here, we report that degradation of collagen in polarized epithelial cells is post-translationally regulated by changing the localization of MT1-MMP from the apical to the basal surface. MT1-MMP predominantly localizes...

...), that crosslinks MTs through its N-terminal MT-binding region and subsequent coiled-coil motifs. MTCL1 colocalized with the apicobasal MT bundles in epithelial cells, and its knockdown impaired the development of these MT bundles and the epithelial-cell-specific columnar shape. Rescue experiments revealed...

... at the apex of the lateral membrane in polarized MDCK cells, involves myosin IIA and might be important for the regulation of cortical actin dynamics. * Author for correspondence ( laurence.mery@u-psud.fr ) 25 1 2010 © 2010. 2010 Calcium signaling Epithelial cells Inositol (1,4,5...

... of an N-terminal KIND domain, a FERM domain and three PDZ domains. Here we show that FRMPD2 is localized in a polarized fashion in epithelial cells at the basolateral membrane and partially colocalizes with the tight-junction marker protein Zonula-occludens-1. Downregulation of FRMPD2 protein in Caco-2...

... and -independent steps in the establishment of epithelial cell polarity in Drosophila. J. Cell Biol. 167 , 135 -147. Harris, T. J. and Peifer, M. ( 2005 ). The positioning and segregation of apical cues during epithelial polarity establishment in Drosophila. J. Cell Biol. 170 , 813 -823. Hirose, T...

... lineages that show differing sensitivities to anoikis. We demonstrate that both fibroblasts and epithelial cells prevent anoikis through FAK activation. We show that FAK activates multiple downstream pathways in order to suppress anoikis. However FAK regulates survival through a more restricted set...

...Masaru Kishikawa; Atsushi Suzuki; Shigeo Ohno Atypical protein kinase C (aPKC) generally plays crucial roles in the establishment of cell polarity in various biological contexts. In mammalian epithelial cells, aPKC essentially works towards the transition of primordial spot-like adherens junctions...

... with transmembrane TGFα and decreases its ectodomain shedding to release soluble TGFα. Here we report that CD9 has a role in the maturation of transmembrane TGFα and its stabilization at the cell surface, and in the cell-surface distribution in polarized epithelial cells. Furthermore, coexpression of CD9 and TGFα...