... transport in the endosomal system as it has been shown to mediate ‘kiss-and-run’ vesicle–target membrane interactions. In this Cell Science at a Glance and the accompanying poster, we compare the structure of the coiled-coil and the multisubunit CATCHR and class C Vps tether families on the basis...

... of the cell requires endosomes and lysosomes to be proficient in decision-making: fusion or fission; recycling or degradation; fast transport or contacts with other organelles. To effectively discriminate between these options, the endolysosomal system employs complex regulatory strategies that crucially rely...

...Marlieke L. M. Jongsma; Nina Bakker; Jacques Neefjes ABSTRACT The endosomal system orchestrates the transport of lipids, proteins and nutrients across the entire cell. Along their journey, endosomes mature, change shape via fusion and fission, and communicate with other organelles. This intriguing...

... are endocytosed and undergo retrograde trafficking to the soma, where downstream signalling occurs. Vesicles transporting neurotrophic receptors to the soma are reported to be Rab7-positive late endosomes and/or multivesicular bodies (MVBs), where receptors localize within so-called intraluminal vesicles (herein...

...Zachary N. Wilson; Dalton Buysse; Matt West; Daniel Ahrens; Greg Odorizzi ABSTRACT Endosomes undergo a maturation process highlighted by a reduction in lumenal pH, a conversion of surface markers that prime endosome–lysosome fusion and the sequestration of ubiquitylated transmembrane protein cargos...

... phosphatidylinositol 3-phosphate (PtdIns3P) on their limiting membrane as they mature into endosomal-like vesicles. The molecular mechanisms that underlie recycling of membranes and transmembrane proteins from these macropinosomes still need to be defined. Here, we report that JIP4 (officially known as SPAG9...

... this miRNA. We found that EV-associated miR-122 is primarily single-stranded and, to become functional, is loaded onto the recipient cell argonaute proteins without requiring host Dicer1. Following endocytosis, EV-associated miR-122 is loaded onto the host cell argonaute proteins on the endosomal membrane...

.... Summary: A heterodimeric SNX4–SNX7 SNX-BAR complex regulates mammalian autophagosome assembly through the control of ATG9 trafficking. Sorting nexin SNX4 SNX7 SNX30 Endosomes Autophagy ATG9A Macroautophagy (herein referred to as autophagy) describes the sequestration of cytoplasmic...

... and motor function, epileptic seizures and a significantly reduced lifespan. CLN3 localizes to endosomes and lysosomes, and has been implicated in intracellular trafficking and autophagy. However, the precise molecular function of CLN3 remains to be elucidated. Previous studies showed an interaction between...

...-affinity antibody responses, B cells internalise specific antigens and process them into peptides loaded onto MHCII for presentation to T helper cells (T H cells). While the biochemical principles of antigen processing and MHCII loading have been well dissected, how the endosomal vesicle system is wired...

... late endosomes to the trans-Golgi. An siRNA screen targeting proteins containing TBC domains critical for Rab GAPs was performed and coupled to a phenotypic read-out that visualized the distribution of Rab7b. Silencing of TBC1D5 provided the strongest phenotype and this protein was subsequently...

...Jacqueline Staring; Matthijs Raaben; Thijn R. Brummelkamp ABSTRACT In order to replicate, most pathogens need to enter their target cells. Many viruses enter the host cell through an endocytic pathway and hijack endosomes for their journey towards sites of replication. For delivery of their genome...

... as regulators of cell migration. The adaptor protein containing a pleckstrin-homology (PH) domain, phosphotyrosine binding (PTB) domain, and leucine zipper motif 1 (APPL1) is a 709 amino acid endosomal protein that plays a role in cell proliferation and survival as well as endosomal trafficking and signaling...

... processes. In order to control these signals, activated receptors are endocytosed and thoroughly curated by the endosomal network of intracellular vesicles and proteolytic organelles. In this Review, we follow the epidermal growth factor (EGF) receptor (EGFR) from ligand engagement, through its voyage...

...Graziana Modica; Olga Skorobogata; Etienne Sauvageau; Adriano Vissa; Christopher M. Yip; Peter K. Kim; Hugo Wurtele; Stephane Lefrancois ABSTRACT Retromer is a multimeric protein complex that mediates endosome-to-trans-Golgi network (TGN) and endosome-to-plasma membrane trafficking of integral...

... assays, knockout of CAMSAP3, but not of CAMSAP2, caused microtubule re-organization into a more radial centrosomal array, redistribution of Rab11-positive (also known as Rab11A) endosomes from the apical cell surface to the pericentrosomal region and inhibition of actin brush border formation...

...Tatsuyuki Matsudaira; Takahiro Niki; Tomohiko Taguchi; Hiroyuki Arai ABSTRACT The retrograde pathway is defined by the transport of proteins and lipids from the plasma membrane through endosomes to the Golgi complex, and is essential for a variety of cellular activities. Recycling endosomes...

...Marita Borg; Oddmund Bakke; Cinzia Progida ABSTRACT Rab proteins are small GTPases that regulate transport between the different compartments of the endomembrane system in eukaryotic cells. Here, we show that Rab7b, a Rab that controls the transport between late endosomes and the trans Golgi...

... Brickner   J. H. , Fuller   R. S. ( 1997 ). SOI1 encodes a novel, conserved protein that promotes TGN-endosomal cycling of Kex2p and other membrane proteins by modulating the function of two TGN localization signals.   J. Cell Biol.   139 , 23 – 36 . 10.1083/jcb.139.1.23 Bryant   N. J...

...Satoshi Kametaka; Ai Kametaka; Shinichi Yonekura; Mineyuki Haruta; Seiichi Takenoshita; Satoshi Goto; Satoshi Waguri Clathrin adaptor protein complex-1 (AP-1) and its accessory proteins play a role in the sorting of integral membrane proteins at the trans -Golgi network and endosomes...