... that depletion of active eIF5A in mouse fibroblasts reduced collagen type I α1 chain (Col1a1) content, which concentrated around the nuclei. Moreover, it provoked the upregulation of endoplasmic reticulum (ER) stress markers, suggesting retention of partially synthesized collagen 1 (Col1) in the ER. We confirmed...

... , C. M. and Urano , F. ( 2011 ). Measuring ER stress and the unfolded protein response using mammalian tissue culture system . Methods Enzymol. 490 , 71 - 92 . 10.1016/B978-0-12-385114-7.00004-0 Pagano , A. , Letourneur , F. , Garcia-Estefania , D. , Carpentier , J...

...Andria A. Lytridou; Anthi Demetriadou; Melina Christou; Louiza Potamiti; Nikolas P. Mastroyiannopoulos; Kyriacos Kyriacou; Leonidas A. Phylactou; Anthi Drousiotou; Petros P. Petrou ABSTRACT Imbalances in endoplasmic reticulum (ER) homeostasis provoke a condition known as ER stress and activate...

...Hery Urra; Philippe Pihán; Claudio Hetz ABSTRACT Different perturbations alter the function of the endoplasmic reticulum (ER), resulting in the accumulation of misfolded proteins in its lumen, a condition termed ER stress. To restore ER proteostasis, a highly conserved pathway is engaged, known...

...Imadeddin Hijazi; Jeffrey Knupp; Amy Chang ABSTRACT One major cause of endoplasmic reticulum (ER) stress is homeostatic imbalance between biosynthetic protein folding and protein folding capacity. Cells utilize mechanisms such as the unfolded protein response (UPR) to cope with ER stress...

... induces endoplasmic reticulum (ER) stress in primary macrophages and in murine macrophage cell line Raw 264.7, in which the unfolded protein response is initiated to modulate cytokine production, thereby activating the immune response. Stressors that initiate the ER stress response block store-dependent...

...) in cells expressing p.S143P lamin A/C. Additional endoplasmic reticulum (ER) stress induced by tunicamycin reduced the viability of cells expressing mutant lamin further. In summary, p.S143P lamin A/C affects normal lamina structure and influences the cellular stress response, homeostasis and viability...

...Vittoria Pagliarini; Paola Giglio; Paolo Bernardoni; Daniela De Zio; Gian Maria Fimia; Mauro Piacentini; Marco Corazzari ABSTRACT The endoplasmic reticulum (ER) has recently emerged as an alternative target to induce cell death in tumours, because prolonged ER stress results in the induction...

...Modi Safra; Shani Ben-Hamo; Cynthia Kenyon; Sivan Henis-Korenblit Summary The unfolded protein response (UPR) allows cells to cope with endoplasmic reticulum (ER) stress by adjusting the capacity of the ER to the load of ER-associated tasks. The UPR is important for maintaining ER homeostasis under...

... organ tissue. The molecular mechanisms leading to renal fibrosis are not fully understood. Here we demonstrated that TGFβ1, AGT or PDGF stimulation of renal cells resulted in endoplasmic reticulum (ER) stress followed by activation of the protective unfolded protein response pathway and a high secretory...

... to ER stress-inducing compounds, but not other cell stressors that we tested in insulinoma cell lines. SDF2L1 protein levels were also induced by expression of misfolded proinsulin in insulinoma cells and in islets from diabetic mice. Immunoprecipitation and binding assays demonstrated that SDF2L1...

... keratinization (dyskeratosis) resulting in characteristic mucocutaneous abnormalities. However, the molecular pathogenic mechanism by which these changes influence keratinocyte adhesion and viability remains unknown. We show here that SERCA2 protein is extremely sensitive to endoplasmic reticulum (ER) stress...

...Kwok Yeung Tsang; Danny Chan; John F. Bateman; Kathryn S. E. Cheah Disturbances to the balance of protein synthesis, folding and secretion in the endoplasmic reticulum (ER) induce stress and thereby the ER stress signaling (ERSS) response, which alleviates this stress. In this Commentary, we review...

... are, however, efficiently killed by thapsigargin, a specific inhibitor of ER Ca 2+ pumps that induces ER stress by depleting ER Ca 2+ stores. In the presence of Bak and Bax, thapsigargin eliminates cells by release of mitochondrial cytochrome c and subsequent caspase activation, which leads to the proteolytic...

... ( lisa.swanton@manchester.ac.uk ) 1 9 2009 © The Company of Biologists Limited 2009 2009 ER-associated degradation ER stress Pelizaeus-Merzbacher disease Protein misfolding Unfolded protein response A number of clinically important human diseases are known to be caused by protein...

... in the cytoplasm. * Author for correspondence ( hide@biophysics.mbox.media.kyoto-u.ac.jp ) 2 6 2009 © The Company of Biologists Limited 2009 2009 ER stress Unfolded protein response RNA splicing IRE1 XBP1 The endoplasmic reticulum (ER) is an organelle in which both...

... and lysosomal compartments and subjected to ER-stress-associated protein aggregation. D2SV aggregation relies on the motor activities of dynein and is blocked by ER stress modulators. The ability of D2SV to sequester other cell cycle proteins provides a mechanistic explanation for its effects on cardiomyocyte...

... expressed protein is recruited to stress granules induced in cell lines, oligodendrocytes and neurons by several stress stimuli, including oxidative stress, ER stress or heat shock ( Thomas et al., 2005 ). Given that Stau1 binds to mRNAs and ribosomal subunits ( Kiebler et al., 1999 ; Marion et al., 1999...

...Luca Ulianich; Corrado Garbi; Antonella Sonia Treglia; Dario Punzi; Claudia Miele; Gregory Alexander Raciti; Francesco Beguinot; Eduardo Consiglio; Bruno Di Jeso Conditions perturbing the homeostasis of the endoplasmic reticulum (ER) cause accumulation of unfolded proteins and trigger ER stress...

...Hiroshi Shiraishi; Hideaki Okamoto; Akihiko Yoshimura; Hiroki Yoshida Accumulation of unfolded proteins induces endoplasmic reticulum (ER) stress. Excessive and prolonged stresses lead cells to apoptosis. However, the precise molecular mechanisms of ER stress-induced apoptosis have not been fully...