... for formation of Dvl2 condensates during Wnt signalling. Ubiquitin Phase separation Dishevelled WWP2 Protein condensates Wnt signalling Department of Science & Technology, Government of India https://doi.org/10.13039/501100001409 EMR/2016/006928 Department of Biotechnology...

...Juliusz Mieszczanek; Helen Strutt; Trevor J. Rutherford; David Strutt; Mariann Bienz; Melissa V. Gammons ABSTRACT Dishevelled is a cytoplasmic hub that transduces Wnt signals to cytoplasmic effectors, which can be broadly characterised as canonical (β-catenin dependent) and noncanonical, to specify...

... of the Dishevelled scaffolding protein family can bind the lipid kinases phosphatidylinositol 4-kinase (PI4K) and phosphatidylinositol 4-phosphate 5-kinase (PIP5K), facilitating synthesis of PtdIns P 2 directly from phosphatidylinositol. We used several assays for PtdIns P 2 to examine the cooperative function...

...Melissa V. Gammons; Trevor J. Rutherford; Zachary Steinhart; Stephane Angers; Mariann Bienz ABSTRACT Dishevelled (DVL) assembles Wnt signalosomes through dynamic head-to-tail polymerisation by means of its DIX domain. It thus transduces Wnt signals to cytoplasmic effectors including β-catenin...

...Austin T. Baldwin; Amy M. Clemons; Bryan T. Phillips ABSTRACT The Wnt/β-catenin signaling pathway is utilized across metazoans. However, the mechanism of signal transduction, especially dissociation of the β-catenin destruction complex by Dishevelled proteins, remains controversial. Here, we...

...Rama Kamesh Bikkavilli; Craig C. Malbon Wnt signaling is initiated upon binding of Wnt proteins to Frizzled proteins and their co-receptors LRP5 and 6. The signal is then propagated to several downstream effectors, mediated by the phosphoprotein scaffold, dishevelled. We report a novel role...

...Rama Kamesh Bikkavilli; Craig C. Malbon Wnt/β-catenin signaling is essential for normal mammalian development. Wnt3a activates the Wnt/β-catenin pathway through stabilization of β-catenin; a process in which the phosphoprotein Dishevelled figures prominently. Protein arginine methylation...

...Noriko Yokoyama; Urszula Golebiewska; Hsien-yu Wang; Craig C. Malbon Dishevelled-3 (Dvl3) is a multivalent scaffold protein that is essential to Wnt signaling during development. Although Dvl-based punctae have been visualized by fluorescence microscopy; the physical nature and dynamic character...

...Ciara Metcalfe; Carolina Mendoza-Topaz; Juliusz Mieszczanek; Mariann Bienz Wnt/β-catenin signalling controls cell fates in development, tissue homeostasis and cancer. Wnt binding to Frizzled receptors triggers recruitment of Dishevelled to the plasma membrane and formation of a signalosome...

... and primitive endoderm formation in response to Wnt3a, implicating Src as a positive regulator of Wnt/β-catenin signaling. We discovered that Src binds dishevelled-2 (Dvl2), a key phosphoprotein in Wnt signaling, at two positions: an SH3-binding domain and a C-terminal domain. The Y18F mutant of Dvl2 attenuates...

.... Furthermore, epistasis experiments pinpoint p38 MAPK as operating downstream of Dishevelleds. We also demonstrate that chemical inhibition of p38 MAPK restores Wnt3a-attenuated GSK3β kinase activity. We demonstrate the involvement of G-proteins and Dishevelleds in Wnt3a-induced p38 MAPK activation...

...Rama Kamesh Bikkavilli; Michael E. Feigin; Craig C. Malbon In Drosophila , activation of Jun N-terminal Kinase (JNK) mediated by Frizzled and Dishevelled leads to signaling linked to planar cell polarity. A biochemical delineation of WNT-JNK planar cell polarity was sought in mammalian cells...

...Thomas Schwarz-Romond; Ciara Metcalfe; Mariann Bienz Dishevelled (Dvl) proteins are cytoplasmic components of the Wnt signalling pathway, which controls numerous cell fate decisions during animal development. During Wnt signalling, Dvl binds to the intracellular domain of the frizzled transmembrane...

...Vítězslav Bryja; Gunnar Schulte; Nina Rawal; Alexandra Grahn; Ernest Arenas Previously, we have shown that Wnt-5a strongly regulates dopaminergic neuron differentiation by inducing phosphorylation of Dishevelled (Dvl). Here, we identify additional components of the Wnt-5a-Dvl pathway...

.... The Fz-LRP receptor complex probably accomplishes this by generating multiple signals in the cytoplasm. These involve activation of Dishevelled (Dsh), possibly through trimeric G proteins and LRP-mediated axin binding and/or degradation. However, individual Wnts and Fzs can activate both β-catenin...

...Thomas Schwarz-Romond; Christien Merrifield; Benjamin J. Nichols; Mariann Bienz Dishevelled is a crucial effector upstream in the Wnt signalling pathway, but the molecular mechanism by which it transduces the Wnt signal remains elusive. Dishevelled is a cytoplasmic protein with a strong tendency...

...Matthew J. Smalley; Nathalie Signoret; David Robertson; Alan Tilley; Anthony Hann; Ken Ewan; Yanning Ding; Hugh Paterson; Trevor C. Dale Dishevelled family proteins are multidomain intracellular transducers of Wnt signals. Ectopically expressed mammalian Dishevelled 2 (Dvl-2) activates downstream...

...) and Dishevelled (Dsh) function as key players in PCP signalling. Although Fz and Dsh are also involved in Wingless (Wg)/Wnt signalling, these proteins have independent functions in a non-canonical pathway dedicated to PCP. Reorganization of the cell surface and cytoskeleton is required, and recent work has...

... as convergent extension. During these Pax3-induced processes, Dishevelled and Frizzled are localized to the actin cytoskeleton and both proteins coimmunoprecipitate focal adhesion components from detergent-insoluble cell fractions. We show further that these Pax3-induced cell movements are associated...