... at the centrosome with a concomitant increase in centrosomal proteins PCM1 and CEP131, which was rescued by exogenous ATG4B. The two-stage cell cycle defects following ATG4B and ATG4A loss have important therapeutic implications for PDAC. * Author for correspondence: sgorski@bcgsc.ca 23 9 2022 17...

...Priti S. Atmakuru; Jyotsna Dhawan ABSTRACT The centrosome is an evolutionarily conserved, ancient organelle whose role in cell division was first described over a century ago. The structure and function of the centrosome as a microtubule-organizing center, and of its extracellular extension...

... and mitosis, calcineurin consistently associated with multiple centrosomal and/or ciliary proteins. These include POC5, which binds centrins in a Ca 2+ -dependent manner and is a component of the luminal scaffold that stabilizes centrioles. We show that POC5 contains a calcineurin substrate motif (PxIxIT type...

...Dandan Ma; Fulin Wang; Junlin Teng; Ning Huang; Jianguo Chen ABSTRACT Centrosomes are composed of centrioles surrounded by pericentriolar material. The two centrioles in G1 phase are distinguished by the localization of their appendages in the distal and subdistal regions; the centriole possessing...

... are restricted to proliferating crypt cells, including Lgr5+ stem cells, where the Mcc protein is distinctly associated with the centrosome. Upon intestinal cellular differentiation, Mcc is redeployed to the apical domain of polarized villus cells where non-centrosomal microtubule organizing centers (ncMTOCs...

... in ciliary cell biology. Basal body Centrosome Cilia Cytoplasmic transport Diffusion Motor proteins National Heart, Lung, and Blood Institute https://doi.org/10.13039/100000050 R01HL117164 Eunice Kennedy Shriver National Institute of Child Health and Human Development https...

... of the centriole cartwheel at the proximal end. CP110 Cep97 Polo-like kinase 4 Centriole Centrosome Organelle biogenesis Cell cycle Embryogenesis Wellcome Trust http://dx.doi.org/10.13039/100010269 104575 215523 Edward Penley Abraham Scholarship Sandler Foundation...

... of Thr3 phosphorylation on histone H3 (H3T3ph) does not alter borealin and SGO2 centromeric localization. Meiosis Centromere Centrosome Haspin H3T3ph Aurora B Ministerio de Economía y Competitividad http://dx.doi.org/10.13039/501100003329 Ministerio de Ciencia e Innovación...

... to the centrosome/basal body independently of EHD1, retromer acts primarily at an early stage of ciliogenesis similar to EHD1 and MICAL-L1. Removal of the CP110 capping protein from the mother centriole is a critical step in ciliogenesis, and Rab11, EHD1 and MICAL-L1 are required to facilitate this event ( Feng et...

...Morgan Le Roux-Bourdieu; Devashish Dwivedi; Daniela Harry; Patrick Meraldi ABSTRACT Centrioles are central structural elements of centrosomes and cilia. In human cells, daughter centrioles are assembled adjacent to existing centrioles in S-phase and reach their full functionality with the formation...

... proposed that the APC/C might fulfil other functions, including assembly of the mitotic spindle. Here, we show that the APC/C localizes to centrosomes, the organizers of the eukaryotic microtubule cytoskeleton, specifically during mitosis. Recruitment of the APC/C to spindle poles requires the centrosomal...

... in ciliary protein trafficking, in the assembly of the T cell synapse. We demonstrated that BBS1 allows for centrosome polarization towards the immune synapse. This function is achieved through the clearance of centrosomal F-actin and its positive regulator WASH1 (also known as WASHC1), a process that we...

... and reproduction in any medium provided that the original work is properly attributed. Centrioles are barrel-shaped microtubule-based structures that form the core of centrosomes. Centrioles have multiple different appendages (see Box 1 for an account of mammalian centrosome components and associated...

...Jeffrey C. Medley; Joseph R. DiPanni; Luke Schira; Blake M. Shaffou; Brandon M. Sebou; Mi Hye Song ABSTRACT Aberrant centrosome numbers are associated with human cancers. The levels of centrosome regulators positively correlate with centrosome number. Thus, tight control of centrosome protein...

... multiple functions at centrioles and centrosomes, and we have previously shown that in Drosophila phosphorylated Sas-4 initiates Polo recruitment to newly formed centrioles, while phosphorylated Spd-2 recruits Polo to the pericentriolar material (PCM) that assembles around mother centrioles in mitosis...

...Nina Schweizer; Jens Lüders ABSTRACT Centrioles are microtubule-based cylindrical structures that assemble the centrosome and template the formation of cilia. The proximal part of centrioles is associated with the pericentriolar material, a protein scaffold from which microtubules are nucleated...

... defects. However, the regulation of cytoplasmic PLK1 is not fully understood. In this study, we show that CEP76, a centriolar protein, physically interacts with PLK1 and tightly controls the activation of cytoplasmic PLK1 during mitosis in human cells. We found that removal of centrosomes induced ectopic...

...Yuki Yoshino; Akihiro Kobayashi; Huicheng Qi; Shino Endo; Zhenzhou Fang; Kazuha Shindo; Ryo Kanazawa; Natsuko Chiba ABSTRACT Breast cancer gene 1 (BRCA1) contributes to the regulation of centrosome number. We previously identified receptor for activated C kinase 1 (RACK1) as a BRCA1-interacting...

... is very sensitive to defective centrioles. Centrioles are microtubule-based organelles that recruit PCM to form the centrosome, which is the main MTOC in animal cells. The centrosome is essential for accurate chromosome segregation during mitosis, which is critical for the maintenance of genomic...

... assembly proteins [Plk4, Ana2 (the homologue of mammalian STIL), Sas-6, Sas-4 (mammalian CPAP) or Asl (mammalian Cep152)] leads to the absence of centrioles. Here, we use Sas-6 and Ana2 particles (SAPs) as a new model to probe the pathway of centriole and centrosome assembly. SAPs form in Drosophila eggs...