...Mustafa G. Aydogan; Laura E. Hankins; Thomas L. Steinacker; Mohammad Mofatteh; Saroj Saurya; Alan Wainman; Siu-Shing Wong; Xin Lu; Felix Y. Zhou; Jordan W. Raff ABSTRACT Centrioles are composed of a central cartwheel tethered to nine-fold symmetric microtubule (MT) blades. The centriole cartwheel...

... clearance of airways in mammals. These epithelia are composed of multiciliated cells (MCCs) harboring myriads of motile cilia, which rest on modified centrioles called basal bodies (BBs), and beat coordinately to generate directed fluid flows. Thus, BB biogenesis and organization is central to MCC function...

... multiple functions at centrioles and centrosomes, and we have previously shown that in Drosophila phosphorylated Sas-4 initiates Polo recruitment to newly formed centrioles, while phosphorylated Spd-2 recruits Polo to the pericentriolar material (PCM) that assembles around mother centrioles in mitosis...

...Nina Schweizer; Jens Lüders ABSTRACT Centrioles are microtubule-based cylindrical structures that assemble the centrosome and template the formation of cilia. The proximal part of centrioles is associated with the pericentriolar material, a protein scaffold from which microtubules are nucleated...

...Andrew P. Porter; Hannah Reed; Gavin R. M. White; Erinn-Lee Ogg; Helen J. Whalley; Angeliki Malliri ABSTRACT Centriole duplication is tightly controlled to maintain correct centriole number through the cell cycle. Key to this is the regulated degradation of PLK4, the master regulator of centriole...

... or financial interests. 4 11 2019 24 8 2020 © 2020. Published by The Company of Biologists Ltd 2020 http://www.biologists.com/user-licence-1-1/ Summary: The centriole protein CEP76 is essential for the proper regulation of PLK1 in the cytoplasm to prevent its aberrant...

...Huijie Zhao; Sen Yang; Qingxia Chen; Xiaomeng Duan; Guoqing Li; Qiongping Huang; Xueliang Zhu; Xiumin Yan ABSTRACT The Cep63–Cep152 complex located at the mother centriole recruits Plk4 to initiate centriole biogenesis. How the complex is targeted to mother centrioles, however, is unclear...

...Yi-Nan Lin; Ying-Shan Lee; Shu-Kuei Li; Tang K. Tang ABSTRACT Primary microcephaly (MCPH) is a neurodevelopmental disorder characterized by small brain size with mental retardation. CPAP (also known as CENPJ ), a known microcephaly-associated gene, plays a key role in centriole biogenesis. Here, we...

...Lisa Gartenmann; Catarina C. Vicente; Alan Wainman; Zsofi A. Novak; Boris Sieber; Jennifer H. Richens; Jordan W. Raff ABSTRACT Centriole assembly requires a small number of conserved proteins. The precise pathway of centriole assembly has been difficult to study, as the lack of any one of the core...

... associate with BBs and function in BB assembly, maturation and stability. Human POC5 (hPOC5) is a highly conserved centrin-binding protein that binds centrins through Sfi1p-like repeats and is required for building full-length, mature centrioles. Here, we use the BB-rich cytoskeleton of Tetrahymena...

... is necessary for robust PLK4 activation and centriole duplication. Here, we show that CEP85 and STIL are also required for directional cancer cell migration. Mutational and functional analyses reveal that the interactions between CEP85, STIL and PLK4 are essential for effective directional cell motility...

...Shuwei Xie; Trey Farmer; Naava Naslavsky; Steve Caplan ABSTRACT The endocytic protein EHD1 plays an important role in ciliogenesis by facilitating fusion of the ciliary vesicle and removal of CP110 (also known as CCP110) from the mother centriole, as well as removal of Cep215 (also known...

... extent as deletion of centrin 2. Phenotypic analysis of the 4DA mutant revealed defects in centrosome localization, centriole satellite assembly, ciliary assembly and function and in interactions with POC5 and SFI1. These observations indicate that centrin 2 requires calcium-binding capacity for its...

... centriole. SAS-6 proteins are thought to constitute the hub of the cartwheel structure. However, the exact function of the cartwheel in the process of centriole formation has not been well characterized. In this study, we focused on the functions of human SAS-6 (HsSAS-6, also known as SASS6). By using...

...Pierre Gönczy; Georgios N. Hatzopoulos ABSTRACT The centriole organelle consists of microtubules (MTs) that exhibit a striking 9-fold radial symmetry. Centrioles play fundamental roles across eukaryotes, notably in cell signaling, motility and division. In this Cell Science at a Glance article...

...Saroj Saurya; Hélio Roque; Zsofia A. Novak; Alan Wainman; Mustafa G. Aydogan; Adam Volanakis; Boris Sieber; David Miguel Susano Pinto; Jordan W. Raff ABSTRACT Centrioles organise centrosomes and cilia, and these organelles have an important role in many cell processes. In flies, the centriole...

...Marco Gottardo; Giuliano Callaini; Maria Giovanna Riparbelli ABSTRACT We report, here, that two distinct centriole lineages exist in Drosophila : somatic centrioles usually composed by microtubule doublets and germ line centrioles characterized by triplets. Remarkably, the transition from doublets...

...Lukas Čajánek; Timo Glatter; Erich A. Nigg Centrioles function as core components of centrosomes and as basal bodies for the formation of cilia and flagella. Thus, effective control of centriole numbers is essential for embryogenesis, tissue homeostasis and genome stability. In mammalian cells...

... plasticity. Although centrioles, cilia and flagella have cold-stable microtubules, MAP6 proteins have not been identified in these organelles, suggesting that additional proteins support this role in these structures. Here, we characterize human FAM154A (hereafter referred to as hSAXO1) as the first human...

... by The Company of Biologists Ltd 2014 Cell cycle Centrosome cycle Centriole Pericentriolar material Mitotic kinase Spindle assembly The centrosome is the main microtubule-organizing center (MTOC) in animal cells. Under certain circumstances, the centrosome also acts as the basal body...