.... * These authors contributed equally to this work ‡ Author for correspondence ( rachael.murray@qut.edu.au ) 28 5 2012 © 2012. Published by The Company of Biologists Ltd 2012 Flightless Late endosome Lysosome Secretion TNF Cathepsin D Rab7 Stx11 Flightless (Flii) is a member...

... to the lysosomes, perhaps because misfolding of the mutant protein masks sorting signals required for proper trafficking. The digestion of phagocytized outer segments requires a high level of lysosomal enzyme activity, and cathepsin D, the major enzyme responsible for proteolysis of the outer segments...

... Interactions between cancer cells and fibroblasts are crucial in cancer progression. We have previously shown that the aspartic protease cathepsin D (cath-D), a marker of poor prognosis in breast cancer that is overexpressed and highly secreted by breast cancer cells, triggers mouse embryonic fibroblast...

... exposure was with enhanced chemi-luminesence reagents (ECL, Amersham, UK). For the cathepsin D immunoblot, membranes were blotted with a rabbit anti-human cathepsin D (CD) antibody (4 μg/ml) followed by a HRP-conjugated anti-rabbit antibody. The positive antigen control for the cathepsin D immunoblot...

...Friederike Egberts; Michael Heinrich; Jens-Michael Jensen; Supandi Winoto-Morbach; Stephan Pfeiffer; Marc Wickel; Michael Schunck; Judith Steude; Paul Saftig; Ehrhardt Proksch; Stefan Schütze We previously demonstrated that the aspartate protease cathepsin D is activated by ceramide derived from...

... is under the control of phosphatydilinositol 3-kinase and requires the integrity of the cytoskeleton and the rise of intracellular free calcium ions. Cell death Cathepsin D Ischemia Exocytosis Signal transduction In different tissues a brief period of ischemia increases the resistance...