... but contributes to the coherent chromatin motion across the entire nucleus. However, the regulation of dynamics, nuclear orientation, and compaction of sub-regions along a single chromosome are not well-understood. We used CRISPR-based real-time single-particle tracking and polymer models to characterize...

... of cytokinesis and highlight recent studies that successfully used CRISPR-Cas (clustered regularly interspaced short palindromic repeats–CRISPR-associated proteins) technology to answer critical questions regarding the function of cytokinesis proteins. We also present methodologies for editing essential genes...

... sequence-targeting CRISPR (cr)RNAs of 23 nt spacers are used, >70% of the founders obtained are edited. Moreover, the use of Mb3Cas12a tagged to monomeric streptavidin (mSA) in conjunction with biotinylated DNA donor template leads to high knock-in efficiency in two-cell mouse embryos, with 40...