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Keywords: CRISPR
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Journal Articles
J Cell Sci (2023) 136 (8): jcs260754.
Published: 19 April 2023
...Tsz Kwan Yeung; Sehong Kim; Hoi Tang Ma; Randy Y. C. Poon ABSTRACT Switching genes on and off on cue is a cornerstone for understanding gene functions. One contemporary approach for loss-of-function studies of essential genes involves CRISPR-mediated knockout of the endogenous locus in conjunction...
Includes: Supplementary data
Journal Articles
J Cell Sci (2023) 136 (4): jcs260137.
Published: 27 February 2023
... confined but contribute to coherent chromatin motion across the entire nucleus. However, the regulation of dynamics, nuclear orientation and compaction of subregions along a single chromosome are not well-understood. We used CRISPR-based real-time single-particle tracking and polymer models to characterize...
Includes: Supplementary data
Journal Articles
J Cell Sci (2021) 134 (8): jcs254409.
Published: 22 April 2021
... of cytokinesis and highlight recent studies that successfully used CRISPR-Cas (clustered regularly interspaced short palindromic repeats–CRISPR-associated proteins) technology to answer critical questions regarding the function of cytokinesis proteins. We also present methodologies for editing essential genes...
Journal Articles
J Cell Sci (2020) 133 (9): jcs240705.
Published: 11 May 2020
... sequence-targeting CRISPR (cr)RNAs of 23 nt spacers are used, >70% of the founders obtained are edited. Moreover, the use of Mb3Cas12a tagged to monomeric streptavidin (mSA) in conjunction with biotinylated DNA donor template leads to high knock-in efficiency in two-cell mouse embryos, with 40...
Includes: Supplementary data