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J Cell Sci jcs.260137.
Published: 31 January 2023
... but contributes to the coherent chromatin motion across the entire nucleus. However, the regulation of dynamics, nuclear orientation, and compaction of sub-regions along a single chromosome are not well-understood. We used CRISPR-based real-time single-particle tracking and polymer models to characterize...
In collection:Tools in Cell Biology
J Cell Sci (2021) 134 (8): jcs254409.
Published: 22 April 2021
... of cytokinesis and highlight recent studies that successfully used CRISPR-Cas (clustered regularly interspaced short palindromic repeats–CRISPR-associated proteins) technology to answer critical questions regarding the function of cytokinesis proteins. We also present methodologies for editing essential genes...
Zhuqing Wang, Yue Wang, Shawn Wang, Andrew J. Gorzalski, Hayden McSwiggin, Tian Yu, Kimberly Castaneda-Garcia, Brian Prince, Hetan Wang, Huili Zheng, Wei Yan
J Cell Sci (2020) 133 (9): jcs240705.
Published: 11 May 2020
... sequence-targeting CRISPR (cr)RNAs of 23 nt spacers are used, >70% of the founders obtained are edited. Moreover, the use of Mb3Cas12a tagged to monomeric streptavidin (mSA) in conjunction with biotinylated DNA donor template leads to high knock-in efficiency in two-cell mouse embryos, with 40...
Includes: Supplementary data