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1-4 of 4
Keywords: CRISPR
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Journal Articles
In collection:
Tools in Cell Biology
Journal:
Journal of Cell Science
J Cell Sci (2023) 136 (8): jcs260754.
Published: 19 April 2023
...Tsz Kwan Yeung; Sehong Kim; Hoi Tang Ma; Randy Y. C. Poon ABSTRACT Switching genes on and off on cue is a cornerstone for understanding gene functions. One contemporary approach for loss-of-function studies of essential genes involves CRISPR-mediated knockout of the endogenous locus in conjunction...
Includes: Supplementary data
Journal Articles
In collection:
Exploring the Nucleus
Journal:
Journal of Cell Science
J Cell Sci (2023) 136 (4): jcs260137.
Published: 27 February 2023
... confined but contribute to coherent chromatin motion across the entire nucleus. However, the regulation of dynamics, nuclear orientation and compaction of subregions along a single chromosome are not well-understood. We used CRISPR-based real-time single-particle tracking and polymer models to characterize...
Includes: Supplementary data
Journal Articles
In collection:
Tools in Cell Biology
Journal:
Journal of Cell Science
J Cell Sci (2021) 134 (8): jcs254409.
Published: 22 April 2021
... of cytokinesis and highlight recent studies that successfully used CRISPR-Cas (clustered regularly interspaced short palindromic repeats–CRISPR-associated proteins) technology to answer critical questions regarding the function of cytokinesis proteins. We also present methodologies for editing essential genes...
Journal Articles
Zhuqing Wang, Yue Wang, Shawn Wang, Andrew J. Gorzalski, Hayden McSwiggin, Tian Yu, Kimberly Castaneda-Garcia, Brian Prince, Hetan Wang, Huili Zheng, Wei Yan
Journal:
Journal of Cell Science
J Cell Sci (2020) 133 (9): jcs240705.
Published: 11 May 2020
... sequence-targeting CRISPR (cr)RNAs of 23 nt spacers are used, >70% of the founders obtained are edited. Moreover, the use of Mb3Cas12a tagged to monomeric streptavidin (mSA) in conjunction with biotinylated DNA donor template leads to high knock-in efficiency in two-cell mouse embryos, with 40...
Includes: Supplementary data