... player, underlies the laminin-rich ECM-induced cell cycle arrest that is crucial for mammary gland development, architecture and function. Pten Basement membrane Cell cycle Extracellular matrix Mammary gland acinogenesis Quiescence Fundação de Amparo à Pesquisa do Estado de São Paulo...

... to laminin α5-containing basement membrane matrix, which is produced by luminal cells, presents such a control mechanism. Adhesion to laminin α5 directs basal cells towards a luminal cell fate, and thereby results in a marked decrease of basal MEC progenitor activity in vitro and in vivo . Mechanistically...

... of the ECM regulator, mt-mmpA , thereby modulating basement membrane thickness. In planarians, the basement membrane around the gut/inner parenchyma is thought to act as a niche for pluripotent stem cells. It has been shown that the early epidermal progenitors migrate outwards from this region...

...Yuina Hirose; Yohei Hirai ABSTRACT Mammary epithelia undergo dramatic morphogenesis after puberty. During pregnancy, luminal epithelial cells in ductal trees are arranged to form well-polarized cystic structures surrounded by a myoepithelial cell layer, an active supplier of the basement membrane...

...Claire Leclech; Carlo F. Natale; Abdul I. Barakat ABSTRACT The basement membrane (BM) is a thin specialized extracellular matrix that functions as a cellular anchorage site, a physical barrier and a signaling hub. While the literature on the biochemical composition and biological activity of the BM...

...Angela M. Howard; Kimberly S. LaFever; Aidan M. Fenix; Cherie' R. Scurrah; Ken S. Lau; Dylan T. Burnette; Gautam Bhave; Nicholas Ferrell; Andrea Page-McCaw ABSTRACT Basement membranes are an ancient form of animal extracellular matrix. As important structural and functional components of tissues...

... signals that pattern the surrounding tissues. It is composed of a core of vacuolated cells surrounded by an epithelial-like sheath of cells that secrete a thick peri-notochordal basement membrane made of different extracellular matrix (ECM) proteins. The correct deposition and organization of the ECM...

... known as MMP14) cell surface exposure at podosome sites. We hypothesise that by promoting podosome formation, VEGF-A enables endothelial cells to overcome the basement membrane barrier to allow sprouting outwards from the existing vasculature. * Present address: Department of Biomedecine...

... inside-out signals to polarise basement membrane assembly at the basal surface, and outside-in signals to control microtubule apical–basal orientation and vesicular trafficking are required for establishing and maintaining the orientation of epithelial cell polarity. Finally, we discuss the relevance...

...Karen K. McKee; Dong-Hua Yang; Rajesh Patel; Zu-Lin Chen; Sidney Strickland; Junichi Takagi; Kiyotoshi Sekiguchi; Peter D. Yurchenco Summary Laminins promote early stages of peripheral nerve myelination by assembling basement membranes (BMs) on Schwann cell surfaces, leading to activation of β1...

... and differentiate to form a stratified epithelium. Using a comparative proteomic approach, we studied this ‘confluence switch’ and identified a new pathway triggered by cell confluence that regulates basement membrane (BM) protein composition by suppressing the uPA–uPAR–plasmin pathway. Indeed, confluence triggers...

... are heterotrimeric glycoproteins of the extracellular matrix that are secreted by epithelial cells and which are crucial for the normal structure and function of the basement membrane. We have generated a mouse harboring a conditional knockout of α3 laminin ( Lama3 fl/fl ), one of the main laminin subunits...

.... The colon also contains crypts of Lieberkühn, but its epithelial surface is lined by flat surface cuffs. The epithelial cells of both organs communicate with the underlying mesenchyme through a basement membrane that is composed of a variety of extracellular matrix proteins, including members...

... microenvironment. The basement membrane molecule, laminin-5 (LN-5), is predominantly found in the medulla of the human thymic lobules. Using high-resolution light microscopy, we show here that LN-5 is localized in a bi-membranous conduit-like structure, together with other typical basement membrane components...

... the LH activity of LH3 developed normally, but showed defects in the structure of the basement membrane and in collagen fibril organization in newborn skin and lung. Analysis of a hypomorphic LH3 mouse line with the same mutation, however, demonstrated that the reduction of the GGT activity of LH3...

..., but not of laminin and nidogen-1, in the basement membrane (BM). Electron immunomicroscopy revealed accumulation of type IV collagen in dilated ERs, but not in the BM of Hsp47 –/– embryos, whereas it was only present in the BM in Hsp47 +/+ embryos. The BM structures stained with anti-laminin and anti-nidogen-1...

...Dirk Breitkreutz; Nicolae Mirancea; Cathrine Schmidt; Regina Beck; Ulrich Werner; Hans-Jürgen Stark; Martin Gerl; Norbert E. Fusenig Basement membranes generally determine different tissue compartments in complex organs, such as skin, playing not only an important structural but also a regulatory...

...Peter D. Yurchenco; Yi-Shan Cheng; Kevin Campbell; Shaohua Li Basement membrane laminins bearing the α2-subunit interact with α-dystroglycan and β1-integrins, cell-surface receptors that are found within the rectilinear costameric lattices of skeletal muscle sarcolemma. Mutations of the α2 subunit...

...Gautam Bhattacharya; Raghu Kalluri; Dana J. Orten; William J. Kimberling; Dominic Cosgrove Usherin is a basement membrane protein encoded by the gene associated with Usher syndrome type IIa, the most common deaf/blind disorder. This report demonstrates a specific interaction between type IV...

... cells were placed on tissue culture plastic; however, the presence of a reconstituted basement membrane in combination with lactogenic hormones partially prevented this decrease. Estrogen receptor αexpression in primary mammary fibroblasts was not altered by these culture conditions, indicating that its...