.... The single mutants tan1 and air9 of Arabidopsis thaliana have minor or no noticeable phenotypes, but the tan1 air9 double mutant has synthetic phenotypes including stunted growth, misoriented divisions and aberrant cell-file rotation in the root differentiation zone. These data suggest that TAN1 plays a role...

... of the TGN/EE and is maintained by the vacuolar H + -ATPase (V-ATPase) with support of proton-coupled antiporters. We show here that ClCd and ClCf, two distantly related members of the Arabidopsis Cl − channel (ClC) family, colocalize in the TGN/EE, where they act redundantly, and are essential for male...

..., composed of HSP70, HSP90, J proteins and other factors. In this study we characterized DJC31 (also known as TPR16) and DJC62 (also known as TPR15) of Arabidopsis thaliana , two J proteins that additionally carry clamp-type tetratricopeptide repeat domains. Using cell fractionation and split GFP, we could...

...–Poc4) in yeast. Pba1–Pba2 initially promotes orderly recruitment of the α-subunits through interactions between their C-terminal HbYX or HbF motifs and pockets at the α 5 –α 6 and α 6 –α 7 interfaces. Here, we identified PBAC5 as a fifth α-ring assembly chaperone in Arabidopsis that directly binds...

... imaging protocols that allow the detailed characterization of CME at multiple scales in plant tissues. These protocols include: (1) an efficient electron microscopy protocol for the imaging of Arabidopsis CME vesicles in situ , thus providing a method for the detailed characterization...

...Laura R. Lee; Dominique C. Bergmann ABSTRACT Stomata are structures on the surfaces of most land plants that are required for gas exchange between plants and their environment. In Arabidopsis thaliana , stomata comprise two kidney bean-shaped epidermal guard cells that flank a central pore...

... high temperature. However, how high temperatures induce the formation of these stress granules in plant cells is largely unknown. Here, we characterized the process of stress granule formation in Arabidopsis thaliana by combining live imaging and electron microscopy analysis. In seedlings grown at 22°C...

... little is known about how they are synthesized and modified. In this Cell Science at a Glance article and accompanying poster, we aim to illustrate the underpinning cell biology of the synthesis of wall carbohydrates, and their incorporation into the wall, in the model plant Arabidopsis...

... of Elongator in early Arabidopsis development in darkness or light (during etiolation or de-etiolation, respectively) by scoring hypocotyl elongation and apical hook formation, two characteristics of seedling growth that differ between the skoto- and photomorphogenetic developmental programs. Seeds of elo3...

.... A quadruple loss-of-function mutant affecting the p24 genes from the δ-1 subclass of the p24δ subfamily ( p24δ3δ4δ5δ6 ) showed alterations in the Golgi, suggesting that these p24 proteins play a role in the organization of the compartments of the early secretory pathway in Arabidopsis . Loss of p24δ-1...

... for pairing sister chromatids during cell division, DNA repair and regulation of gene transcription and silencing. In non-plant species, cohesin is loaded on chromatin by the Scc2–Scc4 complex (also known as the NIBPL–MAU2 complex). Here, we identify the Arabidopsis homolog of Scc4, which we denote...

... orientations within Arabidopsis . Ethylene Cortical microtubule Orientation Hypocotyl growth Arabidopsis Ethylene is a gaseous hormone that plays crucial roles in plant growth, development and stress responses. One of the most widely documented ethylene responses is the triple response...

...Lai-Sheng Meng; Yi-Bo Wang; Shun-Qiao Yao; Aizhong Liu ABSTRACT The Arabidopsis AINTEGUMENTA ( ANT ) gene, which encodes an APETALA2 (AP2)-like transcription factor, controls plant organ cell number and organ size throughout shoot development. ANT is thus a key factor in the development of plant...

... ), to which the phragmoplast appears to be attracted. Defining a role for F-actin has also remained problematic because cell division appears to occur normally in several Arabidopsis mutants affected in the actin cytoskeleton ( Nishimura et al., 2003 ). However, recent research has shown that myosin VIII...

...Ya-Shiuan Lai; Giovanni Stefano; Federica Brandizzi ABSTRACT Whether structure and function are correlated features of organelles is a fundamental question in cell biology. Here, we have assessed the ability of Arabidopsis mutants with a defective endoplasmic reticulum (ER) structure to invoke...

... filaments and addition of preformed fragments at their extremities. Importantly, these data are highly consistent with the modes of bundle formation and growth observed in transgenic Arabidopsis plants expressing a GFP-fused Arabidopsis AtWLIM1 protein. Using two complementary live cell imaging approaches...

... organisms and discuss the role of these organelles in cell polarity and signaling pathways. * Author for correspondence ( bassham@iastate.edu ) © 2012. Published by The Company of Biologists Ltd 2012 Endosome Arabidopsis Endocytosis The eukaryotic endomembrane system functions...

...Carolyn G. Rasmussen; Brian Sun; Laurie G. Smith TANGLED (TAN) is the founding member of a family of plant-specific proteins required for correct orientation of the division plane. Arabidopsis thaliana TAN is localized before prophase until the end of cytokinesis at the cortical division site (CDS...

..., the underlying mechanism by which loss of DJ-1 function contributes to cell death is unclear. Human DJ-1 and its Arabidopsis thaliana homologue, AtDJ-1a, are evolutionarily conserved proteins, indicating a universal function. To gain further knowledge of the molecular features associated with DJ-1 dysfunction...

... in mammals, suggesting the employment of similar evolutionary mechanisms for the regulation of imprinted genes in mammals and flowering plants. * Author for correspondence (e-mail: koehlerc@ethz.ch ) 8 1 2008 © The Company of Biologists Limited 2008 2008 Arabidopsis Epigenetics...