... (also known as SLC7A11). We found that cystine uptake and glutamate export through xCT contributed to rapid NADPH depletion under glucose deprivation. This collapse of the redox system oxidized and inactivated AMP-activated protein kinase (AMPK), a major regulator of metabolic adaptation, resulting...

... pathways, such as sonic hedgehog (SHH) signaling, during embryonic development. Our recent studies have shown that loss of GID ubiquitin ligase function results in aberrant AMP-activated protein kinase (AMPK) activation and elongated primary cilia, which suggests a functional connection to cilia. Here, we...

...Jianhui Li; Mark Hochstrasser ABSTRACT The proteasome is central to proteolysis by the ubiquitin-proteasome system under normal growth conditions but is itself degraded through macroautophagy under nutrient stress. A recently described AMP-activated protein kinase (AMPK)-regulated endosomal sorting...

... to CCCP, rapidly induced the dissolution of Smaug1 MLOs. Metformin and rapamycin elicited similar effects, which were blocked by pharmacological inhibition of AMP-activated protein kinase (AMPK). Finally, we found that Smaug1 MLO dissolution weakens the interaction with target mRNAs, thus enabling...

...-derived cell lines. DRAIC activates AMP-activated protein kinase (AMPK) by downregulating the NF-κB target gene GLUT1, and thus represses mTOR, leading to downstream effects, such as a decrease in protein translation and increase in autophagy. DRAIC, therefore, has an effect on multiple signal...

... branching and fibrosis. Finally, we established the metabolic sensor AMP-activated protein kinase (AMPK) as a critical NaHS target in muscle macrophages. These results identify an interplay between myofibers and macrophages where dystrophic myofibers contribute to the maintenance of a highly inflammatory...

... proteomic approach for the discovery of cellular AMPK targets: Identification of mitochondrial fission factor as a new AMPK substrate . Cell. Signal. 27 , 978 - 988 . 10.1016/j.cellsig.2015.02.008 Frumholtz , L. , Laurent-Roussel , S. , Lipsker , D. and Terrier , B. ( 2020...

..., Drosophila models and genetic tools were used to study the synphilin-1-linked pathways in energy balance by combining molecular biology and pharmacological approaches. We found that expression of human synphilin-1 in flies increased AMP-activated kinase (AMPK) phosphorylation at Thr172 compared...

... followed by mass spectrometry revealed that α subunits of AMPK (α 1 and α 2 , encoded by PRKAA1 and PRKAA2 , respectively) interact with PTP-PEST under normoxia but not in hypoxia. Co-immunoprecipitation experiments confirmed this observation and determined that AMPK α subunits interact with the catalytic...

... kinases suppress NRF2-dependent transcription and NRF2 protein levels in an activity-dependent manner. Integrated phosphoproteomics and RNAseq studies revealed that BRSK2 drives 5′-AMP-activated protein kinase α2 (AMPK) signaling and suppresses the mTOR pathway. As a result, BRSK2 kinase activation...

...Seth Stauffer; Yongji Zeng; Montserrat Santos; Jiuli Zhou; Yuanhong Chen; Jixin Dong ABSTRACT AMP-activated protein kinase (AMPK), a heterotrimeric serine/threonine kinase and cellular metabolic sensor, has been found to regulate cell cycle checkpoints in cancer cells in response to energetic...

...Gabriella M. Forte; Elizabeth Davie; Shervi Lie; Mirita Franz-Wachtel; Ashley J. Ovens; Tingting Wang; Jonathan S. Oakhill; Boris Maček; Iain M. Hagan; Janni Petersen ABSTRACT AMP-activated kinase (AMPK) and target of rapamycin (TOR) signalling coordinate cell growth, proliferation, metabolism...

...Yuan Chi; Kun Gao; Kai Li; Shotaro Nakajima; Satoru Kira; Masayuki Takeda; Jian Yao ABSTRACT Connexin hemichannels regulate many cell functions. However, the molecular mechanisms involved remain elusive. Hemichannel opening causes loss of ATP, we therefore speculated a potential role for AMPK...

..., the molecular mechanism underlying the mitotic fragmentation of the Golgi is far from clear. Here, we show that AMP-activated protein kinase (AMPK) is phosphorylated and activated when cells enter mitosis. Activated AMPK phosphorylates GBF1, a guanine nucleotide exchange factor (GEF) for Arf-GTPases...

.... Published by The Company of Biologists Ltd 2012 Metabolism Stem cells ESCs Reprogramming iPSCs HSCs NSCs AMPK FOXO mTOR SIRT1 Insulin Hypoxia Aging Stem cells serve as the origin for all tissues during embryonic and postnatal development, and contribute to tissue homeostasis...

...Noelia Valbuena; Sergio Moreno The AMP-activated protein kinase (AMPK) is a central regulator of cellular energy homeostasis, which, in response to a fall in intracellular ATP levels, activates energy-producing pathways and inhibits energy-consuming processes. Here, we report that fission yeast...

..., Ser91. Our data indicate that a SPAK-OSR1-independent kinase, perhaps AMP-activated protein kinase (AMPK), phosphorylates Ser130 and that phosphorylation of Thr105 and Ser130 plays the most important roles in stimulating NKCC2 activity. In contrast with NCC, whose membrane translocation is triggered...

...Dong Fu; Yoshiyuki Wakabayashi; Yasuo Ido; Jennifer Lippincott-Schwartz; Irwin M. Arias AMP-activated protein kinase (AMPK), a cellular metabolic sensor, is essential in energy regulation and metabolism. Hepatocyte polarization during liver development and regeneration parallels increased...

...Ivette Aymerich; Fabienne Foufelle; Pascal Ferré; F. Javier Casado; Marçal Pastor-Anglada Adenosine monophosphate (AMP)-activated protein kinase (AMPK) is a heterotrimeric complex that senses intracellular energy status and exerts rapid regulation on energy-demanding and -consuming metabolic...

...Abdallah K. Al-Hakim; Olga Göransson; Maria Deak; Rachel Toth; David G. Campbell; Nick A. Morrice; Alan R. Prescott; Dario R. Alessi The LKB1 tumour suppressor kinase phosphorylates and activates a number of protein kinases belonging to the AMP-activated protein kinase (AMPK) subfamily. We have...