Issues

Summary: The cilium–centrosome complex regulates cell polarity and cell division, but does it help cells decide their fate? We discuss how this complex can influence cells to proliferate, enter quiescence or differentiate.

Summary: Lipid rafts are involved in formation of transport, endocytic, exocytic, synaptic and extracellular vesicles, as well as enveloped viruses. Here, we discuss the proposed mechanisms for these processes.

Summary: The structure of the ER lumen spacer Climp63 was predicted revealing the molecular basis for Climp63 self-association and ER sheet width regulation.

Highlighted Article: Macrophage immune cells phagocytose particulate matter, including pathogens, for killing, digestion and antigen presentation. We present new insights into regulation of this process by Ca²⁺.

Summary: The Sec12 homolog Sed4 interacts with Sec16 and acts to localize Sec16 to ER exit sites. The ER luminal domain of Sed4 is involved in the self-interaction and localization to the highly curved ER domains.

Summary: Manipulation of the Rab7 adaptor protein RILP in macrophages alters extracellular vesicle-mediated cell–cell crosstalk and protects hepatocytes against an injury signal.

Highlighted Article: Fibronectin-bound αvβ3 integrin triggers the dissociation of the RhoA GEF Lfc from microtubules, leading to the activation of RhoA, formation of stress fibres and maturation of focal adhesions.

Summary: Conventional and improved dCas9-mediated CRISPRi methods were used for functional characterization of essential genes in Schizosaccharomyces pombe, showing that this approach is feasible.

Summary: Generation and characterization of human β- and γ-actin from budding yeast as an accessible and low-cost way to study the regulation of specific actin isoforms.