Cover: β-actin filaments present in a field of view from a time-lapse total internal reflection fluorescence (TIRF) microscopy assay. The reaction contained 1 μM β-actin monomers, which polymerised to form actin filaments. The composite image depicts temporally colour-coded actin filaments over a period of ten frames (50 s). For reference, a perfectly static image would have all white filaments, whereas the presence of colour is correlated with filament dynamics. See article by B. K. Haarer et al. (jcs260540).
- PDF Icon PDF LinkTable of contents
- PDF Icon PDF LinkIssue info
The cilium–centrosome axis in coupling cell cycle exit and cell fate
Summary: The cilium–centrosome complex regulates cell polarity and cell division, but does it help cells decide their fate? We discuss how this complex can influence cells to proliferate, enter quiescence or differentiate.
The role of lipid rafts in vesicle formation
Summary: Lipid rafts are involved in formation of transport, endocytic, exocytic, synaptic and extracellular vesicles, as well as enveloped viruses. Here, we discuss the proposed mechanisms for these processes.
Molecular basis of Climp63-mediated ER lumen spacing
Summary: The structure of the ER lumen spacer Climp63 was predicted revealing the molecular basis for Climp63 self-association and ER sheet width regulation.
Rab GTPase regulation of phagosome–lysosome fusion is bypassed in the presence of micromolar Ca2+
Highlighted Article: Macrophage immune cells phagocytose particulate matter, including pathogens, for killing, digestion and antigen presentation. We present new insights into regulation of this process by Ca2+.
Sec16 and Sed4 interdependently function as interaction and localization partners at ER exit sites
Summary: The Sec12 homolog Sed4 interacts with Sec16 and acts to localize Sec16 to ER exit sites. The ER luminal domain of Sed4 is involved in the self-interaction and localization to the highly curved ER domains.
Inflammatory macrophage to hepatocyte signals can be prevented by extracellular vesicle reprogramming
Summary: Manipulation of the Rab7 adaptor protein RILP in macrophages alters extracellular vesicle-mediated cell–cell crosstalk and protects hepatocytes against an injury signal.
Lfc subcellular localization and activity is controlled by αv-class integrin
Highlighted Article: Fibronectin-bound αvβ3 integrin triggers the dissociation of the RhoA GEF Lfc from microtubules, leading to the activation of RhoA, formation of stress fibres and maturation of focal adhesions.
TOOLS AND RESOURCES
Genetic knockdown of genes that are obscure, conserved and essential using CRISPR interference methods in the fission yeast S. pombe
Summary: Conventional and improved dCas9-mediated CRISPRi methods were used for functional characterization of essential genes in Schizosaccharomyces pombe, showing that this approach is feasible.
Purification of human β- and γ-actin from budding yeast
Summary: Generation and characterization of human β- and γ-actin from budding yeast as an accessible and low-cost way to study the regulation of specific actin isoforms.
Reasons to submit to Journal of Cell Science
There are many benefits to publishing through Journal of Cell Science, follow this link to read more about our reasons to submit or visit our submission page now.
Call for papers: Cell and Tissue Polarity
We are welcoming submissions for our next special issue, which will focus on ‘Cell and tissue polarity’ and will be guest edited by David Bryant. Submission deadline: 15 July.
The Forest of Biologists
The Forest of Biologists is a biodiversity initiative created by The Company of Biologists, with support from the Woodland Trust. For every Research and Review article published in Journal of Cell Science a native tree is planted in a UK forest. In addition to this we are protecting and restoring ancient woodland and are dedicating these trees to our peer reviewers. Visit our virtual forest to learn more.
Propose a new Workshop for 2025
Do you have an idea for a Workshop? We are now accepting proposals for our 2025 Biologists Workshops programme. As the scientific organiser, your involvement will be focused on the science. We'll take care of all the logistics. In 2025 we'll continue our efforts to diversify our Workshop programme and will be reserving one of our Workshops for an application from a Global South (GS) country to host an event overseas.
FocalPlane Image Competition 2023
FocalPlane’s 2023 image competition is underway, in celebration of the community site’s third birthday. Click here for submission details and information on how to showcase your favourite image.