Issues

Summary: A discussion of the molecular mechanisms of the removal of protein aggregates by the selective autophagy pathway termed aggrephagy. We describe its regulation, focusing on neuronal cells, and potential therapeutic manipulation.

Summary: Autophagy is an essential process that is implicated in several diseases. We discuss recent findings that uncover regulators of autophagy in mammalian cells with a focus on cancer.

Highlighted Article: Single-cell intracellular pH (pHi) measurements reveal oscillations during the mammalian cell cycle. Manipulating pHi alters phase lengths and transitions, suggesting pHi dynamics regulate the cell cycle.

Summary: The BAR domain protein pacsin 2 plays an essential role in collective cell migration of cancer cells by regulating internalization of N-cadherin.

Summary: The histone H3/H4 chaperone CHAF1B prevents mislocalization of CENP-A to non-centromeric regions, thus preventing chromosomal instability.

Summary: Single-molecule analysis of DNA replication in Giardia lamblia.

Summary: Upon glucose exhaustion, yeast cells exit the cell cycle and enter stationary phase through CDK inhibition. However, we show that CDK actively contributes to the stationary phase establishment.

Highlighted Article: GIPC3 couples the molecular motors MYO6 and MYO18A to actin cytoskeleton structures in hair cells. GIPC3 is necessary for shaping cuticular plate of the hair cell and hence the arrangement of the stereocilia in the hair bundle.

Summary: The E2 ubiquitin-conjugating enzymes UBE2D1 and UBE2D2 modulate the levels of VEGFR2, which regulates endothelial function and angiogenesis.

Summary: Nonsense-mediated mRNA decay utilizes a coupled protein quality control pathway to coordinate degradation of both the aberrant mRNA and its associated nascent polypeptide chain.

Summary: The conserved yeast mitochondrial inner membrane protein Cqd1 interacts with the outer membrane proteins Por1 and Om14. Additionally, Cqd1 is important for maintaining mitochondrial homeostasis.

Summary: The ceramide synthase homolog Tlc4 suppresses cell lethality associated with an aberrant nuclear membrane. Translocation of Tlc4 from the endoplasmic reticulum to the Golgi is linked to its suppressive activity.

Highlighted Article: A systematic, cell-based comparison of G-protein-binding domains for their potential as localization-based, genetically encoded biosensors for imaging of Cdc42 or Rac activity in single cells.